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Mycological Features of Trichophyton verrucosum Isolated in Cattle
Biomed Sci Letters 2019;25:367-371
Published online December 31, 2019;  https://doi.org/10.15616/BSL.2019.25.4.367
© 2019 The Korean Society For Biomedical Laboratory Sciences.

Su Jung Kim,*

Department of Biomedical Laboratory Science, Daegu Health College, Daegu 41453, Korea
Correspondence to: Su Jung Kim. Department of Biomedical Laboratory Science, Daegu Health College, Youngsong-ro, Daegu 41453, Korea.
Tel: +82-53-320-1303, Fax: +82-53-320-1450, e-mail: sjkim4238@hanmail.net
*Professor.
Received November 20, 2019; Revised December 5, 2019; Accepted December 9, 2019.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
 Abstract

In this study, the author observed the fungal characteristics of T. verrucosum which is commonly known as the cattle ringworm fungus in the farms of Hoengseong, Gangwon-do. After isolating 20 strains of T. verrucosum from cattle, they were cultured on SDA, PDA media and the fungal characteristics were concluded through visual observation of the colonies, microscopic findings, hair perforation test and urease test. The size of the colonies cultured on SDA media at 37 C was on an average 5 mm at 1 week, 33 mm at 2 weeks, 42 mm at 3 weeks and 58 mm after 4 weeks. Observing the characteristics of the colonies, 17 strains of T. album showing central bold radial folds, 2 strains of T. ochraceum having l throughout the colonies and 1 strain of T. discoides with rapid growth rate and gray-white cotton patterns were found. On microscopic observations of the cultured colonies on SDA, PDA media, macroconidia and microconidia were not found in T. verrucosum and hyphae and chlamydospore were only seen in T. album types. Out of 20 strains of T. verrucosum, hair perforation test was positive on only 3 strains and urease test was positive on all of the 20 strains.

Keywords : Dermatophytoses, Zoophilic fungus, Trichophyton verrucosumi
꽌 濡

Trichophyton verrucosum 냼뿉 踰꾩쭚쓣 씪쑝궎뒗 뵾遺 湲곗깮꽦 怨고뙜씠硫 媛곸쭏쓣 遺꾪빐븯뿬 媛뵾瑜 삎꽦븯怨 씤泥 媛먯뿼 떆 몢遺諛깆꽑쓣 쑀諛쒗븯뒗 룞臾쇱튇솕꽦 吏꾧퇏씠떎(Lee et al., 2018). 꽭怨꾩쟻쑝濡 諛쒖깮븯뒗 T. verrucosum 뿰졊, 꽦蹂꾩뿉 愿怨꾩뾾씠 諛쒖깮븯굹 듅엳 깮썑 2뀈 씠븯쓽 냼뿉꽌 遺꾨━쑉씠 넂쑝硫 냼瑜 궗쑁, 젒珥, 洹몃━怨 룄異뺤뾽옄뱾뿉寃 諛쒖깮鍮덈룄媛 넂寃 蹂닿퀬릺뿀떎(Shin et al., 1998; Zhan and Liu, 2017). 슦由щ굹씪뒗 1977뀈 寃쎌긽遺곷룄 湲덈=援곗쓽 븳 븳슦 紐⑹옣뿉꽌 吏묐떒쟻쑝濡 諛쒖깮븳 냼 諛깆꽑쑝濡쒕꽣 泥섏쓬 T. verrucosum쓣 遺꾨━븯怨 궗엺뿉꽌쓽 媛먯뿼 1986뀈 쟾궓 吏뿭뿉꽌 븳슦濡쒕꽣 쟾뿼맂 몢遺 룆李쎌쓽 泥 삁瑜 蹂닿퀬븯떎(Choi et al., 1979; Kim et al., 1986). 궗엺쓽 T. verrucosum 媛먯뿼 遺遺 냼 諛깆꽑쑝濡쒕꽣 吏곸젒 媛먯뿼릺뒗 寃껋쑝濡 옒 븣젮吏怨 엳怨 쓽蹂 벑뿉 삤뿼릺뼱 媛議깆뿉寃 쟾뿼떆궎뒗 以묒슂븳 留ㅺ컻泥닿 맆 寃껋쑝濡 異붿륫릺硫 二쇰줈 뼹援, 뙏, 紐, 몢遺 벑쓽 닚꽌濡 諛쒖깮븳 寃껋쑝濡 蹂닿퀬뻽떎(Choi and Lee, 2006; Kim et al., 2006; Lee et al., 2018).

