Biomedical Science Letters

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Fig. 4.

Anti-proliferation effect of dual drug-loaded liposomes on BCSCs. The growth of the isolated CD44-/low/CD24+ MCF-7 tumor cells (A) and the isolated CD44+/CD24-/low MCF-7 BCSCs (B) treated with free drugs (Dox, Met, Sod, and Met+Sod) or liposomal encapsulated drugs (Lipo-Dox, Lipo-Met, Lipo-Sod, and Lipo-[Met+Sod]) for 48 hours was determined by WST-1 assay. The data were calculated as percentage of contol (DPBS). The colonies formed in BCSCs-seeded 6-well plate after treatment of various free drugs or liposomal encapsulated drugs for 14 days were stained with crystal violet (0.5% w/v) and obserbved by a bright-field microscope (C) and quantfied (D). Data shown represent the mean ± SD of three experiments (*P<0.05, **P<0.01). Abbreviations: BCSCs, breast cancer stem cells; DPBS, Dulbecco’s phosphate buffered saline; Dox, doxorubicin; Met, metformin; Sod, sodium salicylate, Lipo-, liposomal encapsulated; SD, standard deviation.

Biomed Sci Letters 2019;25:159-69
© 2019 Biomed Sci Letters