Search for


TEXT SIZE

search for



CrossRef (0)
Effects of Saururus chinensis BAILL Extract in Rats with Experimentally Chronic Constipation: An application of Clinical Pathology and Digital Image Processing
Biomed Sci Letters 2019;25:339-347
Published online December 31, 2019;  https://doi.org/10.15616/BSL.2019.25.4.339
© 2019 The Korean Society For Biomedical Laboratory Sciences.

Min-Seok Cho1,*, Seokyoun Choi2,***, Gilhyun Lee3,***, Mi Ha Ju4,** and Seok-Cheol Choi1,†,***

1Department of Clinical Laboratory Science, Catholic University of Pusan, Busan 46252 Korea
2Department of Radiological Science, Catholic University of Pusan, Busan 46252 Korea
3Department of Clinical Laboratory Science, Dong Eui University, Busan 47340 Korea
4Department of Pathology, Dong-A University College of Medicine, Busan 49201 Korea
Correspondence to: Seok-Cheol Choi. Department of Clinical Laboratory Science, Catholic University of Pusan, 57 Olyundaero, Geumjeong-gu, Busan 46252, Korea.
Tel: +82-51-510-0569, Fax: +82-51-510-0569, e-mail: scchoi@cup.ac.kr
*Graduate student, **Researcher, ***Professor.
Received October 15, 2019; Revised December 9, 2019; Accepted December 11, 2019.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
 Abstract

Saururus chinensis (SC) belongs to the dicotyledonous plants, and its roots, leaves and flowers are white, so it is named three hundred and seconds. It is mainly distributed in Korea, China and Japan. In Korea, it is a native plant distributed in Jejudo and Jirisan areas. It has been known to improve blood circulation, anticarcinogenic effects and purge. However, studies of the efficacy on digestive system is few. This study was conducted to evaluate the effects of oral administered-SC extract in loperimide-induced constipation rats. The amount, weight and water content of the stools were measured. The number and type of stools in the large intestines were measured, and the amount of intestinal mucus was analyzed by serological analysis and histologic special staining. The severity of constipation in SC groups was significantly less than that in control group (non-SC rats). Digital Image processing also showed weaker inflammation on the large intestines of SC groups than that of control group (non-SC group). Especially, with increased dose dependent manner of SC extract, the amount and integrity of intestinal mucus increased. These results suggest that SC extract may prevent the symptoms of constipation.

Keywords : Saururus chinensis (SC), Loperamide, Rat, Constipation, Digital image processing
꽌 濡

理쒓렐 깮솢닔以쓽 뼢怨 꽌援ы솕맂 떇뒿愿 샊 臾대━븳 떎씠뼱듃뿉 쓽빐꽌 꽦蹂 뿰졊쓣 遺덈Ц븯怨 留롮 궗엺뱾씠 蹂鍮꾩뿉 쓽긽븳 諛곕옣븷濡 씤빐 궣쓽 吏덉씠 뼥뼱吏怨 엳떎(You et al., 2010). 蹂鍮 샊 諛곕옣븷뒗 쓽븰쟻쑝濡 諛곕 슏닔媛 쟻嫄곕굹 諛곕 솢룞씠 옒뱺 긽깭瑜 留먰븳떎. 蹂鍮꾩쓽 쓽븰쟻 吏꾨떒湲곗 諛곕 슏닔媛 二 2쉶 씠븯, 蹂쓽 뼇씠 35 g 誘몃쭔, 뵳뵳븳 蹂, 遺덉셿쟾븳 諛곕 뒓굦, 諛곕 떆 湲댁옣媛먭낵 빆臾 留됲옒 利앹꽭 벑쑝濡 젙쓽븳떎(Park et al., 2013). 蹂鍮꾨뒗 늻援щ굹 쓷엳 寃る뒗 쐞옣愿 利앹꽭 以 븯굹濡 諛쒖깮 썝씤 닠븳 슂씤 쇅뿉룄 留ㅼ슦 떎뼇븯뿬 洹 빐寃곗쓣 쐞빐 媛湲됱쟻 뒿愿쟻 빟臾쇱궗슜쓽 옄젣 븿猿 洹쒖튃쟻 떇궗뒿愿, 쟻젅븳 떇뭹꽠痍 諛 깮솢뼇떇쓽 蹂솕瑜 넻빐 젙긽쟻씤 諛곕쓣 쑀룄븿쓣 엫긽쑝濡 沅뚯쑀븯怨 엳떎(Lee et al., 2009). 蹂鍮꾩쓽 쑀蹂묐쪧 16.5%씠硫 移섎즺瑜 쐞빐 誘쇨컙슂踰뺤쓣 궗슜븯뒗 궗엺 蹂鍮꾪솚옄쓽 81.5%, 빟援뿉꽌 빟쓣 蹂듭슜븳 寃쏀뿕씠 엳뒗 궗엺 91.4%濡 蹂닿퀬맂 諛 엳떎(Kim et al., 2013).

쁽옱 궗슜릺怨 엳뒗 蹂鍮 移섎즺 삉뒗 媛쒖꽑 빟臾쇱 룓뇙삎蹂鍮꾩쓽 寃쎌슦 60~80%씪뒗 넂 移섎즺슚怨쇰 굹궡怨 엳吏留 꽌뻾꽦蹂鍮 샊 옣臾대젰利앹뿉 쓽븳 蹂鍮꾩쓽 移섎즺쑉씠 議고븯뿬 슚怨쇨 寃利앸릺吏 븡 誘쇨컙슂踰뺤쑝濡 蹂鍮꾨 빐寃고븯젮뒗 궗엺뱾씠 利앷븯怨 엳뒗 異붿꽭씠떎(Cheon et al., 2007).