援궡뿉꽌뒗 1992뀈 源 벑뿉 쓽빐 냼뿉꽌 遺꾨━맂 T. verrucosum쓽 뿭븰쟻, 吏꾧퇏븰쟻 냼寃ъ쓣 諛쒗몴븳 씠옒濡 T. verrucosum뿉 븳 諛쒗몴媛 쟾臾댄븯뿬 蹂 뿰援ъ쭊 냼뿉꽌 遺꾨━맂 T. verrucosum쓽 삎깭븰쟻 꽦긽쓣 議곗궗븯뿬 뼢썑 룞臾쇱튇솕꽦씤 T. verrucosum쓽 遺꾨━뿉 엳뼱 湲곗큹옄猷뚯뿉 룄씠 릺怨좎옄 떎떆븯떎(Kim et al., 1992).

옱猷 諛 諛⑸쾿

옱猷

2018뀈 01썡遺꽣 12썡 31씪源뚯 媛뺤썝룄 슒꽦뿉꽌 궗쑁븯뒗 냼 踰꾩쭚 利앹긽쓣 굹궡뒗 냼瑜 긽쑝濡 냼룆맂 븬꽕옄瑜 씠슜븯뿬 媛곸쭏, 媛뵾, 洹몃━怨 뵾遺瑜 닔吏묓븯쑝硫 씠윴 寃泥대 Sabouraud dextrose agar (SDA), Potato dextrose agar (PDA)] urea dextrose agar瑜 씠슜븯뿬 T. verrucosum 20洹좎<瑜 遺꾨━븯떎(Kim et al., 1992; Lee et al., 2018).

諛⑸쾿

쑁븞쟻 냼寃: SDA 룊뙋諛곗뿉 젒醫 썑 37℃뿉꽌 諛곗뼇븯怨 吏묐씫쓽 媛옣 겙 吏곴꼍쓣 留ㅼ<留덈떎 mm 떒쐞濡 痢≪젙븯뿬 4二쇨퉴吏쓽 꽦옣 젙룄瑜 愿李고븯怨 諛곗뼇 4二쇱㎏뿉 吏묐씫쓽 쟾硫댁쓣 愿李고븯뿬 삎깭뿉 뵲씪 遺꾨쪟븯떎(Lee et al., 2018).

쁽誘멸꼍 寃궗: SDA 諛 PDA 룊뙋諛곗뿉 젒醫낇븯怨 37℃뿉꽌 諛곗뼇븯뿬 젒醫 1二쇰꽣 4二쇨퉴吏 scotch tape method濡 吏묐씫쓽 몴硫댁쓣 梨꾩랬, lactophenol cotton blue濡 뿼깋븯뿬 쁽誘멸꼍븯뿉꽌 愿李고븯떎. 삉븳 37℃뿉꽌 SDA PDA瑜 궗슜븯뿬 slide culture瑜 떎떆븯떎(Bang and Kim, 2007).

紐⑤컻泥쒓났 寃궗: 紐⑤컻 硫멸퇏븳 썑 洹좎<媛 젒醫낅맂 SDA 룊뙋諛곗뿉 肉뚮젮꽌 諛곗뼇 1二쇱 2二 썑뿉 紐⑤컻쓽 泥쒓났 뿬遺瑜 쁽誘멸꼍쑝濡 寃궗븯떎(Hong et al., 2018).

Urea 떆뿕: Uera dextrose agar뿉 젒醫 썑 2二 룞븞 urea 遺꾪빐 뿬遺瑜 愿李고븯떎(Kim et al., 1992).

