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Noninvasive Detection of Specific Diagnostic Biomarkers for Atopic Dermatitis
Biomed Sci Letters 2019;25:15-22
Published online March 31, 2019;  https://doi.org/10.15616/BSL.2019.25.1.15
© 2019 The Korean Society For Biomedical Laboratory Sciences.

Jeong Hyun Chang†,*

Department of Clinical Laboratory Science, Daegu Haany University, Gyeongsan-si 38610, Korea
Correspondence to: Jeong Hyun Chang. Department of Clinical Laboratory Science, Daegu Haany University, Gyeongsan-si 38610, Korea.
Tel: +82-53-819-1350, Fax: +82-53-819-1353, e-mail: jhchang@dhu.ac.kr
Received January 28, 2019; Revised March 3, 2019; Accepted March 14, 2019.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
 Abstract
The diagnosis of atopic dermatitis (AD) includes a test that checks allergen-mediated skin reactions and a method of measuring the total IgE and allergen-specific IgE in blood. However, these test methods are performed directly on the patient, which cause some pain or discomfort. In addition, the skin response test or IgE may result in false negative in about 20% of patients. In the present study, to identify specific biomarkers, HaCaT cells were used as a human keratinocyte that make up the skin, were treated IL-4 and IL-13 for 24 hours to induce a situation similar to keratinocytes in AD patients. In the HaCaT cells, pro-inflammatory cytokine such as IL-5, IL-6, and MCP-1 were increased by IL-4 and IL-13 and skin barrier proteins was reduced by IL-4 and L-13. This results showed that a situation similar to the stratum corneum of an actual patient is induced in HaCaT cells. And then the secretions of Kallikrein (KLK) 5 and KLK7 protease were checked by enzyme-linked immunosorbent assay (ELISA). It was specifically increased by IL-4 and IL-13. This showed that AD-related protease can be detected at the protein level using keratinocytes that can be taken in a non-invasive manner and suggested the possibility of applying it to AD diagnosis.
Keywords : Atopic dermatitis, Keratinocyte, Diagnosis, Kallikrein 5, Kallikrein 7