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Inhibitory Effects of Water Extract from Rice Bran Due to cAMP-dependent Phosphorylation of VASP (Ser157) on ADP-induced Platelet Aggregation
2014;20:129-138
Published online September 30, 2014;  https://doi.org/10.15616/BSL.2014.20.3.129
© 2014 The Korean Society For Biomedical Laboratory Sciences.

Hyun-Hong Kim1, Jeong Hwa Hong2, Pajaree Ingkasupart2, Dong-Ha Lee1,3 and Hwa-Jin Park1,

1Department of Biomedical Laboratory Science, Inje University, Gyungnam 621-749, Korea
2Department of Smart Foods and Drugs, Inje University, Gyungnam 621-749, Korea
3Department of Biomedical Laboratory Science, Korea Nazarene University, Chungnam 331-718, Korea
Correspondence to: Hwa-Jin Park
Department of Biomedical Laboratory Science, College of Biomedical Science and Engineering, Inje University, 197, Inje-ro, Gimhae, Gyungnam 621-749, Korea.
Tel: +82-55-320-3538, Fax: +82-55-334-3426
e-mail: mlsjpark@inje.ac.kr
Received June 2, 2014; Revised September 16, 2014; Accepted September 27, 2014.
 Abstract
In this study, we investigated the effect of water extract from rice bran (RB) on ADP (20 關M)-stimulated platelet aggregation. RB dose-dependently inhibited ADP-induced platelet aggregation, and its IC50 value was 224.0 關g/mL, which was increased by adenylate cyclase inhibitor SQ22536 and cAMP-dependent protein kinase (A-kinase) inhibitor Rp-8-Br-cAMPS. RB elevated the phosphorylation of VASP (Ser157) which was also inhibited by SQ22536 and Rp-8-Br-cAMPS. It is thought that RB-elevated cAMP contributed to the phosphorylation of VASP (Ser157) to inhibit ADP-induced platelet aggregation. Therefore, we demonstrate that RB has an antiplatelet effect via cAMP-dependent phosphorylation of VASP (Ser157), and RB may have preventive or therapeutic potential for platelet aggregation-mediated diseases, such as thrombosis, myocardial infarction, atherosclerosis, and ischemic cerebrovascular disease.
Keywords : Rice bran, Platelet aggregation, cAMP-dependent protein kinase, cAMP, Vasodilator-stimulated phosphoprotein- Ser157 phosphorylation