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Modulation Effects on Acute Orofacial Inflammatory Pain in Rats by Curcuma longa L., Curcuma aromatica Salisb., Zingiber officinale Rosc. Extracts
Biomed Sci Letters 2019;25:247-255
Published online September 30, 2019;  https://doi.org/10.15616/BSL.2019.25.3.247
© 2019 The Korean Society For Biomedical Laboratory Sciences.

Hee-Jin Kim*, Ja-Hyung Choi*, Hye-Jin Kim**, Hyun-Soe Yoon** and Min-Kyung Lee†,**

Department of Biomedical Health Science, Dong-eui University, Busan 47340, Korea
Correspondence to: Min-Kyung Lee. Department of Biomedical Health Science, Dong-eui University, 176 Eongwangno(gayadong san24), Busanjin-gu, Busan 47340, Korea.
Tel: +82-51-890-4238, Fax: +82-0505-182-6878, e-mail: lmk849@deu.ac.kr
*Graduate student, **Professor.
Received August 5, 2019; Revised September 10, 2019; Accepted September 16, 2019.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
 Abstract
Curcuma longa L. (C.L), Curcuma aromatica Salisb. (C.A) and Zingiber officinale Rosc. (Z.O) of Zingiberaceae plants which are well known as effects of natural anti-oxidant, anti-cancer and anti-inflammatory. We examined that the Zingiberaceae plants are involved in development and modulation of orofacial pain in rats. Male, 7- to 8-week-old, Sprague-Dawley rats weighing 240~280 g were used in this study. Experiments were performed using acute pain model that was caused by the injection of 5% formalin into the right vibrissa pad. The number of scratching or rubbing to the injection site was recorded for 9 consecutive 5-minute intervals following injection of formalin. The experimental groups were acute orofacial inflammatory pain; control group (formalin, 5%), vehicle group (5% formalin after sodium carboxymethyl cellulose), single administration group, single mixed administration group, repeated administration group. The experiments were performed various concentrations of Zingiberaceae plants extract. Therefore, oral administration of C.L, C.A, and Z.O (p.o., concentrations of 12.5, 25 mg/mL) in orofacial inflammatory pain model substantially decrease the nociceptive behavior in a concentration dependent manner. And it tended to decrease at low concentration (12.5 mg/mL) of single mixed and repeated administration more than single administration. This result means that Zingiberaceae plants extract affects the modulation of acute orofacial inflammatory pain. Thus, Zingiberaceae plants extract may be a potential therapeutic treatment for orofacial inflammatory pain.
Keywords : Curcuma longa L., Curcuma aromatica Salisb., Zingiber officinale Rosc., Inflammatory, Orofacial pain
꽌 濡

泥쒖뿰臾쇱뿉 議댁옱븯뒗 뤃由ы럹솕빀臾(polyphenolics) 泥쒖뿰 깋냼濡쒖꽌 옄뿰怨꾩뿉 꼸由 議댁옱븯怨 엳쑝硫 二쇰줈 븸룷 諛 꽭룷留됱뿉꽌 쑀由ы삎, 뿉뒪뀒瑜댄삎 삉뒗 寃고빀삎쑝濡 議댁옱븳떎(Lee et al., 2011). 뤃由ы럹(polyphenols)뿉 議댁옱븯뒗 떎닔쓽 엳뱶濡앹떎湲(-OH)뒗 뿬윭 솕빀臾쇨낵 돺寃 寃고빀븯뒗 듅꽦쓣 媛吏怨 엳뼱 빆븫, 빆뿼 諛 빆궛솕 슚怨쇨 쎇뼱굹떎(Kim et al., 2012). 씠誘 꽑吏꾧컖援뿉꽌뒗 泥쒖뿰臾 궛뾽(natural products industry)뿉 븳 愿떖씠 넂븘 씠 遺꾩빞쓽 뿰援ш컻諛쒖뿉 뿭젏쓣 몢怨 媛곸쥌 泥쒖뿰臾쇱쓽 젣뭹솕 궛뾽솕뿉 뿭웾쓣 吏묒쨷븯怨 엳뒗 異붿꽭씠떎(Hwang and Park, 2015). 떎뀈깮 珥덈낯()뿉 냽븯뒗 깮媛뺢낵(Zingiberaceae) 떇臾쇰뱾뿉뒗 깮媛, 媛뺥솴, 슱湲덉씠 엳떎. 洹 以묒뿉꽌 깮媛(Zingiber officinale Roscoe) 뼢떊猷뚮줈 꽭怨꾩쟻쑝濡 꼸由 궗슜릺怨 엳쑝硫 깋 뼱몢슫 끂깋遺꽣 솴깋 삉뒗 遺됱깋쓣 씈怨, 깮媛 듅쑀쓽 옄洹뱀쟻씤 뼢 二쇱꽦遺꾩씤 gingerol, shogaol, zingerone 벑뿉 湲곗씤븯硫, 씠 쇅뿉룄 flavonoids, terpenoids 벑 떎뼇븳 깮由ы솢꽦 솕빀臾쇱씠 議댁옱븯뒗 寃껋쑝濡 蹂닿퀬릺怨 엳떎(Guon and Chung, 2016). 깮媛 異붿텧臾 以묒쓽 븯굹씤 6-gingerol cyclooxygenase-2 (COX-2) 솢꽦쓣 슚怨쇱쟻쑝濡 뼲젣븳떎怨 諛앺議뚯쑝硫 shogaol怨 gingerol씠 prostaglandin (PG) 諛 leukotriene 빀꽦 슚냼쓽 슚怨쇱쟻씤 뼲젣젣濡 蹂닿퀬릺뿀떎(Kim et al., 2008). 깮媛뺢낵 떇臾쇱뿉 냽븯뒗 슱湲(Curcuma longa L., turmeric) 쟾넻쟻쑝濡 븳빟옱, 뼢떊猷 諛 떇슜쑝濡 궗슜릺뼱 솕떎(Kim and Lee, 2014). 슱湲덉쓽 떎뼇븳 슚뒫쑝濡쒕뒗 媛꾩옣쓽 빐룆 珥됱쭊怨 떞利숈쓽 遺꾨퉬옉슜 諛 씠삁옉슜씠 쎇뼱굹떎. 뵲씪꽌 씠떞옉슜怨 諛⑺뼢꽑 嫄댁쐞빟, 移댄瑜댁꽦 솴떖빟, 떞꽍利, 湲됱꽦 媛꾩뿼빟 벑 援궡쇅뿉꽌 떎뼇븳 泥섎갑怨 젣빟쓽 썝猷뚮줈 닔슂媛 留롮쑝硫, 理쒓렐 슱湲덉쓽 깮由ы솢꽦 臾쇱쭏씤 curcuminoids쓽 빟由 슚怨쇨 븣젮吏硫댁꽌 쓽븰 遺꾩빞瑜 以묒떖쑝濡 留롮 뿰援ш 솢諛쒗엳 씠猷⑥뼱吏怨 엳뒗 떎젙씠떎(Kim et al., 2011). Curcuminoids뒗 꽭 媛吏쓽 솕빀臾쇰줈 援ъ꽦릺뼱 엳뒗뜲 curcumin, demethoxycurcumin (DMC) 諛 bisdemethoxycurcumin (BMC)씠硫 洹 以묒뿉꽌룄 솴깋 깋냼濡쒖뜥 媛곸쥌 빟由ъ쟻 솢꽦쓣 媛吏怨 엳뒗 curcumin 삤옒쟾遺꽣 쓽븰, 빟븰, 떇뭹븰 遺꾩빞 벑뿉꽌 슚뒫씠 엯利앸릺뿀쑝硫, 떎뼇븳 뿰援ш 蹂닿퀬릺뿀떎(Kim et al., 2017). 留덉留됱쑝濡, 깮媛뺢낵(Zingiberaceae)쓽 媛뺥솴 냽(Curcuma)쑝濡 遺꾨쪟릺뒗 떎뀈깮 떇臾 媛뺥솴(Anandakumar et al., 2014) 뿬윭 떎뿕쓣 넻븳 뿰援ъ뿉꽌 二쇱슂 꽦遺꾩씤 curcumin쓽 룞留κ꼍솕 뼲젣, 빆뿼利 諛 빆븫 슚怨, 빆궛솕 슚怨, 빆洹 슚怨 벑씠 洹쒕챸릺뿀떎(Yoon et al., 2006). 씠泥섎읆 깮媛뺢낵 떇臾쇱뿉 냽븯뒗 슱湲, 媛뺥솴, 깮媛뺤쓽 빟由ъ쟻 솢꽦 씠誘 엯利앸릺뿀쑝硫, 떎뼇븳 遺꾩빞뿉꽌 泥쒖뿰臾쇱뿉 愿젴맂 뿰援ш 솢諛쒗븯寃 씠猷⑥뼱吏怨 엳떎. 洹몃윭굹 븘吏곴퉴吏 깮媛뺢낵 떇臾쇰뱾씠 援ш컯 븞硫 쁺뿭뿉꽌 뿼利앹꽦 넻利앹쓽 議곗젅怨 愿젴맂 뿰援ш 쟾臾댄븳 떎젙씠誘濡 蹂 뿰援ъ뿉꽌 븞硫대 뿼利앹꽦 넻利 諛쒖깮怨 슱湲, 媛뺥솴, 깮媛 異붿텧臾쇱쓽 넻利 셿솕뿉 븳 슚怨쇰 븣븘蹂닿퀬옄 븳떎.