諛곕옣븷瑜 寃る뒗 솚옄뱾뿉 엳뼱꽌 떇씠슂踰뺤씠굹 슫룞 벑쓣 뻽쓬뿉룄 媛쒖꽑슚怨쇰 뼸吏 紐삵븷 寃쎌슦 2李⑥쟻쑝濡 빟臾 移섎즺瑜 吏꾪뻾븯뒗뜲 궪닾븬 셿솕젣 遺뵾삎꽦 셿솕젣쓽 寃쎌슦 蹂듬 뙺李쎌쓣 씪쑝궎怨 꽕궗 벑쓽 臾몄젣瑜 빞湲고븯怨 옄洹뱀꽦 븯젣瑜 궗슜븷 寃쎌슦뿉뒗 쐞옣愿怨꾪넻쓽 빀蹂묒쬆쓣 쑀諛쒗븳떎怨 븣젮졇 엳떎. 씠윭븳 湲곗〈 빟臾쇰뱾쓽 遺옉슜씠굹 臾몄젣젏쓣 怨좊젮빐蹂댁븯쓣 븣 蹂鍮 利앹꽭 媛쒖꽑씠굹 移섎즺뿉 슚怨쇨 엳뒗 깉濡쒖슫 泥쒖뿰臾쇱쭏쓽 뿰援ш 븘슂븯떎怨 뙋떒맂떎.

蹂 뿰援ъ뿉 궗슜맂 궪諛깆큹뒗 quercitrin, tannin 벑씠 留롮씠 븿쑀릺뼱 엳怨 듅엳 옂뿉뒗 Fe 꽦遺꾩씠 媛옣 留롮씠 엳떎怨 븣젮졇 엳쑝硫(Lee et al., 2000; Lee et al., 2006), 쟾넻쟻쑝濡 슂룄뿼, 쟾由쎌꽑뿼, 諛⑷킅뿼, 끂룞뵾濡, 洹쇱쑁넻, 怨⑥닔뿼뿉 슚怨쇨 엳떎怨 엳떎怨 븣젮졇 엳떎(Lee et al., 2002). 삉븳 궪諛깆큹 異붿텧臾쇱 MMP-2 MMP-9쓽 솢꽦쓣 뼲젣븯뿬 빆뿼利앺슚怨 諛 빆궛솕슚怨쇨 엳떎뒗 뿰援ш껐怨쇨 蹂닿퀬릺怨 엳떎(Kim and Kim, 2016). 蹂 떎뿕 씠윭븳 궪諛깆큹쓽 슚뒫쓣 湲곕컲쑝濡 븯뿬 湲곗큹遺꾩꽍쓽븰씤 엫긽蹂묐━븰쟻 諛⑸쾿怨 諛⑹궗꽑븰 遺꾩빞뿉꽌 쓳슜릺怨 엳뒗 뵒吏꽭쁺긽 泥섎━湲곕쾿(Digital Image processing)쓣 븿猿 쟻슜븯뿬 삉 떎瑜 愿젏뿉꽌쓽 븰臾몄쟻 쑖蹂듯빀쓽 媛뒫 뿬遺룄 븿猿 議곗궗빐 蹂댁븯떎.

옱猷 諛 諛⑸쾿

떎뿕룞臾

泥댁쨷씠 180~210 g씤 6二쇰졊 Sprague-Dawle怨(SD) 븫而 씛伊먮 遺꾩뼇 諛쏆 썑(Samtokobio Co., Korea) 7씪媛 궗쑁떎쓽 쟻쓳湲곌컙쓣 嫄곗튇 썑 臾댁옉쐞濡 6留덈━뵫 4媛쒓뎔쑝濡 遺꾨쪟븯떎. 궗쑁떎 솚寃쎌 삩룄 22±2℃, 뒿룄 55~60%瑜 쑀吏븯怨 솚뭾湲곕줈 빆긽 솚湲곗떆耳곕떎. 궗쑁떎쓽 議곕챸 12떆媛꾩쓣 씠癒몃줈 꽕젙븯뿬 궙/諛(12떆媛/12떆媛)쓣 쑀吏븯떎. Rat 쟾슜 궗猷뚯 臾쇱 留ㅼ씪 異⑸텇븳 뼇쓣 젣怨듯븯떎. 씠 뿰援щ뒗 遺궛媛넧由븰援 떎뿕룞臾쇱쑄由ш퇋젙뿉 뵲씪 吏꾪뻾븯떎(떖쓽踰덊샇: CUP AEC 2019-004).

떎뿕쟻 蹂鍮 쑀룄

Loperamide瑜 씠슜븯뿬 蹂鍮 利앹긽쓣 쑀룄븯떎. Loperamide瑜 깮由ъ떇뿼닔뿉 씗꽍븯뿬 궗슜븯쑝硫 蹂鍮 쑀諛쒖쓣 쐞빐 궗슜맂 Loperamide뒗 빟쟾뿉 뵲씪 蹂鍮 쑀룄 伊 媛 留덈━떦 븯猷 2쉶(am10:00, pm18:00) kg/0.05 mg 뵫 3씪 룞븞 寃쎄뎄닾뿬 븯떎. 3씪媛 븯猷 2쉶 Loperamide瑜 寃쎄뎄닾뿬 븯怨 PM19:00떆뿉 媛 耳씠吏 留덈떎 蹂쓽 뼇쓣 痢≪젙븯뿬 蹂鍮 利앹긽씠 쑀諛쒕릺뿀쓬쓣 솗씤븯떎.

