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Expression of Fas and TNFR1 in the Luteal Cell Types Isolated from the Ovarian Corpus Luteum
Biomed Sci Letters 2019;25:107-112
Published online March 31, 2019;  https://doi.org/10.15616/BSL.2019.25.1.107
© 2019 The Korean Society For Biomedical Laboratory Sciences.

Minseong Kim1,*, Sang-Hee Lee2,**, Seunghyung Lee1,*** and Gur-Yoo Kim1,,***

1College of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Korea 2Discipline of ICT, School of Technology, Environments and Design, University of Tasmania, Hobart, Tas7001, Australia
Correspondence to: Gur Yoo Kim. College of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Korea.
Tel: +82-33-250-8647, Fax: +82-33-259-5574, e-mail: gykim@kangwon.ac.kr
Received November 14, 2018; Revised March 12, 2019; Accepted March 22, 2019.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
 Abstract
The corpus luteum (CL) is composed to various cells, such as luteal steroidogenic cells (LSCs), luteal thecal steroidogenic cells (LTCs), luteal endothelial cells (LECs), fibroblast, immune cells and blood cells. The life span of CL is controlled by proliferation and apoptosis of luteal cells. Therefore, this study investigated apoptotic factors in luteal cells derived from bovine CL. The CL tissues were collected from bovine ovaries and luteal cells were isolated from middle phase CL. Then, LTCs and LECs were separated according to cellular morphology from LSCs. The expression of Bax, Bcl-2, Fas and tumor necrosis factor 1 receptor (TNF1R) mRNA and protein were analyzed using quantitative RT-PCR and western blot. Results show that, Bax and TNFR1 mRNA expression were significantly increased at late group than early and middle groups, otherwise Bcl-2 were significantly decreased at late group than early group (P<0.05). Fas mRNA expression were significantly decreased in middle group compared to early and late groups (P<0.05). In addition, Bax and Bcl-2 mRNA in LTCs was lower than LSCs, Fas mRNA was higher than LSCs. The Bcl-2 protein expression was lower at LTCs than LSCs, especially Fas protein in LTCs was significantly lower than LSCs and LECs (P<0.05). Otherwise, TNFR1 protein of LTCs were similar with LSCs but higher compared with LECs. In conclusion, we suggest that the results may help understanding of apoptosis ability in luteal cells according to cell type during CL regression of estrous cycle.
Keywords : Corpus luteum, Luteal steroidogenic cells, Luteal thecal steroidogenic cells, Apoptosis