몢遺, 븞硫 諛 紐⑤뱺 援ш컯 궡 援ъ“臾쇱쓽 寃쎌“吏 諛 뿰議곗쭅怨 愿젴맂 援ш컯븞硫 넻利(orofacial pain) 移섏븘 吏덊솚쓣 鍮꾨’븯뿬 痢〓몢븯븙 愿젅 옣븷(temporomandibular disorders, TMD) 肉먮쭔 븘땲씪 궪李⑥떊寃쏀넻 媛숈 떊寃쎈퀝꽦 넻利앷퉴吏 떎뼇븳 吏덊솚뱾씠 쑀궗븳 삎깭쓽 룞넻쑝濡 諛쒗쁽릺誘濡 吏꾨떒씠 뼱졄떎(Choi et al., 2006). 援ш컯 쓬떇, 쓬猷 諛 怨듦린쓽 꽠痍⑤ 媛뒫븯寃 븯뒗 깮臾쇳븰쟻 援ъ“씠誘濡 씤泥댁쓽 諛쒕떖, 꽦옣 諛 쑀吏瑜 吏썝븯뒗뜲 븘슂븳 媛옣 湲곕낯쟻씤 슂냼씪怨 븷 닔 엳떎. 뵲씪꽌 援ш컯 궡 뿼利 遺쐞쓽 蹂묐 빆긽 씤泥 嫄닿컯뿉 빐濡怨 媛쒖씤쓣 援ъ꽦븯뒗 꽭룷쓽 紐⑤뱺 깮臾쇳븰쟻 솢룞쓣 넀긽떆궓떎(Moritani et al., 2018). 씠윭븳 뿼利앸컲쓳 깮泥댁쓽 빆긽꽦쓣 쑀吏븯湲 쐞븳 留ㅼ슦 븘닔쟻씠硫 쑀씡븳 諛섏쓳씠吏留 뿼利앸컲쓳 議곗젅뿉 떎뙣븯뿬 怨쇰텇鍮 맂 뿼利 留ㅺ컻 臾쇱쭏 꽭룷굹 議곗쭅쓽 愿댁궗 諛 湲곕뒫 옣븷瑜 씪쑝궎怨 솢꽦솕맂 硫댁뿭꽭룷쓽 깮議댁쓣 怨꾩냽 쑀吏떆耳 留뚯꽦 뿼利앸컲쓳쓽 썝씤씠 맂떎(Yoon et al., 2015). 넻利앹 議곗쭅 넀긽, 뿼利 諛쒖깮 벑怨 媛숈 넻利앹쓣 諛쒖깮떆궎뒗 옄洹뱀뿉 쓽빐꽌 넻利앹꽦 떊寃쎌쟾떖臾쇱쭏씠 쑀由щ릺怨 떊寃쎌쟾떖臾쇱쭏씠 꽭룷留됱쓽 닔슜湲곗뿉 옉슜븯뿬 씎遺꾩꽦 솢룞쟾쐞瑜 諛쒖깮븳떎(Bae et al., 2013). 씠윭븳 뿼利앸컲쓳씠 븞硫대뿉 諛쒖깮릺硫 븞硫대 뿼利앹꽦 넻利앹씠 쑀諛쒕맂떎. 씠윭븳 넻利앹쓽 議곗젅뿉 二쇰줈 留롮씠 궗슜릺怨 엳뒗 鍮꾩뒪뀒濡쒖씠뱶꽦 냼뿼吏꾪넻젣(Nonsteroidal anti-inflammatory drug; NSAIDs)뒗 솕븰쟻쑝濡 씠吏덉쟻씤 솕빀臾 援곗씠硫, 吏꾪넻(analgesics) 諛 빆뿼(anti-inflammatory), 빐뿴(antipyretic) 옉슜쓣 怨듯넻맂 듅吏뺤쑝濡 媛吏怨 엳떎. 洹몃윭굹 NSAIDs뒗 떎뼇븳 遺옉슜쓣 씪쑝궎뒗뜲 듅엳 쐞옣愿뿉 떎뼇븳 빀蹂묒쬆쓣 씪쑝궗 닔 엳떎. 臾댁쬆긽쓽 쐞 젏留 넀긽씠굹 냽 벐由, 냼솕遺덈웾쑝濡쒕꽣 깮紐낆쓣 쐞삊븯뒗 쐞異쒗삁씠굹 떗씠吏옣쓽 沅ㅼ뼇怨 愿젴씠 엳뼱(Song and Ha, 2009), 궗슜 떆 二쇱쓽瑜 븘슂濡 븳떎. 씤泥 吏덊솚 移섎즺젣쓽 寃쎌슦뿉뒗 泥쒖뿰臾쇱쓽 냼옱濡 븳 떊빟 諛 쓽빟뭹씠 異쒖떆릺뼱 엫긽 移섎즺뿉 쟻슜릺怨 엳쑝굹 援ш컯 吏덊솚 諛 移섍낵 愿젴 吏덊솚 移섎즺뿉 씠슜릺怨 엳뒗 쓽빟뭹 遺遺꾩씠 빀꽦 빟젣濡 遺옉슜씠 利앷븯뒗 異붿꽭씠怨, 移섏쓽븰 遺꾩빞뿉꽌뒗 씠뿉 븳 뿰援ш 誘몃명븯떎(Lee et al., 2011).