寃 怨

쑁븞쟻 諛 쁽誘멸꼍쟻 냼寃

37℃뿉꽌 SDA 룊뙋諛곗뿉 諛곗뼇븯뿬 愿李고븳 寃곌낵 꽦옣냽룄뒗 룊洹 洹좎쭛씫쓽 겕湲곌 1二 3~8 mm(룊洹 5 mm), 2二쇱뿉 20~45 mm(룊洹 33 mm), 3二쇱뿉 30~55 mm(룊洹 42 mm), 洹몃━怨 4二 썑뿉뒗 50~63 mm(룊洹 58 mm)濡 굹궗떎(Table 1).

Growth rate on Sabouraud dextrose agar at 37℃

Week after inoculationSize of colonies (mm)

RangeMean
13~8 5
220~4533
330~5542
450~6358

삎깭븰쟻 듅吏뺤쓣 愿李고븯湲 쐞븯뿬 SDA 룊뙋諛곗뿉 냼뿉꽌 遺꾨━븳 T. verrucosum 20洹좎<瑜 젒醫낇븯뿬 4二쇨컙 諛곗뼇븳 썑 吏묐씫쓣 愿李고븳 寃곌낵 겕寃 3媛吏濡 굹궗떎.

1삎 吏묐씫쓽 以묒븰遺뿉꽌 떆옉릺뒗 援듭 諛⑹궗긽쓽 二쇰쫫씠 굹굹硫 二쇱쐞濡 六쀬뼱 굹媛뒗 삎쑝濡 17洹좎<(T. verrucosum varalbum)媛 遺꾨━릺뿀怨 쁽誘멸꼍 냼寃ъ 遺꾩깮옄 냼遺꾩깮옄뒗 蹂 닔媛 뾾뿀怨 썑留됲룷옄뒗 궗뒳紐⑥뼇쓽 쟾삎쟻씤 뿼二쇳삎깭媛 留롮씠 愿李곕릺뿀떎.

2삎 吏묐씫쓽 쟾諛섏뿉 嫄몄퀜 誘몄꽭 二쇰쫫씠 굹굹硫 뿰븳 솴깋쓽 吏묐씫쑝濡 2洹좎<(T. verrucosum var ochraceum)媛 遺꾨━릺뿀怨 쁽誘멸꼍 냼寃ъ 遺꾩깮옄 냼遺꾩깮옄, 洹몃━怨 궗뒳紐⑥뼇쓽 썑留됲룷옄룄 蹂 닔 뾾뿀怨 洹좎궗쓽 留먮떒遺遺꾩뿉 떒씪긽쓽 썑留됲룷옄瑜 蹂 닔 엳뿀떎.

3삎 꽦옣냽룄媛 媛옣 鍮좊Ⅴ怨 鍮꾧탳쟻 씗怨 렪룊븯硫 二쇰쫫씠 뾾뒗 썝뙋삎 吏묐씫씠硫 씛깋쓽 넑꽭紐⑥뼇쓽 洹좎궗媛 엳뒗 삎쑝濡 1洹좎<(T. verrucosum var discoides)媛 遺꾨━릺뿀떎. 쁽誘멸꼍 냼寃ъ 遺꾩깮옄 냼遺꾩깮옄뒗 蹂 닔媛 뾾뿀怨 洹좎궗媛 뭾遺븯硫 궗뒳紐⑥뼇 썑留됲룷옄뒗 蹂 닔 뾾뿀怨 븘二 뱶臾쇨쾶 떒씪긽 썑留됲룷옄瑜 蹂 닔 엳뿀떎(Fig. 1 & Table 2, 3).

Colony morphology on Sabouraud Dextrose Agar at 37℃

T. verrucosum var
album (n=17)ochraceum (n=2)discoides (n=1)
ColorGray - whiteYellowGray - white
SurfaceFlat, glabrous radial - folded button like centerFlat and glabrousFlat and cotton
Colony morphology

Results of slide culture on Sabouraud Dextrose Agar

Microscopic featuresT. verrucosum var

album (n=17)ochraceum (n=2)discoides (n=1)
Microconidia---
Macroconidia---
Chlamydospores+++++

Fig. 1. Microscopic features of Trichophyton verrucosum (Lactophenol cotton blue, 횞400 Microscope).

