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The Antioxidative Effect of Eclipta prostrata L. Extract on Cultured NIH3T3 Fibroblasts Injured by Manganese-Induced Cytotoxicity
Biomed Sci Letters 2018;24:357-364
Published online December 31, 2018;
© 2018 The Korean Society For Biomedical Laboratory Sciences.

Sang-Hee Lee1,*, In-Ju Jung2,* and Hyesook Jang3,†,*

1Sanbon Hospital, School of Medicine, Wonkwang University, Gunpo 15865, Korea
2Department of Cosmetology, Dongshin University, Naju 58245, Korea
3Department of Nursing, Jeonju University, Jeonju-city 55069, Korea
Correspondence to: *Professor.
Hyesook Jang. Department of Nursing, Jeonju University, 303 Cheonjam-ro Wansan-gu, Jeonju-city 55069, Korea.
Tel: +82-63-220-4698, Fax: +82-63-220-2054, e-mail:
Received November 7, 2018; Revised December 4, 2018; Accepted December 13, 2018.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Manganese (Mn) is used as main materials in various chemical processes of industry, but it suggested that Mn brings about its toxicant by fume or dust through respiratory system and skin barrier. Mn toxicant induces the loss of mental health and life quality by cerebrovascular and skin diseases. Nevertheless, it lefts much unknown on the mechanism and the effectively therapeutic methods about Mn toxicant. Therefore, this study was evaluated the cytotoxicity induced by manganese dioxide (MnO2) in cultured NIH3T3 fibroblasts, and also, the correlation between MnO2-induced cytotoxicity and oxidative stress was examined. While, the effect of Eclipta prostrata L. (EP) extract belong to Compositae was assessed against MnO2-induced cytotoxicity in the view of antioxidative effect for searching the natural resources mitigating or preventing the MnO2-induced cytotoxicity. In this study, MnO2-induced cytotoxicity was revealed as mid-toxic by Borenfreud and Puerner's toxic criteria, and catalase (CAT), an antioxidant prevented MnO2-induced cytotoxicity by the remarkable increase of cell viability in these cultures. While, in the protective effect of EP extract on MnO2-induced cytotoxicity, EP extract effectively prevented the cytotoxicity induced by MnO2 via antioxidative effects such as xanthine oxidase (XO) inhibitory ability and DPPH-radical scavenging ability. From the above results, EP extract showed the effective prevention against MnO2-induced cytotoxicity correlated with oxidative stress by antioxidative effects. Conclusively, this study may be useful to research or development the alternatively therapeutic agent from natural resources like EP extract for the treatment of diseases resulted in oxidative stress.
Keywords : Toxicant, Manganese oxide, Alternative therapeutic agent, Antioxidative effect