뵲씪꽌 蹂 뿰援ъ뿉꽌뒗 룷瑜대쭚由곗쑝濡 쑀룄븳 븞硫대 뿼利앹꽦 넻利앹쓣 떎뿕 紐⑤뜽뿉寃 쟻슜븯뿬 슱湲, 媛뺥솴, 깮媛 異붿텧臾쇱쓽 二쇱엯뿉 쓽븳 넻利 뻾쐞 諛섏쓳 議곗젅 슚怨쇰 솗씤븯怨좎옄 븯떎.

옱猷 諛 諛⑸쾿

떎뿕옱猷

떎뿕룞臾쇱 7~8二 졊쓽 Sprague-Dawley 怨 닔而 씛伊(220~260 g)瑜 슚李 궗씠뼵뒪(援)뿉꽌 怨듦툒諛쏆븘 궗슜븯떎. 23~25°C쓽 씪젙븳 떎궡 삩룄 12떆媛 二/빞 닚솚 二쇨린븯뿬 솚寃쎌쓣 쑀吏븯쑝硫, 臾쇨퀬 궗猷뚮뒗 옄쑀濡쒖씠 怨듦툒븯떎. 뻾룞쟻씤 뼲븬 벑뿉 쓽븳 떎뿕 쟾 뒪듃젅뒪瑜 媛뒫븳 理쒖냼솕븯쑝硫, 蹂 뿰援щ뒗 쓽떇씠 엳뒗 룞臾쇱쓽 떎뿕뿉 愿븳 넻利 뿰援 븰쉶쓽 쑄由ъ쟻 洹쒖젙쓣 以닔븯怨 룞쓽븰援 룞臾쇱쑄由 쐞썝쉶쓽 듅씤(R2018-012)쓣 뼸 썑 닔뻾븯떎.

떆빟젣議

슱湲(援궛)怨 媛뺥솴(씤룄궛) 遺꾨쭚 (二)泥슫 쑀넻뿉꽌 援ъ엯븯쑝硫, 깮媛(援궛) 遺꾨쭚 (二)쁽 떇뭹뿉꽌 援ъ엯븯뿬 궗슜븯떎. 5% 룷瑜대쭚由 二쇱엯援곌낵 슜留ㅺ뎔(Vehicle [veh.], 5% sodium carboxymethyl cellulose [CMC-Na]), 슱湲 異붿텧臾 닾뿬援, 媛뺥솴 異붿텧臾 닾뿬援, 깮媛 異붿텧臾 닾뿬援곗쑝濡 떎뿕 洹몃9 援щ텇븯怨 5% CMC-Na뿉 媛곴컖 씗꽍븯뿬 궗슜븯떎. 媛 빟臾쇱쓽 닾뿬뒗 떒쉶 諛섎났(1씪 1쉶, 3씪媛 留ㅼ씪 1쉶) 洹몃━怨 떒쉶 샎빀(슱湲, 媛뺥솴, 깮媛) 닾뿬援곗쑝濡 援щ텇븯떎.

븞硫대 뿼利앹꽦 넻利앹쓽 諛쒗쁽 諛 슱湲, 媛뺥솴, 깮媛 異붿텧臾 슚怨쇱쓽 룊媛

옯뱶슜 oral zonde (∅1.8(15 gauge)*80 mm)瑜 씠슜븯뿬 슱湲, 媛뺥솴, 깮媛 異붿텧臾쇱쓣 媛곴컖 1 mL쓽 슜웾쑝濡 寃쎄뎄 닾뿬븯怨, 빟臾쇱쓽 二쇱엯 룷瑜대쭚由곗뿉 쓽븳 넻利 쑀룄 30遺 쟾뿉 떆뻾븯떎. 떎뿕룞臾쇱쓣 넻利 諛섏쓳 룊媛 쟾뿉 떎뿕슜 뵆씪뒪떛 넻뿉꽌 30遺 씠긽 쟻쓳떆耳곗쑝硫, 룷瑜대쭚由 諛섏쓳 씠 뿰援ъ뿉꽌 궗슜맂 諛⑸쾿怨 룞씪븯寃 닔뻾븯떎(Park et al., 2011). 떎뿕룞臾(n=6)쓽 삤瑜몄そ 닔뿼遺 뵾븯濡 씤뒓由 二쇱궗湲(0.25 mm(31 gauge)*8 mm)瑜 씠슜븯뿬 5% 룷瑜대쭚由(50 μL)쓣 二쇱엯븯쑝硫 빟臾쇱씠 二쇱엯맂 븞硫대 遺쐞瑜 臾몄瑜닿굅굹 湲곷뒗 뻾쐞瑜 넻利 吏몴濡 媛꾩<븯떎. 二쇱엯맂 吏곹썑遺꽣 5遺 떒쐞濡 늻쟻븯뿬 珥 45遺꾧컙 湲곕줉븯쑝硫, 1李 諛섏쓳(0~10遺, 1st phase)怨 2李 諛섏쓳(11~45遺, 2nd phase)쑝濡 援щ텇븯뿬 룊媛븯떎.

넻怨 遺꾩꽍

떎뿕 寃곌낵쓽 넻怨 遺꾩꽍 IBM SPSS statistic ver. 22 (IBM Co., Armonk, NY, USA)쓣 궗슜븯뿬 떎以 洹몃9뿉꽌 諛섎났 痢≪젙 옄猷뚯쓽 씪썝 諛곗튂 遺꾩궛쑝濡 遺꾩꽍븯쑝硫 궗썑遺꾩꽍 LSD post-hoc test瑜 씠슜븯떎. 넻怨꾩쟻씤 鍮꾧탳瑜 쐞빐 쑀쓽닔以 P<0.05濡 꽕젙븯떎. 寃곌낵뒗 룊洹 ± 몴以 삤李(SEM)濡 몴떆븯떎.