궪諛깆큹 異붿텧븸 젣議

궪諛깆큹(Saururus chinensisBAILL, 씠븯 SC)뒗 寃쎈턿 쁺泥쒖떆 냼옱 냽뾽쉶궗踰뺤씤 몢넀븷 빟珥덉뿉꽌 援ъ엯븯떎. 궪諛깆큹瑜 쓲瑜대뒗 臾쇱뿉 源⑤걮씠 뵽 썑 洹몃뒛뿉 씪二쇱씪 씠긽 嫄댁“떆耳 遺꾩뇙湲곕 씠슜븯뿬 옒寃 遺꾩뇙븳 썑 諛뺥뀒由ъ븘 벑쓽 삤뿼 諛⑹ 諛 臾쇱쭏쓽 넀긽쓣 理쒖냼솕븯湲 쐞븯뿬 룞寃곌굔議 떆耳곕떎. 룞寃 嫄댁“맂 떆猷 3 g怨 70% ethanol 10 mL瑜 15 mL tube뿉 꽔뼱 샎빀湲곗뿉꽌 24떆媛 룞븞 옄뿰諛섏쓳 떆궓 썑 2,500 rpm쑝濡 썝떖遺꾨━븯뿬 긽痢듭븸留 梨꾩랬븯떎. 梨꾩랬맂 긽痢듭븸쓣 42℃뿉꽌 궓 뿉깂삱 꽦遺꾩쓣 異⑸텇엳 利앸컻떆궓 썑 궓 떆猷뚮 냽異뺤떆耳 룞寃 嫄댁“븯뿬 궗슜븯떎.

떎뿕쓽 꽕怨

떎뿕슜 伊 SD 醫낆쓣 떎쓬怨 媛숈씠 媛곴컖 떎뿕룞臾쇨뎔쑝濡 遺꾨쪟븯떎.

  • 洹몃9 I: 議곌뎔쑝濡 깮由ъ떇뿼닔留뚯쓣 寃쎄뎄닾뿬븳 伊(n=10)

  • 洹몃9 II: Loperamide濡 蹂鍮꾨 쑀諛쒗븯怨 깮由ъ떇뿼닔瑜 寃쎄뎄닾뿬븳 떎뿕援(n=10)

  • 洹몃9 III: Loperamide濡 蹂鍮꾨 쑀諛쒗븯怨 궪諛깆큹 異붿텧臾(200 mg/kg)쓣 寃쎄뎄닾뿬븳 떎뿕援(n=10)

  • 洹몃9 IV: Loperamide濡 蹂鍮꾨 쑀諛쒗븯怨 궪諛깆큹 異붿텧臾(100 mg/kg)쓣 寃쎄뎄닾뿬븳 떎뿕援(n=10)

떎뿕 씪젙

떎뿕 珥 5二쇨컙 吏꾪뻾븯뒗뜲, 1二쇱씪 궗쑁떎 쟻쓳湲곌컙, 洹몃━怨 굹癒몄 4二쇨컙 떎젣 떎뿕湲곌컙 씠뿀떎. 떎뿕 蹂鍮 쑀諛 쟾(0~25씪)怨 쑀諛 썑(26~28씪)濡 굹닠 吏꾪뻾븯떎. 쑀諛 쟾怨 쑀諛 썑 룞臾쇰뱾쓽 蹂쓽 媛쒖닔 닔遺꾪븿웾쓣 寃궗怨, 28씪李⑥뿉 蹂쓽 옣 넻怨쇱떆媛꾩쓣 寃궗븯떎. 洹몃9 III怨 洹몃9 IV뒗 Loperamide濡 씤븳 蹂鍮 쑀諛 쟾 0씪李⑤꽣 궪諛깆큹 異붿텧臾쇱쓣 寃쎄뎄닾뿬 븯쑝硫, 留덉留 궇(28씪) 룞臾쇱쓣 씗깮떆耳곕떎.

룞臾쇱쓽 씗깮怨 寃泥 梨꾩랬

룞臾쇱쓽 씗깮: 씗깮 쟾궇 궗猷뚮줈 젣嫄고븯뿬 湲덉떇떆耳곗쑝硫, 臾쇱 異⑸텇엳 怨듦툒븯떎. 蹂듦컯二쇱궗瑜 넻빐 빟臾쇱쓣 二쇱엯븯뒗 留덉랬瑜 넻빐꽌 諛쒖깮븷 닔 엳뒗 옣愿怨꾩쓽 넀긽쓣 諛⑹븯湲 쐞빐 씉엯 留덉랬瑜 꽑깮븯쑝硫 뿉뀒瑜대 씠슜븳 씉엯 留덉랬瑜 븯떎. 留덉랬 썑 蹂듬젙留μ뿉꽌 5 cc 씠긽 삁븸쓣 梨꾩랬븯뿬 씗깮쓣 吏꾪뻾븯떎.