A: Type I, B: Type II, C: Type III.


紐⑤컻泥쒓났 寃궗 諛 urease 떆뿕

紐⑤컻泥쒓났 寃궗뒗 SDA 諛곗뿉 洹좎쓣 젒醫낇븳 썑 37℃뿉꽌 諛곗뼇븯뿬 洹좎궗媛 옄씪뒗 寃껋쓣 솗씤븳 썑 냼룆맂 紐⑤컻쓣 洹좎궗媛 옄씪뒗 遺쐞뿉 肉뚮젮꽌 2二쇨컙 諛곗뼇 썑 愿李고븳 寃곌낵 珥 20洹좎< 以 17 (85%)洹좎<뿉꽌 紐⑤컻泥쒓났 諛섏쓳 쓬꽦씠뿀怨 3 (15%)洹좎<媛 떎諛쒖꽦쓽 紐⑤컻泥쒓났 諛섏쓳 뼇꽦쓣 솗씤븷 닔 엳뿀떎(Fig. 2).

Fig. 2. Hair perforation test on SDA at 37꼦.

Sites of hair perforation (arrow key, ×400 Microscope).


Urease 떆뿕 uera dextrose agar 궗硫대같吏뿉 젒醫 썑 2二 룞븞 urea 遺꾪빐 뿬遺瑜 愿李 寃곌낵 20二 紐⑤몢媛 쓬꽦쑝濡 굹궗떎. Urease 뼇꽦씤 T. mentagrophytes 쓬꽦씤 T. rubrum쓣 議곌퇏쑝濡 媛숈씠 궗硫대같吏뿉 젒醫낇븯뿬 쓬꽦怨 뼇꽦쓣 媛먮퀎븯떎(Fig. 3 & Table 4).

Results of urease test and hair perforation test

Colony typeUrease testHair perforation


+-+-
T. album (n=17)- 7-17
T. ochraceum (n=2)- 22-
T. discoides (n=1)- 11-

Total020317

Fig. 3. Urease test.

A: T. mentagrophytes (positive control), B: T. rubrum (negative control), C: T. verrucosum (-)


怨 李

룞臾쇱튇솕꽦 吏꾧퇏씤 T. verrucosum 媛먯뿼 룞젅湲곕 以묒떖쑝濡 뒭媛쓣遺꽣 遊꾩뿉 留롮씠 諛쒖깮븯硫 媛먯뿼슂씤쑝濡쒕뒗 냼쓽 뿰졊, 硫댁뿭, 쁺뼇긽깭 궗쑁솚寃쎌씠硫 二쇰줈 異뺤궗궡遺쓽 솚湲, 뒿룄, 洹몃━怨 吏묐떒 궗쑁 벑씠 쐞깮쟻씠吏 紐삵븷 寃쎌슦 굹굹硫 씠濡 씤빐 媛먯뿼맂 냼瑜 궗쑁븯嫄곕굹 젒珥됲븯쓣 븣 씤泥댁쑀諛쒖쓣 씪쑝궓떎(Choe et al., 2006). 냼쓽 媛먯뿼珥덇린뿉뒗 옉 쉶諛깆깋쓽 諛섏젏쑝濡 떆옉븯뿬 깉紐④ 떆옉릺硫 깉紐⑥쓽 吏곴꼍씠 1~10 cm 젙룄濡 而ㅼ怨 깉紐⑤쐞뿉뒗 뫁湲怨 뵳뵳븳 媛뵾媛 삎꽦맂떎(Kim et al., 2002; Hong et al., 2018).