寃 怨

븞硫대 뿼利앹꽦 넻利 뻾쐞 諛섏쓳 議곗젅뿉 슱湲 異붿텧臾쇱쓽 슚怨

떎뿕룞臾쇱쓽 삤瑜몄そ 닔뿼遺 뵾븯濡 5% 룷瑜대쭚由(50 μL)쓣 닾뿬븯뿬 쑀룄븳 븞硫대 뿼利앹꽦 넻利앹뿉꽌 슱湲덉쓽 넻利 뻾쐞 諛섏쓳 議곗젅 슚怨쇰뒗 떎쓬怨 媛숈씠 굹궗떎(Fig. 1). 븞硫대 遺쐞쓽 1李 넻利 뻾쐞 諛섏쓳 룷瑜대쭚由곌뎔怨 슜留(CMC-Na)援곗뿉꽌 媛 29.00 ± 16.20, 15.67 ± 7.08쉶濡 굹궗怨, 슱湲 닾뿬援곗뿉꽌뒗 냽룄(12.5, 25 mg/mL)뿉 뵲씪 媛 15.83 ± 7.42, 20.67 ± 10.46쉶濡 굹굹 議곌뎔怨 떎뿕援 媛꾩쓽 李⑥씠媛 뾾뿀떎. 넻利앺뻾쐞 2李 諛섏쓳쓽 寃곌낵뿉꽌뒗 룷瑜대쭚由곌뎔怨 슜留ㅺ뎔 媛 329.00 ± 12.86, 349.33 ± 46.15쉶濡 넻利 뻾쐞 諛섏쓳씠 넂寃 굹궗쑝硫, 12.5 mg/mL쓽 슱湲 닾뿬援곗 236.83 ± 33.92쉶濡 議곌뎔怨 鍮꾧탳븯뿬 넻利 뻾쐞 諛섏쓳씠 媛먯냼맖쓣 굹궗떎. 25 mg/mL쓽 냽룄뿉꽌뒗 162.00 ± 27.68쉶濡 굹궡뼱 議곌뎔 諛 12.5 mg/mL쓽 냽룄 鍮꾧탳 떆 넻利 뻾쐞 諛섏쓳씠 냽룄 쓽議댁쟻쑝濡 媛먯냼맖쓣 솗씤븯떎(P<0.05).

Fig. 1.

Effects of C.L on nociceptive behavior at facial area. Following administration of C.L, the nociceptive responses were reduced in 2nd phase in a dose-dependent manner. There were 6 animals in each group. *P<0.05, Formalin vs C.L 12.5, 25 mg/mL + F.

C.L: Curcuma longa L.



떆媛꾩쓽 寃쎄낵뿉 뵲瑜 넻利 뻾쐞 諛섏쓳쓽 蹂솕뒗 떎쓬怨 媛숇떎(Fig. 2). 룷瑜대쭚由 二쇱엯쑝濡 쑀룄맂 넻利 뻾쐞 諛섏쓳 15遺 씠썑遺꽣 利앷븯뿬 25遺꾩뿉 媛옣 넂寃 굹궗쑝硫 40遺꾧퉴吏 넻利앹씠 吏냽릺떎媛 45遺꾩뿉 媛먯냼븯떎. 12.5 mg/mL쓽 슱湲덉쓽 寃쎄뎄 닾뿬뒗 룷瑜대쭚由 二쇱엯 썑 30~35遺, 25 mg/mL뒗 20~30遺꾩뿉 룷瑜대쭚由곗뿉 쓽빐 쑀룄맂 넻利 뻾쐞 諛섏쓳쓣 쑀쓽븯寃 媛먯냼떆耳곕떎.

Fig. 2.

Changes in nociceptive responses at facial area following administration of C.L. Oral administration of C.L, significantly reduced the nociceptive responses 20~30 mim after induction of pain. There were 6 animals in each group. *P<0.05, Formalin vs C.L 12.5, 25 mg/mL + F.

C.L: Curcuma longa L.



븞硫대 뿼利앹꽦 넻利 뻾쐞 諛섏쓳 議곗젅뿉 媛뺥솴 異붿텧臾쇱쓽 슚怨

떎뿕룞臾쇱쓽 삤瑜몄そ 닔뿼遺 뵾븯濡 5% 룷瑜대쭚由(50 μL)쓣 닾뿬븯뿬 쑀룄븳 븞硫대 뿼利앹꽦 넻利앹뿉꽌 媛뺥솴쓽 넻利 뻾쐞 諛섏쓳 議곗젅 슚怨쇰뒗 떎쓬怨 媛숈씠 굹궗떎(Fig. 3). 븞硫대 遺쐞쓽 1李 넻利 뻾쐞 諛섏쓳 룷瑜대쭚由곌뎔怨 슜留(CMC-Na)援곗뿉꽌 媛 63.33 ± 21.30, 17.60 ± 8.35쉶濡 굹궗怨, 媛뺥솴 닾뿬援곗뿉꽌뒗 냽룄(12.5, 25 mg/mL)뿉 뵲씪 媛 19.17 ± 7.31, 10.83 ± 3.88쉶濡 굹굹 議곌뎔怨 떎뿕援 媛꾩쓽 李⑥씠媛 뾾뿀떎. 넻利 뻾쐞 2李 諛섏쓳쓽 寃곌낵뿉꽌뒗 룷瑜대쭚由 援곌낵 슜留ㅺ뎔 媛 350.10 ± 40.54, 371.20 ± 49.78쉶濡 넻利 뻾쐞 諛섏쓳씠 넂寃 굹궗쑝硫, 12.5 mg/mL쓽 媛뺥솴 닾뿬援곗 249.33 ± 34.08쉶濡 議곌뎔怨 鍮꾧탳븯뿬 넻利 뻾쐞 諛섏쓳씠 媛먯냼맖쓣 굹깉떎. 25 mg/mL쓽 냽룄뿉꽌뒗 175.00 ± 17.45쑝濡 굹궡뼱 議곌뎔 諛 12.5 mg/mL쓽 냽룄 鍮꾧탳 떆 넻利 뻾쐞 諛섏쓳씠 냽룄 쓽議댁쟻쑝濡 媛먯냼맖쓣 솗씤븯떎(P<0.05).

Fig. 3.

Effects of C.A on nociceptive behavior at facial area. Following administration of C.A, the nociceptive responses were reduced in 2nd phase in a dose-dependent manner. There were 6 animals in each group. *P<0.05, Formalin vs C.A 12.5, 25 mg/mL + F.

>C.A: Curcuma aroma tica Salisb.



떆媛꾩쓽 寃쎄낵뿉 뵲瑜 넻利 뻾쐞 諛섏쓳쓽 蹂솕뒗 떎쓬怨 媛숇떎(Fig. 4). 룷瑜대쭚由곗쓽 二쇱엯쑝濡 쑀룄맂 넻利 뻾쐞 諛섏쓳 15遺 씠썑遺꽣 利앷븯뿬 25遺꾩뿉 媛옣 넂寃 굹궗쑝硫 40遺꾧퉴吏 넻利앹씠 吏냽릺떎媛 45遺꾩뿉 媛먯냼븯떎. 12.5 mg/mL쓽 媛뺥솴쓽 寃쎄뎄 닾뿬뒗 룷瑜대쭚由 二쇱엯 썑 25~35遺, 25 mg/mL뒗 20~30遺꾩뿉 룷瑜대쭚由곗뿉 쓽빐 쑀룄맂 넻利 뻾쐞 諛섏쓳쓣 쑀쓽븯寃 媛먯냼떆耳곕떎.

Fig. 4.

Changes in nociceptive responses at facial area following administration of C.A. Oral administration of C.A, significantly reduced the nociceptive responses 20~30 mim after induction of pain. There were 6 animals in each group. *P<0.05, Formalin vs C.A 12.5, 25 mg/mL + F.

C.A: Curcuma aroma tica Salisb.



