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Genetic Diversity of Metallo-β-lactamase Genes of Chryseobacterium indologenes Isolates from Korea
Biomed Sci Letters 2019;25:275-281
Published online September 30, 2019;  https://doi.org/10.15616/BSL.2019.25.3.275
© 2019 The Korean Society For Biomedical Laboratory Sciences.

Jong Hwa Yum†,*

Department of Clinical Laboratory Science, Dongeui University, Busan 47340, Korea
Correspondence to: Jong Hwa Yum. Department of Clinical Laboratory Science, Dongeui University, Busan 47340, Korea.
Tel: +82-51-890-2682, Fax: +82-505-182-6877, e-mail: auxotype@deu.ac.kr
*Professor.
Received August 15, 2019; Revised September 2, 2019; Accepted September 9, 2019.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
 Abstract
This study was performed to characterize the chromosomal metallo-β-lactamases (MBLs) of Chryseobacterium indologenes isolated from Korea and to propose a clustering method of IND MBLs based on their amino acid similarities. Chromosomal MBL genes were amplified by PCR from 31 clinical isolates of E. indologenes. Nucleotide sequencing was performed by the dideoxy chain termination method using these PCR products. Antimicrobial susceptibilities were determined by the agar dilution method. PCR experiments showed that all 31 E. indologenes isolates contained all blaIND genes. DNA sequence analysis revealed that E. indologenes isolates possessed ten types of blaIND gene, including seven novel variants (blaIND-8 to blaIND-14). The most common combination of MBL was IND-2 (n = 18). Minimum inhibitory concentrations of imipenem and meropenem for the isolates harboring novel IND MBLs were ≥ 16 µg/mL. IND MBLs were grouped in three clusters, based on amino acid similarities.
Keywords : Chryseobacterium indologenes, Metallo-β-lactamase, IND, blaIND, Cluster