寃泥 梨꾩랬: 蹂 뿰援щ뒗 떎뿕룞臾쇱쓽 留뱀옣뿉꽌 吏곸옣源뚯쓽 옣湲곕 쟻異쒗븯뒗뜲 엳뼱꽌 삁븸씠 議곗쭅쑝濡 諛⑹텧 썑 뒪硫곕뱶뒗 寃껋쓣 諛⑹븯怨 쑁븞쟻 愿李곗쓣 쐞븯뿬 蹂듬젙留μ뿉꽌 삁븸쓣 5 cc 씠긽 梨꾩랬븯떎(Lee et al., 2009). 梨꾩랬븳 留뱀옣뿉꽌遺꽣 吏곸옣源뚯쓽 옣湲곕뒗 쟻異쒗븳 썑 10% formaldehyde濡 怨좎젙븯뿬 議곗쭅븰쟻 遺꾩꽍 쟾源뚯 깋옣蹂닿 븯떎.

遺꾩꽍빆紐 諛 遺꾩꽍諛⑸쾿

泥댁쨷, 궗猷, 궗猷 꽠痍⑤웾 痢≪젙: 泥댁쨷 궗쑁떆꽕 쟻쓳씠 걹궃 썑瑜 떆옉쑝濡 꽕젙븯怨 씗깮븯湲 吏곸쟾 泥댁쨷쓣 理쒖쥌 臾닿쾶濡 痢≪젙븯떎. 궗猷 꽠痍⑤웾 蹂鍮 쑀諛 쟾뿉뒗 3씪 媛꾧꺽쑝濡 痢≪젙븯怨 蹂鍮꾨 쑀諛쒗븳 씠썑뒗 븯猷⑥뿉 븳踰 痢≪젙븯떎.

蹂쓽 媛쒖닔, 以묐웾 諛 닔遺꾪븿웾 痢≪젙: 媛 떎뿕援곗쓽 蹂 蹂鍮 쑀諛 쟾怨 썑瑜 굹늻뼱 닔嫄고뻽쑝硫, 닔嫄곕뒗 떎뿕 떆옉遺꽣 留ㅼ씪 耳씠吏 븞쓽 蹂쓣 紐⑤몢 닔嫄고븯뿬 蹂쓽 媛쒖닔 諛 以묐웾쓣 痢≪젙븯떎. 媛쒖껜蹂 蹂쓽 닔遺꾪븿웾 蹂쓣 70℃ 嫄댁“湲곗뿉꽌 24떆媛 嫄댁“ 썑 嫄댁“ 쟾썑 以묐웾쓽 李⑤ 씠슜븯뿬 닔遺꾪븿웾쓣 닔移섑솕 븯떎.

옣 넻怨쇱떆媛 痢≪젙: 蹂鍮 쑀諛 3씪 썑 떎뿕룞臾쇱쓣 12떆媛 젅떇떆궓 뮘, 옣 넻怨 몴吏臾쇱쭏씤 carmine red (Sigma, USA) 1 g쓣 궗猷 5 g뿉 샎빀븯뿬 湲됱뿬븳 븳 썑 룞臾쇱씠 떇씠瑜 떆옉븯뒗 寃껋쓣 떆옉떆젏쑝濡 怨꾩궛븯뿬 Carmine red뿉 쓽빐꽌 遺됱깋쓣 쓣뒗 蹂씠 議곌툑씠씪룄 굹삤硫 洹 떆젏쓣 醫낅즺떆젏쑝濡 痢≪젙븯떎.

옣愿 궡 옍瑜 蹂 媛쒖닔 痢≪젙怨 삎깭 愿李: 옣愿 궡 옍瑜 蹂쓽 媛쒖닔뒗 떎뿕룞臾쇱쓣 씗깮븳 썑 留뱀옣遺꽣 吏곸옣源뚯 쟾泥 遺쐞瑜 젅떒븯뿬 10% formaldehyde 씤궛뿼셿異⑹븸(phosphate buffered saline, PBS, pH 7.4)쑝濡 怨좎젙빐꽌 옣 븞뿉 궓븘엳뒗 蹂쓽 겕湲곗 媛쒖닔瑜 쑁븞 愿李고븯떎.

議곗쭅蹂묐━븰쟻 遺꾩꽍(옣愿 궡 젏븸吏 遺꾨퉬슚怨 鍮꾧탳): 留뱀옣遺꽣 吏곸옣源뚯 옣愿 쟾泥대 쟻異 썑 10% formaldehyde 븸쑝濡 怨좎젙븯뿬 議곗쭅 쟾泥섎━ 怨쇱젙쓣 嫄곗튂怨 뙆씪쑝濡 룷留ㅽ븯떎. 뙆씪젅렪 4 μm 몢猿섎줈 젅렪쓣 젣옉븯怨, periodic acid Schiff (PAS) 듅닔뿼깋쓣 븳 썑 愿묓븰 쁽誘멸꼍쑝濡 愿李고븯떎.

뵒吏꽭쁺긽 泥섎━湲곕쾿: 遺꾩꽍 寃異 쁺긽泥섎━ 븣怨좊━利섏쓣 씠슜븯뿬 諛섏옄룞(semi-automatic) 삎떇쑝濡 怨꾩궛븯怨. 쁺긽뿉 議댁옱븯뒗 諛깊꽣 삎깭濡 議댁옱븯뒗 뵿 M媛쒖쓽 뜲씠꽣뿉 븯뿬 뜲씠꽣媛 냽븳 援곗쭛 以묒떖怨 뜲씠꽣 媛 嫄곕━ 李⑥씠媛 理쒖냼媛 릺룄濡 뜲씠꽣뱾쓣 N媛쒖쓽 援곗쭛 S = {s1, s2, s3,…, sN} 援곗쭛쓽 닔뒗 control 3媛, high 4媛, low 6媛, vehicle 6媛쒕 쟻슜븯뿬 怨꾩궛븯떎. 理쒖쟻솕 媛믪쑝濡 怨꾩궛 썑 뵿媛 50 씠긽쓽 紐⑤뱺 뵿쓽 빀쓣 怨꾩궛븯뿬 硫댁쟻쓣 怨꾩궛븯떎(Shi et al., 2010; Singh et al., 2012).