븞硫대 뿼利앹꽦 넻利 뻾쐞 諛섏쓳 議곗젅뿉 깮媛 異붿텧臾쇱쓽 슚怨

떎뿕룞臾쇱쓽 삤瑜몄そ 닔뿼遺 뵾븯濡 5% 룷瑜대쭚由(50 μL)쓣 닾뿬븯뿬 쑀룄븳 븞硫대 뿼利앹꽦 넻利앹뿉꽌 깮媛뺤쓽 넻利 뻾쐞 諛섏쓳 議곗젅 슚怨쇰뒗 떎쓬怨 媛숈씠 굹궗떎(Fig. 5). 븞硫대 遺쐞쓽 1李 넻利 뻾쐞 諛섏쓳 룷瑜대쭚由곌뎔怨 슜留 (CMC-Na)援곗뿉꽌 媛 19.17 ± 8.01, 22.00± 9.16쉶濡 굹궗怨, 깮媛 닾뿬援곗뿉꽌뒗 냽룄(12.5, 25 mg/mL)뿉 뵲씪 媛 17.83 ± 6.49, 7.67 ± 3.27쉶濡 굹굹 議곌뎔怨 떎뿕援 媛꾩쓽 李⑥씠媛 뾾뿀떎. 넻利 뻾쐞 2李 諛섏쓳쓽 寃곌낵뿉꽌뒗 룷瑜대쭚由곌뎔怨 슜留ㅺ뎔 媛 370.00 ± 35.94, 357.00 ± 60.63쉶濡 넻利 뻾쐞 諛섏쓳씠 넂寃 굹궗쑝硫, 12.5 mg/mL쓽 媛뺥솴 닾뿬援곗 248.50 ± 20.78쉶濡 議곌뎔怨 鍮꾧탳븯뿬 넻利 뻾쐞 諛섏쓳씠 媛먯냼맖쓣 굹깉떎. 25 mg/mL쓽 냽룄뿉꽌뒗 165.00 ± 30.07쉶濡 굹궡뼱 議곌뎔 諛 12.5 mg/mL쓽 냽룄 鍮꾧탳 떆 넻利 뻾쐞 諛섏쓳씠 냽룄 쓽議댁쟻쑝濡 媛먯냼맖쓣 솗씤븯떎(P<0.05).

Fig. 5.

Effects of Z.O on nociceptive behavior at facial area. Following administration of Z.O, the nociceptive responses were reduced in 2nd phase in a dose-dependent manner. There were 6 animals in each group. *P<0.05, Formalin vs Z.O 12.5, 25 mg/mL + F.

Z.O: Zingiber officinale Rosc.



떆媛꾩쓽 寃쎄낵뿉 뵲瑜 넻利 뻾쐞 諛섏쓳쓽 蹂솕뒗 떎쓬怨 媛숇떎(Fig. 6). 룷瑜대쭚由곗쓽 二쇱엯쑝濡 쑀룄맂 넻利 뻾쐞 諛섏쓳 15遺 씠썑遺꽣 利앷븯뿬 25遺꾩뿉 媛옣 넂寃 굹궗쑝硫 40遺꾧퉴吏 넻利앹씠 吏냽릺떎媛 45遺꾩뿉 媛먯냼븯떎. 12.5 mg/mL쓽 깮媛뺤쓽 寃쎄뎄 닾뿬뒗 룷瑜대쭚由 二쇱엯 썑 30~35遺, 25 mg/mL뒗 20~30遺꾩뿉 룷瑜대쭚由곗뿉 쓽빐 쑀룄맂 넻利 뻾쐞 諛섏쓳쓣 쑀쓽븯寃 媛먯냼떆耳곕떎.

Fig. 6.

Changes in nociceptive responses at facial area following administration of Z.O. Oral administration of Z.O, significantly reduced the nociceptive responses 20~30 mim after induction of pain. There were 6 animals in each group. *P<0.05, Formalin vs Z.O 12.5, 25 mg/mL + F.

Z.O: Zingiber officinale Rosc.



븞硫대 뿼利앹꽦 넻利 뻾쐞 諛섏쓳 議곗젅뿉 슱湲, 媛뺥솴, 깮媛 異붿텧臾 샎빀 닾뿬쓽 슚怨

떎뿕룞臾쇱쓽 삤瑜몄そ 닔뿼遺 뵾븯濡 5% 룷瑜대쭚由(50 μL)쓣 닾뿬븯뿬 쑀룄븳 븞硫대 뿼利앹꽦 넻利앹뿉꽌 슱湲, 媛뺥솴, 깮媛 떒쉶 샎빀 닾뿬쓽 넻利 뻾쐞 諛섏쓳 議곗젅 슚怨쇰뒗 떎쓬怨 媛숈씠 굹궗떎(Fig. 7). 븞硫대 遺쐞쓽 1李 넻利 뻾쐞 諛섏쓳 룷瑜대쭚由곌뎔怨 슜留(CMC-Na)援곗뿉꽌 媛 19.12 ± 7.29, 8.67± 3.67쉶濡 굹궗怨, 슱湲, 媛뺥솴, 깮媛 12.5 mg/mL 닾뿬援곗뿉꽌뒗 媛 15.83 ± 7.42, 19.17 ± 7.31, 17.83 ± 6.49쉶濡 굹궗떎. 슱湲, 媛뺥솴, 깮媛 12.5 mg/mL쓣 샎빀븯뿬 닾뿬븳 援곗뿉꽌뒗 9.33 ± 6.10쉶濡 굹굹 議곌뎔怨 떎뿕援 媛꾩쓽 겙 李⑥씠媛 뾾뿀떎. 넻利 뻾쐞 2李 諛섏쓳쓽 寃곌낵뿉꽌뒗 룷瑜대쭚由곌뎔怨 슜留ㅺ뎔 媛 332.67 ± 35.11, 344.50 ± 21.43쉶濡 넻利 뻾쐞 諛섏쓳씠 넂寃 굹궗쑝硫, 12.5 mg/mL쓽 슱湲, 媛뺥솴, 깮媛 닾뿬援곗 媛 236.83 ± 33.93, 249.33 ± 34.08, 248.50 ± 20.78쉶濡 굹굹 議곌뎔怨 鍮꾧탳븯뿬 넻利 뻾쐞 諛섏쓳씠 媛먯냼맖쓣 굹깉떎. 삉븳 씠뱾怨 鍮꾧탳븯뿬 샎빀 닾뿬援곗뿉꽌뒗 164.17 ± 26.32쉶濡 굹굹 議곌뎔 諛 슱湲, 媛뺥솴, 깮媛 12.5 mg/mL 鍮꾧탳 떆 샎빀 닾뿬援곗뿉꽌 넻利 뻾쐞 諛섏쓳씠 쑀쓽븯寃 媛먯냼맖쓣 솗씤븯떎(P<0.05).

Fig. 7.

Effects of mixture on nociceptive behavior at facial area. Following administration of mixture, the nociceptive responses were reduced in 2nd phase in a dose-dependent manner. There were 6 animals in each group. *,+,#P<0.05, C.L, C.A, Z.O vs mixture 12.5 mg/mL + F.

