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A Comparison of Genospecies of Clinical Isolates in the Acinetobacter spp. Complex Obtained from Hospitalized Patients in Busan, Korea
Biomed Sci Letters 2019;25:40-53
Published online March 31, 2019;
© 2019 The Korean Society For Biomedical Laboratory Sciences.

Gyu-Nam Park1,* , Hye-Sook Kang2,** , Hye-Ran Kim3,*** , Bo-Kyung Jung1,*, Do-Hee Kim4,* and Kyung-Soo Chang1,,***

1Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan, Busan 46252, Korea 2Department of Laboratory Medicine, Maryknoll Medical Center, Busan 48972, Korea 3Department of Clinical Laboratory Science, College of Health and Therapy, Daegu Haany University, Gyeongsangbuk-Do 38610, Korea 4Department of Laboratory Medicine, Busan Veterans Hospital, Busan 46996, Korea
Correspondence to: Kyung-Soo Chang. Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan, Busan 46252, Korea.
Tel: +82-51-510-0565, Fax: +82-51-510-0568, e-mail:
Received December 18, 2018; Revised January 10, 2019; Accepted January 14, 2019.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Of the Acinetobacter spp., A. baumannii (genospecies 2) is the most clinically significant in terms of hospital-acquired infections worldwide. It is difficult to perform Acinetobacter-related taxonomy using phenotypic characteristics and routine laboratory methods owing to clusters of closely related species. The ability to accurately identify Acinetobacter spp. is clinically important because antimicrobial susceptibility and clinical relevance differs significantly among the different genospecies. Based on the medical importance of pathogenic Acinetobacter spp., the distribution and characterization of Acinetobacter spp. isolates from 123 clinical samples was determined in the current study using four typically applied bacterial identification methods; partial rpoB gene sequencing, amplified rRNA gene restriction analysis (ARDRA) of the intergenic transcribed spacer (ITS) region of the 16~23S rRNA, the VITEK® 2 system (an automated microbial identification system) and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). A. baumannii isolates (74.8%, 92/123) were the most common species, A. nosocomialis (10.6%, 13/123) and A. pittii isolates (7.5%, 9/123) were second and third most common strains of the A. calcoaceticus-A. baumannii (ACB) complex, respectively. A. soli (5.0%, 6/123) was the most common species of the non-ACB complex. RpoB gene sequencing and ARDRA of the ITS region were demonstrated to lead to more accurate species identification than the other methods of analysis used in this study. These results suggest that the use of rpoB genotyping and ARDRA of the ITS region is useful for the species-level identification of Acinetobacter isolates.
Keywords : Acinetobacter spp., Genomic species, RpoB genotyping, ARDRA of the ITS region, MALDI-TOF MS, Colony morphology