떇 1) M媛쒖쓽 뜲씠꽣 {X1,X2,X3,,Xm}

떇 2) argminr,cΣm=1MΣn=1MrnmXm-ck2

뵒吏꽭쁺긽 泥섎━湲곕쾿쓣 議곗쭅蹂묐━븰쟻 遺꾩꽍뿉 젒紐⑺븯뿬 젏븸吏덉쓽 겕湲곕 鍮꾧탳 遺꾩꽍븯떎.

옄猷뚮텇꽍 諛 넻怨꾩쿂由

紐⑤뱺 옄猷뚮뒗 룊洹 ± 몴以렪李⑤줈 몴떆븯怨, 떎뿕援곌낵 議곌뎔 궗씠쓽 怨꾨뒗 One way-ANOVA瑜 궗슜븯뿬 쑀쓽꽦쓣 利앸챸븯怨, Tukey 궗썑寃利앹쓣 븯떎. 궗슜븳 봽濡쒓렇옩 Prism씠硫 P < 0.05씪 븣 넻怨 媛믪쑝濡 쑀쓽븳 寃껋쑝濡 뙋떒븯떎.

寃 怨

泥댁쨷, 궗猷 꽠痍⑤웾 痢≪젙

떎뿕湲곌컙 룞븞 媛 援곗쓽 泥댁쨷怨 떇씠 꽠痍⑤웾 Table 1怨 媛숇떎. 蹂鍮 쑀諛 쟾(0~25씪) 룞븞 泥댁쨷利앷웾怨 떇씠 꽠痍⑤웾 洹몃9 媛꾩쓽 쑀쓽븳 李⑥씠뒗 굹굹吏 븡븯떎. 蹂鍮 쑀諛 썑(26~28씪) 룞븞 뿭떆 泥댁쨷利앷웾 쑀쓽븳 李⑥씠뒗 굹굹吏 븡븯吏留 Loperamide瑜 닾뿬븳 洹몃9뿉 엳뼱꽌 궗猷 꽠痍⑤웾씠 媛먯냼븯뒗 寃쏀뼢씠 굹궗떎. 蹂鍮 쑀諛 씠썑 洹몃9 媛꾩쓽 쑀쓽븳 李⑥씠뒗 굹굹吏 븡븯쑝誘濡 Loperamide뿉 쓽븳 蹂鍮꾧 泥댁쨷, 궗猷 꽠痍⑤웾뿉 겙 蹂솕瑜 二쇱 븡 寃껋쑝濡 궗猷뚮맂떎(Table 1).

Effect of Saururus chinensis on body weight and food intake

Group IGroup IIGroup IIIGroup IV
Body weight (g)
Start210.2±4.44205.7±4.24 205.1±5.12 204.3±6.56
Final323.7±15.1 312.9±12.48309.5±6.6311.5±9.8
Food intake (g/day)
BIC24.79±2.0325.11±1.9226.62±1.5925.54±2.50
AIC26.13±1.2220.06±1.6222.73±1.4821.36±1.24

Group I, Nomal group; Group II, Loperamide induction group; Group III, Loperamide induction + oral admission of SC extract (high dose); Group IV, Loperamide induction + oral admission of SC extract (low dose)

Abbreviation: SC, Saururus chinensis BAILL; BIC, Before induce of constipation; AIC, After induce of constipation


蹂쓽 媛쒖닔, 以묐웾

蹂쓽 媛쒖닔뒗 Fig. 2뿉꽌 솗씤븯뒗 諛붿 媛숈씠 蹂鍮꾨 쑀諛쒗븯湲 쟾 洹몃9 媛꾩쓽 李⑥씠뒗 뾾쑝굹 Loperamide瑜 닾뿬븯뿬 蹂鍮꾨 쑀諛쒗븯쓣 떆 젙긽援(Group I)쓽 蹂 媛쒖닔 66.33±7.55 Loperamide 떒룆 닾뿬 洹몃9(group II)쓽 蹂쓽 媛쒖닔 35.33±2.88瑜 솗씤븯硫 Loperamide濡 씤븳 蹂鍮 쑀諛쒖씠 옒 씠猷⑥뼱吏 寃껋쓣 븣 닔 엳떎(P<0.01).

Fig. 2. Effects of Saururus chinensis (SC) on the number of fecal pellets in four groups.

Group I, Nomal group; Group II, Loperamide induction group; Group III, Loperamide induction + oral admission of SC extract (high dose); Group IV, Loperamide induction + oral admission of SC extract (low dose). The numbers of fecal pellets in group II (at AI) (35.33±2.88) were significantly lower than those in group I (66.33±7.55) and group III (56±4.66). **P<0.01, (compared with Group I), #P<0.05, (compared with Group II). Abbreviation: BI, Before Induction constipation; AI, After Induction constipation; SC, Saururus chinensisBAILL.