*C.L: Curcuma longa L., +C.A: Curcuma aroma tica Salisb., #Z.O: Zingiber officinale Rosc., Mixture: combine of C.L, C.A, Z.O



떆媛꾩쓽 寃쎄낵뿉 뵲瑜 넻利 뻾쐞 諛섏쓳쓽 蹂솕뒗 떎쓬怨 媛숇떎(Fig. 8). 룷瑜대쭚由곗쓽 二쇱엯쑝濡 쑀룄맂 넻利 뻾쐞 諛섏쓳 15遺 씠썑遺꽣 利앷븯뿬 25遺꾩뿉 媛옣 넂寃 굹궗쑝硫 40遺꾧퉴吏 넻利앹씠 吏냽릺떎媛 45遺꾩뿉 媛먯냼븯떎. 12.5 mg/mL쓽 슱湲, 媛뺥솴, 깮媛뺤쓽 寃쎄뎄 닾뿬뒗 룷瑜대쭚由 二쇱엯 썑 20~35遺, 샎빀 닾뿬援곗뿉꽌뒗 20~30遺꾩뿉 룷瑜대쭚由곗쑝濡 쑀룄맂 넻利 뻾쐞 諛섏쓳쓣 쑀쓽븯寃 媛먯냼떆耳곕떎.

Fig. 8.

Changes in nociceptive responses at facial area following administration of mixture. Oral administration of mixture, significantly reduced the nociceptive responses 20~30 mim after induction of pain. There were 6 animals in each group. #P<0.05, Z.O vs mixture 12.5 mg/mL + F.

C.L: Curcuma longa L., C.A: Curcuma aroma tica Salisb., Z.O: Zingiber officinale Rosc., Mixture: combine of C.L, C.A, Z.O



븞硫대 뿼利앹꽦 넻利 뻾쐞 諛섏쓳 議곗젅뿉 슱湲, 媛뺥솴, 깮媛 異붿텧臾 諛섎났 닾뿬쓽 슚怨

떎뿕룞臾쇱쓽 삤瑜몄そ 닔뿼遺 뵾븯濡 5% 룷瑜대쭚由(50 μL) 쓣 닾뿬븯뿬 쑀룄븳 븞硫대 뿼利앹꽦 넻利앹뿉꽌 슱湲, 媛뺥솴, 깮媛 諛섎났 닾뿬쓽 넻利 뻾쐞 諛섏쓳 議곗젅 슚怨쇰뒗 떎쓬怨 媛숈씠 굹궗떎(Fig. 9). 븞硫대 遺쐞쓽 1李 넻利 뻾쐞 諛섏쓳쓽 룷瑜대쭚由 援곌낵 슜留(CMC-Na) 援곗뿉꽌 媛 29.00 ± 16.20, 15.67 ± 7.08쉶濡 굹궗怨, 슱湲, 媛뺥솴, 깮媛 12.5 mg/mL 諛섎났 닾뿬援곗뿉꽌쓽 넻利 뻾쐞 1李 諛섏쓳쓽 寃곌낵뿉꽌뒗 媛 13.33 ± 7.53쉶, 11.33 ± 6.17쉶, 7.83 ± 4.74쉶濡 굹굹 議곌뎔怨 떎뿕援 媛꾩쓽 겙 李⑥씠媛 뾾뿀떎. 넻利 뻾쐞 2李 諛섏쓳쓽 寃곌낵뿉꽌뒗 룷瑜대쭚由곌뎔怨 슜留ㅺ뎔 媛 328.41 ± 12.86, 349.33 ± 46.15쉶濡 넻利 뻾쐞 諛섏쓳씠 넂寃 굹궗쑝硫, 12.5 mg/mL쓽 諛섎났 닾뿬援곗뿉꽌 슱湲, 媛뺥솴, 깮媛뺤 媛 166.50 ± 24.48쉶, 247.33 ± 14.98쉶, 172.50 ± 57.25쉶濡 議곌뎔怨 鍮꾧탳븯뿬 넻利 뻾쐞 諛섏쓳씠 媛먯냼맖쓣 굹궗떎. 諛섎났 닾뿬援곗쓣 鍮꾧탳븯쓣 븣 媛옣 넻利 뻾쐞 諛섏쓳씠 궙寃 굹궃 닚꽌濡쒕뒗 슱湲, 깮媛, 媛뺥솴씠쑝硫 떒쉶 닾뿬 떆 蹂대떎 諛섎났 닾뿬 떆뿉꽌 넻利 뻾쐞 諛섏쓳씠 쑀쓽븯寃 媛먯냼맖쓣 솗씤븯떎(P<0.05).

Fig. 9.

Effects of repeated on nociceptive behavior at facial area. Following administration of repeated, the nociceptive responses were reduced in 2nd phase in a dose-dependent manner. There were 6 animals in each group. *P<0.05, Formalin vs C.L, C.A, Z.O 12.5 mg/mL + F.

C.L: Curcuma longa L., C.A: Curcuma aroma tica Salisb., Z.O: Zingiber officinale Rosc.



떆媛꾩쓽 寃쎄낵뿉 뵲瑜 넻利 뻾쐞 諛섏쓳쓽 蹂솕뒗 떎쓬怨 媛숇떎(Fig. 10). 룷瑜대쭚由곗쓽 二쇱엯쑝濡 쑀룄맂 넻利 뻾쐞 諛섏쓳 15遺 씠썑遺꽣 利앷븯뿬 25遺꾩뿉 媛옣 넂寃 굹궗쑝硫 40遺꾧퉴吏 吏냽릺떎媛 45遺꾩뿉 媛먯냼븯떎. 12.5 mg/mL쓽 깮媛뺢낵 떇臾쇰뱾 諛섎났 닾뿬뒗 룷瑜대쭚由 二쇱엯 썑 넻利 뻾쐞 諛섏쓳씠 利앷븯뒗 20遺 遺꽣 넂븯떎媛 30遺 씠썑濡 媛먯냼맖쓣 굹궗떎.

Fig. 10.

Effects of repeated on nociceptive behavior at facial area. Following administration of repeated, the nociceptive responses were reduced in 2nd phase in a dose-dependent manner. There were 6 animals in each group. *P<0.05, Formalin vs C.L, C.A, Z.O 12.5 mg/mL + F.

C.L: Curcuma longa L., C.A: Curcuma aroma tica Salisb., Z.O: Zingiber officinale Rosc.


怨 李

移섍낵 吏덊솚 以 븯굹씤 援ш컯븞硫 넻利앹 궣쓽 吏덇낵 諛젒븯寃 뿰愿릺뼱 엳쑝硫, 뿬윭 諛⑸㈃쑝濡 솚옄뿉寃 쁺뼢쓣 誘몄튇떎. 븞硫대 넻利앹씠 씪긽깮솢씠굹 슫룞 솢룞 諛 궗쉶쟻 솢룞뿉 誘몄튂뒗 쁺뼢 씠誘 꽑뻾 뿰援ъ뿉꽌룄 諛앺議뚯쑝硫(Maulina et al., 2018), 씠泥섎읆 븞硫대 넻利앹 궣쓽 吏덇낵 諛젒븳 뿰愿꽦씠 엳쑝誘濡 넻利앹쓽 諛쒖깮 삁諛⑷낵 移섎즺媛 븘닔쟻쑝濡 슂援щ맂떎. 洹몃윭굹 븞硫대 뿼利앹꽦 넻利 議곗젅뿉 븳 뿰援щ뒗 쁽옱 遺議깊븯떎怨 궗猷뚮릺뼱, 蹂 뿰援ъ뿉꽌뒗 떎뿕룞臾쇱쓽 븞硫대뿉 룷瑜대쭚由곗쓣 二쇱엯븳 뿼利앹꽦 넻利 紐⑤뜽쓣 쟻슜븯뿬 깮媛뺢낵 떇臾쇰뱾뿉 냽븯뒗 슱湲, 媛뺥솴, 깮媛 異붿텧臾쇱쓽 넻利 議곗젅 슚怨쇰 솗씤븯怨좎옄 븯떎.