T. verrucosum쓽 遺꾨쪟뒗 Sabouraud媛 maltose 諛곗뿉꽌 솴넗깋쓽 李⑹깋쓣 굹궡뒗 T. ochraceum, T. albumT. discoides뿉 븯뿬 湲곗닠븯怨 1934뀈 Emmons 벑 T. verrucosum쓽 룷옄 遺냽湲곌쓽 삎깭뿉 뵲씪 遺꾨쪟븯쑝硫 T. album, T. ochraceum, 洹몃━怨 T. discoides 媛숈 洹좎<엫쓣 蹂닿퀬븯떎(Emmons, 1934; Lü, 1961). T. verrucosum 삎깭뿉 뵲씪 떎삩 諛곗뼇 떆 吏묐씫쓽 꽦옣씠 留ㅼ슦 뒓由ш퀬 怨듭쨷洹좎궗媛 쟻怨 쑖湲곕맂 吏묐씫쓽 紐⑥뼇씠 씛깋쓽 떒異붾え뼇쓣 븳 寃껋쓣 T. album 삎, 렪룊븳 솴깋쓣 쓣뒗 吏묐씫쓣 T. ochraceum 삎 洹몃━怨 렪룊븯怨 怨듭쨷洹좎궗媛 떎냼 굹굹硫 뿰븳 쉶깋쓽 吏묐씫쓣 굹궡뒗 T. discoides 삎쑝濡 꽭 媛吏濡 遺꾨쪟릺뿀떎(Choi et al., 1979). 理 벑(Choe et al., 2006)쓽 19뀈媛 T. verrucosum 媛먯뿼利앹쓽 썑뼢쟻 愿李곗뿉 쓽븯硫 냼뿉꽌뒗 二쇰줈 븞硫, 늿 二쇱쐞, 몢遺 벑쓽 遺쐞뿉 씗怨 몢爰쇱슫 媛뵾꽦 씤꽕怨 깉紐⑤컲쑝濡 굹굹硫 媛먯뿼맂 냼濡쒕꽣 씤泥 媛먯뿼 떆 蹂묐 쟾泥댁뿉 뿼利앷낵 궪異쒖씠 떖븳 쑖빀꽦 썝삎 뙋 諛 몢遺 룆李쎌쑝濡 굹궃떎(Choe et al., 2006). 源 벑(Kim et al., 1992) T. verrucosum 떎삩뿉꽌 3~4二 諛곗뼇븷 寃쎌슦뿉 吏묐씫쓽 겕湲곌 10~15 mm 젙룄씪怨 븯쑝硫 씠윭븳 꽦옣냽룄媛 뒓由щ濡 쟻뼱룄 4二쇨컙 諛곗뼇븯뿬 吏묐씫쓣 愿李고븯뿬 룞젙씠 媛뒫븯굹 삤뿼쑝濡 遺꾨━ 룞젙씠 돺吏 븡븘꽌 蹂 洹좎뿉 쓽븳 媛먯뿼利앹쓽 떎젣 鍮덈룄뒗 뜑 넂쓣 媛뒫꽦쓣 蹂닿퀬븯떎(Kim et al., 1992).

遺遺꾩쓽 뵾遺궗긽洹좎 25℃뿉꽌 諛곗뼇븯뒗 寃껋쓣 썝移숈쑝濡 븯硫 37℃뿉 諛곗뼇븯硫 吏묐씫쓽 꽦옣씠 뼲젣릺怨 李⑹깋씠 옒 굹굹吏 븡뒗 諛섎㈃ T. verrucosum 25℃蹂대떎 37℃뿉 諛곗뼇븯硫 옒 꽦옣븯뒗 듅吏뺤씠 엳떎(Choi and Lee, 2006; Choe et al., 2006).

