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Effect of Ethanol Extracts from Defatted Perilla frutescens on LPS-induced Inflammation in Mouse BV2 Microglial Cells
Biomed Sci Letters 2018;24:398-404
Published online December 31, 2018;  https://doi.org/10.15616/BSL.2018.24.4.398
© 2018 The Korean Society For Biomedical Laboratory Sciences.

Sung-Gyu Lee* and Hyun Kang†,*

Department of Medical Laboratory Science, College of Health Science, Dankook University, Cheonan-si, Chungnam 31116, Korea
Correspondence to: *Professor.
Hyun Kang. Department of Medical Laboratory Science, College of Health Science, Dankook University, Cheonan-si, Chungnam 31116, Korea.
Tel: +82-41-550-3015, Fax: +82-41-559-7934, e-mail: hkang@dankook.ac.kr
Received October 13, 2018; Revised December 10, 2018; Accepted December 13, 2018.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
 Abstract
To evaluate the antioxidant and anti-neuroinflammatory effects of defatted Perilla frutescens extract (DPE) in lipopolysaccharide (LPS)-stimulated BV-2 microglial cells. Cell viabilities were estimated by MTT assay. LPS-stimulated BV-2 microglia were used to study the expression and production of inflammatory mediators such as nitric oxide (NO), inducible NO synthase (iNOS), Cyclooxygenase-2 (COX-2), and prostaglandin E2 (PGE2). Pretreatment with DPE prior to LPS treatment significantly inhibited excessive production of NO (10, 25, 50, 75, and 100 µg/mL) in a dose-dependent manner, and was associated with down regulation of expression of iNOS and COX-2. DPE also suppressed the LPS-induced increase in PGE2 level (10, 25, 50, 75, and 100 µg/mL) in BV-2 cells. Therefore, DPE can be considered as a useful therapeutic and preventive approach for the treatment of several neurodegenerative diseases.
Keywords : Defatted Perilla frutescens, Anti-inflammatory activity, Microglial cells, INOS, COX-2