넻利앹 깮泥댁쓽 궛솕 諛 끂솕뿉 二쇱슂 썝씤씤 궛솕 뒪듃젅뒪뿉 쓽빐꽌룄 넻利앹씠 쑀諛쒕맆 닔 엳쑝硫, 泥쒖뿰臾 삉뒗 떇뭹 벑뿉 븿쑀릺뼱 엳뒗 떎뼇븳 빆궛솕 臾쇱쭏쓽 옉슜뿉 쓽빐 議곗젅맆 닔 엳떎. 쐞븫쓽 뼲젣, 빆궛솕 슚怨, 빆洹 솢꽦 벑쓽 슚뒫씠 諛앺吏 씛誘쇰뱾젅쓽 꽑뻾 뿰援щ 蹂대㈃, RAW 264.7 꽭룷瑜 LPS濡 옄洹뱁븯쓣 븣 씛誘쇰뱾젅 臾 異붿텧臾쇱쓽 빆뿼利 슚怨쇰 議곗궗븳 寃곌낵, NO 諛 IL-1β, IL-6쓽 깮궛쓣 뼲젣븯쑝硫, MAPKs 以 p38, JNK, ERK1/2쓽 씤궛솕 Iκ-Bα쓽 遺꾪빐瑜 뼲젣븿쓣 솗씤븯뿬 씛誘쇰뱾젅媛 빆뿼利 移섎즺뿉 쓳슜맆 닔 엳떎怨 蹂닿퀬븯떎(Kim et al., 2014). 삉븳 빆븫 諛 吏꾪넻 슚怨쇨 엳뒗 媛먯넚뼢(Valerianaceae)쓽 빆궛솕 諛 빆뿼利 슚怨쇰 븣븘蹂 떎뿕뿉꽌뒗 媛먯넚뼢 85% 硫뷀깂삱 異붿텧臾쇱 in vitro뿉꽌 媛뺥븳 빆궛솕 솢꽦씠 솗씤릺뿀쑝硫, LPS濡 옄洹밸맂 伊먯쓽 蹂듦컯 macrophage뿉꽌 뿼利앹꽦 留ㅺ컻 臾쇱쭏씤 NO쓽 깮꽦씠굹 iNOS, COX-2 벑쓽 諛쒗쁽쓣 뼲젣븯뿬 媛먯넚뼢씠 룞留κ꼍솕굹 愿젅뿼 벑쓽 궛솕쟻 뒪듃젅뒪 愿젴씠 엳뒗 留뚯꽦 뿼利앹꽦 吏덊솚쓽 移섎즺뿉 슚怨쇱쟻쑝濡 궗슜맆 닔 엳쓣 寃껋쑝濡 蹂닿퀬븯떎(Baek et al., 2009). 씠윭븳 뿰援 寃곌낵 留덉갔媛吏濡 蹂 뿰援ъ뿉꽌 슱湲, 媛뺥솴, 깮媛 異붿텧臾쇱쓽 寃쎄뎄 닾뿬뒗 룷瑜대쭚由곗쑝濡 쑀룄븳 떎뿕 紐⑤뜽쓽 븞硫대 뿼利앹꽦 넻利 뻾쐞 諛섏쓳쓣 냽룄 쓽議댁쟻쑝濡 媛먯냼맖쓣 솗씤븯떎. 씠 媛숈 寃곌낵뒗 깮媛뺢낵 떇臾쇰뱾쓽 깮由 솢꽦 꽦遺꾩씠 뿼利앹꽦 넻利앹쓽 議곗젅뿉 愿뿬븳떎뒗 寃껋쓣 굹궡硫, 븞硫대 넻利 議곗젅뿉 슚怨쇱쟻쑝濡 쟻슜븷 닔 엳쓣 寃껋쑝濡 깮媛곷맂떎.

