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Assessment of the Cardioprotection Offered by Fisetin in H2O2-induced Zebrafish (Danio rerio)-Tg (cmlc2: egfp)
Biomed Sci Letters 2018;24:130-133
Published online June 30, 2018;
© 2018 The Korean Society For Biomedical Laboratory Sciences.

Jeong-Soo Lee1, Eun-Seok Park2,† and In-Sik Kim1,3,†

1Department of Senior Healthcare, BK21 Plus Program, Graduate School, Eulji University, Daejeon 34824, Korea
2Department of Biomedical Laboratory Science, Kyungbok University, Gyeonggi-do 12051, Korea
3Department of Biomedical Laboratory Science, School of Medicine, Eulji University, Daejeon 34824, Korea
Correspondence to: In-Sik Kim. Department of Biomedical Laboratory Science School of Medicine, Eulji University 77, Gyeryoung-ro 771 beon-gil, Jung-Gu, Daejeon 34824, Korea.
Tel: +82-42-259-1753, Fax: +82-42-259-1759, e-mail:
Eun-Seok Park. Department of Biomedical Laboratory Science, Kyungbok University, 425, Kyeongbokdae-ro, Jinjeop-eup, Namyangju-si, Gyeonggi-do 12051, Korea.
Tel: +82-31-539-5430, Fax: +82-31-570-9929, e-mail:
Received December 21, 2017; Revised May 29, 2018; Accepted June 15, 2018.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
The aim of this study was to evaluate the protective function of fisetin, a natural flavonoid in zebrafish heart for the treatment of myocardial infarction in coronary and ischemic heart disease. For this purpose, we induced oxidative stress zebrafish (Danio rerio)-Tg (cmlc2: egfp) by H2O2 and then administered fisetin, the protective effect of fisetin was determined by measuring the heart rate following fisetin administration. After testing the toxicity of fisetin, we found that the heartt increased in a concentration-dependent manner, however there was no difference between the heart rates of embryos and adults. The improved heart rate demonstrated the cardioprotective effect of fisetin. The result showed that fisetin, at concentration of 3and 5 μM, significantly increased heart rate compared with the heart with H2O2 alone. This indicates that fisetin plays an important role in the prevention of heart damage and treatment of cardiovascular diseases caused by oxidative stress due to ischemia / reperfusion.
Keywords : Fisetin, Zebrafish (Danio rerio)-Tg (cmlc2: egfp), Cardiovascular disease, H2O2