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Expression of Lysozyme and Aquaporins mRNA in Otitis Media
Biomed Sci Letters 2018;24:116-124
Published online June 30, 2018;
© 2018 The Korean Society For Biomedical Laboratory Sciences.

Sung-Ho Kang1, Dae-Joon Lim1, Bo-Hyung Kim1, Myung-Sang Rhu1 and Shin-Seok Kang2,†

1Department of Otolaryngology, Chunju Hospital, Kunkuk University School of Medicine, Chungju 27376, Korea
2Chungbuk Veterinary Service Laboratory, Chungju 27336, Korea
Correspondence to: Shin-Seok Kang. Chungbuk Veterinary Service Laboratory, 13 Paenggorisan-gil, Chungju-si, Chungchengbuk-do 27336, Korea.
Tel: +82-43-220-6311, Fax: +82-43-220-6319, e-mail:
Received May 3, 2018; Revised June 20, 2018; Accepted June 21, 2018.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Lysozyme is known as a key substance of the innate immunity and have antibacterial effect in the mucosal tissues, especially middle ear. Aquaporin (AQP) functions as water movement in the tissue and has been expected to be participated in the inflammatory responses. In the present study, we investigated to reveal association of lysozymes and AQPs in otitis media. The gene expression of lysozyme genes, homo sapiens lysozyme (hLYZ), homo sapiens lysozyme M (hLYZ M), and homo sapiens lysozyme G like-2 (hLYGH), and AQP genes (AQP 0 - AQP 12) were measured from postauricular skin, mastoid mucosa, inflamed mastoid mucosa, and middle ear mucosa. The hLYZ, hLYZ M and hLYGH gene were expressed in mastoid mucosa, inflamed mastoid mucosa, middle ear mucosa. Of AQP genes, all AQP gene except AQP 3 gene were expressed in the tissue of middle ear. Among them, AQP 4, AQP 8, AQP 9, AQP 10, AQP 11 and AQP 12 were highly expressed in the inflamed mastoid mucosa and normal mastoid mucosa (P<0.001). Interestingly, expression levels of AQP 4, AQP 9, and AQP 12 gene were significantly higher in the inflamed mastoid mucosa compared to normal middle ear mucosa (P<0.05). These results suggest that lysozyme and AQPs could be associated with inflammatory response in the middle ear.
Keywords : Otitis media, Lysozyme, Aquaporin (AQP), Inflamed mastoid mucosa