蹂鍮 쑀諛 썑 Loperamide + 궪諛깆큹 異붿텧臾 怨좊냽룄 닾뿬 洹몃9(Group III) 56±4.66쓽 蹂 媛쒖닔瑜 굹깉怨, Loperamide 떒룆 닾뿬援곌낵 鍮꾧탳 떆 쑀쓽븳 蹂 媛쒖닔 利앷瑜 蹂댁떎. Loperamide + 궪諛깆큹 異붿텧臾 냽룄 닾뿬 洹몃9(Group IV)쓽 蹂 媛쒖닔뒗 49.33±4.88濡 Loperamide 떒룆 닾뿬援 鍮꾧탳 떆 蹂 媛쒖닔쓽 利앷 寃쏀뼢쓣 굹깉떎(P<0.05) (Fig. 1).

Fig. 1. Wet weight of fecal pellet in four groups.

Group I, Nomal group; Group II, Loperamide induction group; Group III, Loperamide induction + oral admission of SC extract (high dose); Group IV, Loperamide induction + oral admission of SC extract (low dose). Wet weight of fecal pellet in group II (at AI) (2.66±0.17) were significantly lower than those in group I (4.4±0.06) and group III (3.46±0.24). **P<0.01 (compare with Group I); #P<0.05 (compare with Group II). Abbreviation: BI, Before Induction constipation; AI, After Induction constipation; SC, Saururus chinensisBAILL.


蹂쓽 以묐웾 뿭떆 蹂鍮 쑀諛 쟾 쟾 洹몃9뿉꽌 쑀쓽븳 李⑥씠瑜 蹂댁씠吏 븡븯쑝굹 蹂鍮 쑀諛 썑 蹂쓽 以묐웾쓽 寃쎌슦(Fig. 3) Loperamide 떒룆 닾뿬 洹몃9(Group II) 2.66±0.17쑝濡 젙긽援(4.4±0.06)怨 鍮꾧탳븯쓣 떆 쑀쓽븳 媛먯냼瑜 굹깉怨, 궪諛깆큹 異붿텧臾 怨좊냽룄 닾뿬 洹몃9(Group III) 3.46±0.24濡 Loperamide 떒룆 닾뿬援곌낵 鍮꾧탳 떆 蹂쓽 以묐웾씠 利앷븯떎(P<0.05).

Fig. 3. Fecal water content in four groups.

Group I, Nomal group; Group II, Loperamide induction group; Group III, Loperamide induction + oral admission of SC extract (high dose); Group IV, Loperamide induction + oral admission of SC extract (low dose). Fecal water content in group II (at AI) (33.52%) were lower than those in group I (40.66), group III (38.52) and group IV (37.90%). **P<0.01 (compare with Group I); ##P<0.01 (compare with Group II). Abbreviation: BI, Before Induction constipation; AI, After Induction constipation; SC, Saururus chinensisBAILL.


蹂쓽 닔遺꾪븿웾

蹂鍮 쑀諛 쟾 蹂쓽 닔遺꾪븿웾 쟾 洹몃9뿉꽌 쑀쓽븳 李⑤ 굹궡吏 븡븯쑝硫, 蹂鍮꾨 쑀諛쒗븳 썑 젙긽援(Group I) 怨 Loperamide 떒룆 닾뿬 洹몃9(Group II) 鍮꾧탳 떆 Group I뿉꽌 40.66%, Group II뿉꽌 33.52% 蹂쓽 닔遺꾪븿웾쓣 蹂댁씠뒗 寃껋쓣 蹂댁븘 Group II뿉꽌 蹂씠 닔遺꾪븿웾 媛먯냼瑜 솗씤븯怨, Loperamide +궪諛깆큹 異붿텧臾 怨좊냽룄 닾뿬 洹몃9(Group III)뿉꽌 38.52% Loperamide + 궪諛깆큹 異붿텧臾 냽룄 닾뿬 洹몃9(Group IV)뿉꽌 37.90%瑜 솗씤븳 寃곌낵, 궪諛깆큹 異붿텧臾쇱쓣 닾뿬븳 洹몃9뱾뿉꽌 닔遺꾪븿웾쓽 利앷瑜 蹂댁씠怨 엳뒗 寃껋쓣 굹깉떎(Fig. 4) (P<0.01).

Fig. 4. Gastrointestinal transit time in four groups.

Group I, Nomal group; Group II, Loperamide induction group; Group III, Loperamide induction + oral admission of SC extract (high dose); Group IV, Loperamide induction + oral admission of SC extract (low dose). Gastrointestinal transit time in groups, group II (833.33 ±31.66 min) was longer than in groups III (711.67±55.83 min). **P<0.01 (compare with Group I); ##P<0.01 (compare with Group II). Abbreviation: SC, Saururus chinensisBAILL.


蹂쓽 옣 넻怨쇱떆媛 痢≪젙

蹂쓽 옣 넻怨쇱떆媛 寃곌낵뒗 Fig. 4濡 굹깉떎. Loperamide 떒룆 닾뿬 洹몃9(Group II)쓽 옣 넻怨쇱떆媛꾩 833.33±31.66 min쑝濡 굹궗怨, 궪諛깆큹 異붿텧臾 怨좊냽룄 닾뿬 洹몃9(Group III)뿉꽌뒗 711.67±55.83min濡 Group II 鍮꾧탳븯쓣 떆 쑀쓽븳 蹂쓽 옣 넻怨쇱떆媛 떒異뺤쓣 솗씤븯떎. 궪諛깆큹 異붿텧臾쇱쓣 냽룄 닾뿬븳 洹몃9(group IV) 750±59.16 min쑝濡 痢≪젙릺뼱 蹂쓽 옣 넻怨쇱떆媛꾩씠 떒異뺣릺뒗 寃쏀뼢쓣 솗씤븯떎(P<0.01).

