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Anti-Oxidative and Anti-Neuroinflammatory Effect of Ethanol Extracts from Walnuts's (Juglans regia L.) Shell
Biomed Sci Letters 2018;24:365-371
Published online December 31, 2018;  https://doi.org/10.15616/BSL.2018.24.4.365
© 2018 The Korean Society For Biomedical Laboratory Sciences.

Hyun Kang†,*

Department of Medical Laboratory Science, College of Health Science, Dankook University, Cheonan-si, Chungnam 31116, Korea
Correspondence to: *Professor.
Hyun Kang. Department of Medical Laboratory Science, College of Health Science, Dankook University, Cheonan-si, Chungnam 31116, Korea.
Tel: +82-41-550-3015, Fax: +82-41-559-7934, e-mail: hkang@dankook.ac.kr
Received November 11, 2018; Revised December 1, 2018; Accepted December 5, 2018.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
 Abstract
In this study, antioxidant and anti-neuroinflammatory of ethanol extracts from walnuts's (Juglans regia L.) shell were investigated in vitro. Radical-scavenging activities of the walnuts's shell ethanol extracts (WSE) were examined by using ABTS radicals and α,α-diphenyl-β-picrylhydrazyl (DPPH) radicals assay. In the ABTS and DPPH radical scavenging activity, RC50 of WSE were measured as 15.74 and 40.13 µg/mL, respectively. Also, to evaluate the anti-neuroinflammatory effects of WSE in lipopolysaccharide (LPS)-stimulated BV-2 microglial cells. The production of proinflammatory cytokines NO were examined by LPS in BV-2 cell. BV-2 cells activated with LPS were treated with various doses (10, 25, 50, 100 µg/mL) of WSE. Supernatants were analyzed for the production of NO using Griess reagent. WSE up to 10 µg/mL still required to inhibit NO induced by LPS. These results showed that walnuts's (Juglans regia L.) shell can be used as an easily accessible source of natural anti-neuroinflammatory and natural antioxidants.
Keywords : Walnuts, Anti-inflammatory activity, ABTS, Microglial cells, NO