援ш컯怨 愿젴맂 넻利앹씤 떊寃쎈퀝 蹂꽦 넻利앹 留먯큹떊寃쎌쓽 넀긽쑝濡 씤빐 諛쒖깮븳떎. 뿼利(inflammation)씠 macrophage굹 mast cell 벑쓽 諛깊삁援ъ뿉꽌 깮꽦맂 떎뼇븳 떊샇쟾떖臾쇱쭏씠 愿뿬븯뒗 씪젴쓽 蹂묐━쟻씤 怨쇱젙쑝濡쒖꽌, 씠 以 macrophage뒗 몴쟻씤 뿼利 留ㅺ컻臾쇱씤 NO (nitric oxide) 깮꽦뿉 愿뿬븳떎. NO뒗 湲됱꽦 諛 留뚯꽦 뿼利앸컲쓳뿉꽌 L-arginine쑝濡쒕꽣 iNOS (inducible nitric oxide synthase)뿉 쓽빐 怨쇰웾 깮꽦릺硫 뿼利앸컲쓳쓣 珥됱쭊떆궎뒗 寃껋쑝濡 븣젮졇 엳떎. iNOS뒗 LPS 옄洹뱀뿉 쓽빐 諛쒗쁽릺뒗뜲 씠뒗 怨쇰웾쓽 NO瑜 깮꽦븯寃 릺뼱 꽭룷뿉 궛솕 뒪듃젅뒪瑜 二쇨쾶 릺硫(Baek et al., 2009; Kim et al., 2014), 씠瑜 젣嫄고븯뒗 깮泥 궡쓽 諛⑹뼱 떆뒪뀥怨쇱쓽 洹좏삎씠 臾대꼫吏硫 븫쓣 鍮꾨’븳 뿬윭 吏덈퀝쓽 諛쒕퀝뿉 愿뿬븯誘濡 궛솕 뒪듃젅뒪瑜 젣嫄고븯뒗 빆궛솕 臾쇱쭏 吏吏 怨쇱궛솕 옄쑀씪뵒移쇱쓣 빐븯뒗 벑쓽 怨쇱젙쓣 넻빐 뿬윭 吏덊솚쓽 諛쒕퀝쓣 셿솕븷 닔 엳뒗 썑蹂 臾쇱쭏씠 맆 닔 엳떎怨 븯떎(Lee et al., 2013). 깮媛뺢낵 떇臾 以 슱湲, 媛뺥솴쓽 꽦遺꾩뿉 룷븿맂 curcumin 꽑뻾 뿰援ъ뿉꽌 씠誘 빆궛솕 臾쇱쭏濡쒖꽌쓽 슚슜씠 諛앺졇 엳떎. Curcumin 깮媛뺢낵 떇臾쇱씤 슱湲덉씠굹 媛뺥솴뿉꽌 異붿텧븳 꽦遺꾩쑝濡 빆궛솕 臾쇱쭏, 빆븫젣, 빆諛붿씠윭뒪젣濡 留롮씠 씠슜맂떎. 삉븳 궛솕뿉 쓽븳 DNA 넀긽쓣 諛⑹븯怨, free radical쓣 냼嫄고븯뒗 뒫젰씠 쎇뼱굹떎怨 븣젮졇 엳떎(Jeon and Kim, 2013). 씛 伊먯뿉 怨좎諛, 怨좎퐳젅뒪뀒濡 떇씠 븿猿 슱湲 遺꾨쭚쓣 냽룄 蹂꾨줈 怨듦툒븯뿬 빆궛솕怨 諛 궛솕쟻 넀긽뿉 誘몄튂뒗 쁺뼢쓣 愿李고븳 뿰援ъ뿉꽌 슱湲덉쓣 닾뿬븳 mice뿉꽌 媛꾩“吏곸쓽 꽭룷留됱뿉 빆궛솕젰씠 利앷븯怨 엳떎怨 蹂닿퀬븯쑝硫, 슱湲덉 議곗쭅 以묒쓽 怨쇱궛솕吏吏덉쓽 븿웾쓣 媛먯냼떆궎뒗 슚怨쇨 엳뒗 寃껋쑝濡 굹굹 슱湲덉뿉 븿쑀맂 쑀湲곗궛 벑 뿬윭 빆궛솕怨 臾쇱쭏씠 議곗쭅쓽 궛솕瑜 뼲젣븯뒗 빆궛솕怨 湲곕뒫쓣 솗씤븯떎(Kim et al., 2013). 삉븳 湲됱꽦 諛 留뚯꽦 뿼利 紐⑤뜽뿉꽌쓽 媛뺥솴 異붿텧臾쇱쓽 빆뿼利 슚怨쇰 뿰援ы븳 떎뿕뿉꽌뒗 湲됱꽦 뿼利앹뿉 븳 슚怨쇰 븣븘蹂닿린 쐞븯뿬 carrageenan뿉 쓽븳 議깅醫 쑀諛쒓낵 xylene뿉 쓽븳 洹遺醫 쑀諛쒖쓣 씪쑝궓 紐⑤뜽뿉 媛뺥솴 異붿텧臾쇱쓣 냽룄 蹂꾨줈 寃쎄뎄 닾뿬븯쓣 븣 뿼利 뼲젣쑉씠 넂 寃껋쑝濡 굹궗쑝硫, 쑁븘醫 떎뿕뿉꽌뒗 議곌뎔怨 鍮꾧탳븯뿬 궪異쒕Ъ怨 뿼利 쑁븘醫낆씠 쟻寃 諛쒖깮맂 寃껋쑝濡 굹궗떎. 뵲씪꽌 媛뺥솴쓣 寃쎄뎄 닾뿬븯쓣 븣 留뚯꽦 諛 湲됱꽦 뿼利앹뿉 븳 빐 솢꽦씠 엳뒗 寃껋쑝濡 굹굹 빆뿼利 냼옱濡쒖꽌 媛쒕컻 媛뒫꽦씠 엳쓬쓣 젣떆븯떎(Anandakumar et al., 2014). 씠윭븳 꽑뻾 뿰援щ뱾濡 鍮꾩텛뼱蹂 븣 깮媛뺢낵 떇臾쇱 떎뼇븳 떎뿕 紐⑤뜽뿉꽌 빆궛솕 옉슜 諛 빆뿼利 옉슜쓣 솗씤븯떎. 洹몃윭굹 깮媛뺢낵 떇臾 異붿텧臾쇱씠 援ш컯븞硫대뿉 誘몄튂뒗 쁺뼢怨 愿젴맂 뿰援щ뒗 븘吏곴퉴吏 誘몃명븯떎. 蹂 뿰援ъ뿉꽌뒗 븞硫대 넻利 議곗젅 뒫젰쓣 룊媛븯湲 쐞븯뿬 떎뼇븳 뿰援ъ뿉꽌 빆궛솕, 빆뿼利 슚怨쇨 엯利앸맂 깮媛뺢낵 떇臾쇰뱾쓣 떎뿕 紐⑤뜽뿉 쟻슜븯쑝硫, 룷瑜대쭚由곗쑝濡 쑀룄븯뿬 利앷븳 넻利 뻾쐞 諛섏쓳 寃쎄뎄濡 二쇱엯맂 슱湲, 媛뺥솴, 깮媛뺤뿉 쓽빐 냽룄 쓽議댁쟻쑝濡 媛먯냼릺뿀떎. 씠윭븳 寃곌낵뒗 룷瑜대쭚由곗쑝濡 쑀諛쒕맂 븞硫대 뿼利앹꽦 넻利 諛쒖깮뿉 깮媛뺢낵 떇臾쇰뱾씠 議곗젅뿉 愿뿬븿쓣 븣 닔 엳떎. 삉븳 媛곴컖쓽 빟臾쇱쓣 떒쉶 닾뿬뻽쓣 떆 蹂대떎 슱湲, 媛뺥솴, 깮媛 異붿텧臾쇱쓽 냽룄瑜 샎빀븳 샎빀 닾뿬援곗뿉꽌뒗 뜑 넂 넻利 議곗젅 슚怨쇰 솗씤븯쑝硫, 씠윭븳 寃곌낵 鍮꾧탳븯뿬 깮媛뺢낵 떇臾쇰뱾 異붿텧臾쇱쓽 냽룄瑜 媛곴컖 諛섎났 닾뿬븳 援곗뿉꽌뒗 떒쉶 닾뿬 諛 샎빀 닾뿬뻽쓣 떆蹂대떎 諛섎났 닾뿬援곗뿉꽌 넻利앹씠 뜑 媛먯냼릺뒗 슚怨쇰 솗씤븯떎. 씠윭븳 寃곌낵濡 슱湲, 媛뺥솴, 깮媛뺤쓽 넻利 議곗젅 슚怨쇰뒗 븞硫대 뿼利앹꽦 넻利 議곗젅뿉 븘슂븳 泥쒖뿰 移섎즺젣 媛쒕컻 썑蹂 臾쇱쭏濡 솢슜븷 닔 엳쓣 寃껋쑝濡 떆궗븳떎.

ACKNOWLEDGMENT

None.

CONFLICT OF INTEREST

The authors have no conflict of interest regarding the publication of this article.

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  3. Baek S, Choi JH, Ko SH. 2009. Antioxidant and Anti-inflammatory Effect of Nardostachys Chinensis in IFN-γ/LPS-stimulated Peritoneal Macrophage. Korean J Oriental Physiology &Pathology. 2009. 23: 853-859.
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  7. Jeon YJ, Kim DY. 2013. The Antioxidant Effect of Curcumin on Miniature Pig Sperm Cryopreservation. Journal of Chitin and Chitosan. 2013. 18: 190-197.
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  17. Lee YJ, Kim EO, Choi SW. 2011. Isolation and Identification of Antioxidant Polyphenolic Compounds in Mulberry (Morus alba L) Seeds. Journal of the Korean Society of Food Science and Nutrition. 2011. 40: 517-524.
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  21. Song YJ, Ha CW. 2009. Review Articles :The Use of COX-2 Selective Nonsteroidal Anti-inflammatory Drugs for the Treatment of Osteoarthritis. Knee Surgery &Related Research. 2009. 21: 93-101.
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