蹂 뿰援ъ쭊룄 븵꽑 끉臾몄쓽 떎뿕 꽦쟻쓣 鍮꾧탳븯湲 쐞븯뿬 떎뿕議곌굔쓣 37℃뿉꽌 떎떆븯떎(Choi and Lee, 2006; Choe et al., 2006). SDA 룊뙋諛곗뿉꽌 吏묐씫쓽 겕湲곕 愿李고븳 寃곌낵 1二 룊洹 5 mm, 2二쇱뿉뒗 룊洹 33 mm, 3二쇱뿉뒗 룊洹 42 mm, 洹몃━怨 4二 썑뿉뒗 룊洹 58 mm濡 굹궗떎. 蹂 떎뿕뿉꽌뒗 吏묐씫쓽 삎깭瑜 愿李고븯湲 쐞빐 SDA, PDA 諛곗뿉 젒醫낇븯뿬 諛곗뼇븳 寃곌낵 몢 諛곗媛꾩쓽 吏묐씫 냼寃ш낵 쁽誘멸꼍 냼寃ъ 겙 李⑥씠媛 뾾뿀떎. 吏묐씫쓽 삎깭瑜 蹂대㈃ 以묒븰遺뿉꽌 떆옉릺뒗 援듭 諛⑹궗긽쓽 二쇰쫫씠 蹂댁씠뒗 T. album삎씠 17 (85%)洹좎<, 吏묐씫쓽 쟾諛섏뿉 嫄몄퀜 誘몄꽭 二쇰쫫씠 엳뒗 T. ochraceum 삎씠 2 (10%)洹좎<, 꽦옣냽룄媛 鍮좊Ⅴ怨 二쇰쫫씠 뾾쑝硫 씛깋쓽 넑꽭紐⑥뼇 T. discoides 삎씠 1 (5%)洹좎<濡 굹궗떎.

씠 벑(Lee et al., 1991) 냼뿉꽌 遺꾨━븳 T. verrucosum 珥 56洹좎<뿉꽌 T. album 삎씠 28 (50%)洹좎<, T. ochraceum 삎씠 16 (25.6)洹좎<, 洹몃━怨 T. discoides 12 (21.4%)洹좎<濡 굹궗湲곗뿉 蹂 떎뿕怨 遺꾨━쑉뿉꽌 李⑥씠媛 엳쓬쓣 솗씤븯떎(Lee et al., 1991).

理 벑怨 씠 벑쓽 T. verrucosum쓽 쁽誘멸꼍 냼寃ъ 遺遺꾩쓽 끉臾몄뿉꽌 洹좎궗 썑留됲룷옄媛 깮꽦맖쓣 蹂닿퀬븯쑝굹 遺꾩깮옄 냼遺꾩깮옄쓽 깮꽦 愿李고븷 닔 뾾뿀떎怨 븯떎(Lee et al., 1991; Choe et al., 2006). 씠뿉 蹂 떎뿕뿉꽌룄 SDA 諛 PDA 諛곗뿉 젒醫낇븯뿬 諛곗뼇맂 吏묐씫쓣 Scotch tape method Slide culture濡 愿李고븳 寃곌낵 遺꾩깮옄 냼遺꾩깮옄쓽 깮꽦 愿李고븷 닔 뾾뿀怨 洹좎궗 T. verrucosum 룞젙뿉 以묒슂븳 냼寃ъ씤 궗뒳紐⑥뼇쓽 썑留됲룷옄뒗 T. album 삎뿉꽌留 蹂 닔 엳怨 떎瑜 삎뿉꽌뒗 洹좎궗 걹뿉 떒씪꽦 썑留됲룷옄留 蹂 닔 엳뿀떎.

T. verrucosum뿉 븳 紐⑤컻泥쒓났 떆뿕 留롮 븡쑝硫 씠 벑 냼뿉꽌 遺꾨━븳 3洹좎<瑜 떎뿕븳 寃곌낵 紐⑤몢 쓬꽦씠뿀떎(Lee et al., 1991). 源 벑 궗엺뿉寃 遺꾨━맂 30洹좎<瑜 紐⑤컻泥쒓났 寃궗瑜 떎떆븳 寃곌낵 30洹좎< 紐⑤몢媛 뼇꽦씠뿀怨 urease test뿉꽌룄 11洹좎<媛 뼇꽦씠뿀떎(Kim et al., 1992).