옣愿 궡 옍瑜 蹂 媛쒖닔 痢≪젙怨 삎깭 愿李

떎뿕룞臾쇱쓣 씗깮븯뿬 留뱀옣遺꽣 吏곸옣源뚯 옣愿 궡 蹂 媛쒖닔 삎깭瑜 愿李고븳 寃곌낵뒗 Fig. 6 媛숇떎. Loperamide濡 蹂鍮꾨 쑀諛쒗븯吏 븡 洹몃9(Group I)뿉꽌 蹂 媛쒖닔뒗 룊洹 3±1媛쒕줈 蹂씠 媛옣 쟻寃 愿李곕릺뿀쑝硫, Loperamide濡 蹂鍮꾨 쑀룄븳 洹몃9(Group II) 蹂 媛쒖닔뒗 룊洹 7.5±1.5媛쒕줈 솗씤릺뿀떎. Loperamide濡 蹂鍮꾨 쑀諛쒗븯怨 궪諛깆큹 異붿텧臾 怨좊냽룄 닾뿬援곗뿉꽌쓽 蹂 媛쒖닔뒗 4.8±1.4媛, 궪諛깆큹 냽룄 닾뿬援곗 6.1±0.8媛쒕줈 궪諛깆큹 異붿텧臾쇱쓽 냽룄媛 넂쓣닔濡 옣愿 궡 蹂쓽 媛쒖닔媛 쟻寃 愿李곕릺뒗 寃쏀뼢쓣 蹂댁떎. 궪諛깆큹 怨좊냽룄 닾뿬援곗쓽 옣愿 궡 蹂쓣 쑁븞쑝濡 愿李고븯쓣 븣 蹂쓽 紐⑥뼇씠 鍮꾧탳쟻 씪젙븿쓣 媛吏怨 엳뒗 寃껋쓣 愿李고븷 닔 엳떎. 洹몃윭굹 Group II Group IV뿉꽌뒗 蹂쓽 겕湲곌 鍮꾧탳쟻 옉怨, 蹂쓽 紐⑥뼇씠 씪젙移 븡븯떎(Fig. 5) (P<0.05).

Fig. 5. Residual Fecal Measurement and Morphology of Intestines in four groups.

Group I, Nomal group; Group II, Loperamide induction group; Group III, Loperamide induction + oral admission of SC extract (high dose); Group IV, Loperamide induction + oral admission of SC extract (low dose). The number of feces in the large intestine of group II (7.5±1.5) was significantly higher than that of the large intestine of group I (3±1)* and group III (4.8±1.4)*. (*; P< 0.05, statistical significance compared with the Group II). Abbreviation: SC, Saururus chinensisBAILL.


Fig. 6. Effects of the Saururus chinensisBAILL on mucous secretion capacity in loperamide induced constipation rat.

Group I, Nomal group; Group II, Loperamide induction group; Group III, Loperamide induction + oral admission of SC extract (high dose); Group IV, Loperamide induction + oral admission of SC extract (low dose). Compared to Group II, mucus components were found in Groups III and Group IV more relatively. Abbreviation: SC, Saururus chinensisBAILL.


議곗쭅蹂묐━븰쟻 옣愿 궡 젏븸吏 遺꾨퉬슚怨

PAS 議곗쭅 듅닔뿼깋 썑 쁽誘멸꼍쑝濡 愿李 떆 젙긽援곗뿉 議곗쭅뿼깋긽뿉꽌뒗 留롮 젏븸꽦 臾쇱쭏씠 蹂댁씠吏留 Loperamide 떒룆 닾뿬 洹몃9(Group II) 궪諛깆큹 異붿텧臾 냽룄 닾뿬 洹몃9(Group IV)뿉꽌뒗 젏븸꽦 臾쇱쭏씠 留롮씠 蹂댁씠吏 븡怨 궪諛깆큹 異붿텧臾 怨좊냽룄 닾뿬 洹몃9(Group III)뿉꽌뒗 鍮꾧탳쟻 留롮 젏븸꽦 臾쇱쭏씠 蹂댁씠硫 젙긽 洹몃9怨 젏븸쓽 삎깭媛 씉궗븯寃 愿李곕릺뿀떎. 쁺긽泥섎━ 븣怨좊━利 湲곕쾿쓣 씠슜븯뿬 媛 洹몃9쓽 젏븸뼇쓣 鍮꾧탳븯쓣 떆 젙긽 洹몃9(Group I) 22.04%, Loperamide 떒룆 닾뿬 洹몃9 2.16%, 궪諛깆큹 異붿텧臾 怨좊냽룄 닾뿬 洹몃9 12.52%, 궪諛깆큹 냽룄 닾뿬 洹몃9 4.85%濡 궪諛깆큹 異붿텧臾쇱쓽 냽룄媛 넂쓣닔濡 옣젏留됱쓽 젏븸 깮궛 諛 쑀吏뿉 룄씠 릺뿀떎(Fig. 6).

