Biomedical Science Letters

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Fig. 1.

Isolation and characterization of breast cancer stem cells (BCSCs). (A) Morphology of BCSCs from MCF-7 cells at 7, 14 and 21 days of culture, observed using the microscope. MCF-7 cells were capable of forming BCSCs of about 50 mm in diameter under BCSCs culture condition for 1 week. (B) BCSCs and MCF-7 cells were analyzed using fluorescence activated cell sorting (FACS) and showed cell surface proteins. BCSCs at 14 days showed higher levels of CD44+ CD24-/low populations than MCF-7 cells or BCSCs at 7 and 21 days cells.

Biomed Sci Letters 2019;25:159-69 https://doi.org/10.15616/BSL.2019.25.2.159
© 2019 Biomed Sci Letters