蹂 뿰援ъ쭊뿉 쓽븳 紐⑤컻泥쒓났 寃궗뒗 紐⑤컻泥쒓났 뼇꽦씠 3 (15%)洹좎<씠硫 씠 以 2洹좎<뒗 T. ochraceum 삎씠怨 1洹좎<뒗 T. discoides濡 議곗궗릺뿀떎. Urease 떆뿕뿉꽌 20洹좎<뒗 紐⑤몢 쓬꽦쑝濡 議곗궗릺뿀떎.

씠 뿰援ъ뿉꽌 룞臾쇱튇솕꽦씤 T. verrucosum씠 룞臾쇨낵 궗엺뿉꽌 遺꾨━릺뿀쓣 븣 紐⑤컻泥쒓났 寃궗 urease 떆뿕 寃곌낵媛 떎냼 李⑥씠媛 엳쓬쓣 솗씤븯湲곗뿉 씠 뿰援щ뒗 뼢썑 룞臾쇱튇솕꽦 吏꾧퇏씤 T. verrucosum쓽 蹂솕異붿씠뿉 湲곗큹옄猷뚭 맆 寃껋쑝濡 깮媛곷맂떎.

씠 끉臾몄뿉꽌 옄뒗 媛뺤썝룄 슒꽦쓽 븳슦 냽媛뿉꽌 냼 踰꾩쭚씠 엳뒗 븳슦뿉꽌 遺꾨━븳 T. verrucosum쓽 吏꾧퇏븰쟻 꽦긽쓣 愿李고븯떎. 냼뿉꽌 遺꾨━븳 20洹좎<쓽 T. verrucosum瑜 SDA, PDA 諛곗뿉 諛곗뼇븯뿬, 洹좎<쓽 듅吏뺤쓣 쑁븞쟻쑝濡 吏묐씫쓣 愿李고븯怨, 쁽誘멸꼍 냼寃, 紐⑤컻泥쒓났 떆뿕, urease 떆뿕쓣 넻빐 솗씤븯떎.

37℃뿉꽌 SDA 룊뙋諛곗뿉 諛곗뼇븯뿬 愿李고븳 寃곌낵 吏묐씫쓽 겕湲곕뒗 1二 룊洹 5 mm, 2二쇱뿉뒗 룊洹 33 mm, 3二쇱뿉뒗 룊洹 42 mm, 洹몃━怨 4二 썑뿉뒗 룊洹 58 mm濡 굹궗떎. 吏묐씫쓽 삎깭瑜 궡렣蹂대㈃, 以묒븰遺뿉꽌 떆옉릺뒗 援듭 諛⑹궗긽쓽 二쇰쫫쓣 蹂댁씠뒗 T. album 삎씠 17洹좎<, 吏묐씫쓽 쟾諛섏뿉 嫄몄퀜 誘몄꽭 二쇰쫫씠 엳뒗 T. ochraceum 삎씠 2洹좎<, 꽦옣냽룄媛 鍮좊Ⅴ怨 二쇰쫫씠 뾾쑝硫 씛깋쓽 넑꽭紐⑥뼇 T. discoides삎씠 1洹좎<媛 遺꾨━릺뿀떎.

SDA 諛 PDA 諛곗뿉 젒醫낇븯뿬 諛곗뼇맂 吏묐씫쓣 쁽誘멸꼍쑝濡 愿李고븳 寃곌낵 T. verrucosum 洹좎<뿉꽌 遺꾩깮옄 냼遺꾩깮옄뒗 愿李곕릺吏 븡븯怨, 洹좎궗 썑留됲룷옄뒗 T. album삎뿉꽌留 蹂 닔 엳뿀떎.

T. verrucosum 20洹좎<瑜 긽쑝濡 紐⑤컻泥쒓났 寃궗 寃곌낵뒗 뼇꽦 3洹좎<, urease 떆뿕뿉꽌뒗 20二 紐⑤몢뿉꽌 쓬꽦쑝濡 굹궗떎.

ACKNOWLEDGEMENT

None.

CONFLICT OF INTEREST

The authors have no conflict of interest to declare.

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