Biomedical Science Letters

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Fig. 1.

R239Q mutants induced the intracellular accumulation and aggregation of CCN3. (A) Schematic of mouse CCN3-R239 mutants. (B) Western blot analysis of CCN3-R239 mutations in Neuro2a cells. The open arrowheads indicate the monomer of CCN3 and the solid arrowheads indicate the aggregated form of CCN3. WCL; Whole cell lysate, CM; Conditioned Media. (C) In vivo analysis of callosal projection formation upon expression of CCN3-R239Q mutant. In utero electroporation was performed at E14.5 with plasmids expressing the CCN3-R239Q and GFP. The open arrowheads indicate the normal paths of L1-positive callosal axons. In contrast, the solid arrowheads indicate the path of CCN3-R239Q-overexpressing neurons. Scale bar, 2 mm. Ctx; Cortex, CC; Corpus callosum.

Biomed Sci Letters 2018;24:64-75 https://doi.org/10.15616/BSL.2018.24.2.64
© 2018 Biomed Sci Letters