怨 李

蹂鍮꾧 諛쒖깮븯뒗 썝씤쑝濡쒕뒗 蹂쓽 닔遺 媛먯냼濡 씤븯뿬 蹂씠 뵳뵳븯寃 援녹뼱 諛곗텧븯湲 옒뱺 긽깭瑜 留먰븯硫, 蹂 떎뿕뿉꽌뒗 吏궗젣濡 궗슜릺硫 옣愿쓽 슫룞꽦쓣 媛먯냼떆耳 옣궡 닔遺꾩씠 씉닔릺뒗 떆媛꾩쓣 吏뿰떆耳 蹂쑝濡 떎웾쓽 닔遺꾩씠 鍮좎졇굹媛뒗 꽕궗 쁽긽쓣 諛⑹븳떎怨 븣젮졇 엳뒗 Loperamide瑜 씠슜븯뿬 蹂鍮 룞臾쇰え뜽쓣 留뚮뱾뼱 떎뿕뿉 궗슜븯떎.

Loperamide뿉 쓽빐꽌 옣궡 닔遺 遺꾨퉬媛 뼲젣릺뼱 蹂鍮꾧 諛쒖깮븳 룞臾쇰え뜽쓽 寃쎌슦 蹂쓽 뼇, 닔遺꾪븿웾, 諛곕 냽룄 벑쓣 쑁븞쟻쑝濡 뙆븙븷 닔 엳뼱 뒪겕由щ떇 떎뿕씠 媛뒫븯硫, 씉궗 쁽씤뱾뿉寃 諛쒖깮븯뒗 蹂鍮꾩 쑀궗븳 利앹긽쓣 蹂댁씠硫 떎뿕쓣 吏꾪뻾븯뿬 蹂鍮 떎뿕뿉 룄씠 릺뒗 紐⑤뜽留곸씠씪 븷 닔 엳쑝硫 蹂쓽 뼇 蹂쓽 닔遺꾪븿웾 벑쓽 媛먯냼瑜 蹂댁븘 蹂鍮 利앹긽씠 諛쒖깮븳 寃껋쑝濡 媛꾩<븳떎.

븵꽌 꽌濡좎뿉꽌룄 뼵湲됲뻽벏씠 엫긽뿉꽌 蹂鍮꾨뒗 옣湲곗쟻씤 利앹긽 媛쒖꽑슚怨쇨 넂吏 븡븘 깉濡쒖슫 빟臾쇱씠 븘슂븳 긽솴뿉 吏곷㈃빐 엳떎. 쁽옱 슦由щ굹씪쓽 뿭븰쟻 듅꽦怨 엫긽 뼇긽怨 李⑥씠젏쓣 굹궡뒗 遺곷몄 쑀읇쓽 뿰援ъ옄猷뚭 遺遺꾩씠誘濡 븳誘쇨뎅뿉 留욌뒗 뿰援ш 븘슂븳 떆젏씠떎(Park et al., 2011). 쁽옱 蹂鍮꾩뿉 궗슜릺뒗 빟젣뒗 Senna, Correctol, Exlax, Senokt, Gaviscon 벑쑝濡 移섎즺뿉 궗슜릺怨 엳쑝굹, 넂 鍮꾩슜怨 遺옉슜쑝濡 궗슜씠 븳젙쟻씤 臾몄젣젏쓣 媛吏怨 엳떎. 듅엳 Tegaserod뒗 愿긽룞留μ쓣 닔異뺤떆궎嫄곕굹 뿰異뺤떆耳쒖꽌 떖媛곹븷 寃쎌슦 떖洹쇨꼍깋쓣 씪쑝궡뿉룄 遺덇뎄븯怨 蹂鍮 移섎즺뿉 궗슜릺怨 엳뒗 떎젙씠떎(Kim et al., 2015). 엫긽뿉꽌 뿭떆 蹂鍮 移섎즺쓽 湲곕낯썝移숈 移섎즺 珥덇린 媛湲됱쟻씠硫 빟臾 궗슜쓣 理쒖냼솕븯怨 깮솢뼇떇, 떇씠뒿愿쓽 蹂솕瑜 씠슜븯뿬 젙긽쟻씤 諛곕쓣 쑀룄븯뒗 寃껋쓣 썝移숈쑝濡 븯怨 엳떎(Park et al., 2011).

蹂 뿰援ъ뿉 궗슜맂 궪諛깆큹 異붿텧臾쇱쓽 븣젮吏 빟由ъ옉슜쑝濡쒕뒗 뭾룆(窯ⓩ캃), 씠눊(筌앭걀), 닔醫(麗닺뀵), 엫吏(筌든뼻), 媛꾩뿼(궄굨), 솴떖(容껆뼵), 룓졃(궨굨), 蹂룆(畑ζ캃), 怨좏삁븬(遙섋鶯) 벑쓽 移섎즺슚怨쇨 엳뒗 寃껋쑝濡 븣젮졇 엳쑝硫, 빆궛솕뒫쓣 媛吏怨 엳뼱 끂솕 諛 뿼利앹꽦 吏덊솚뿉 냼뿼슚怨쇨 엳뒗 寃껋쑝濡 蹂닿퀬릺怨 엳떎(Byun et al., 2005; Park and Lee, 2008). 삉븳 궪諛깆큹뒗 吏諛⑹꽭룷遺꾪솕뿉 以묒슂븳 뿭븷쓣 븯뒗 쟾궗씤옄씤 PPAR γ (peroxisome proliferator-activated receptor γ) C/EBPα (CCAAT/enhancer binding proteins)쓽 諛쒗쁽쓣 뼲젣떆耳 吏諛⑹쓽 異뺤쟻쓣 留됰뒗 슚怨쇰 굹궡뒗 寃껋쑝濡 蹂닿퀬릺뿀떎(Shin et al., 2012).

蹂 뿰援ъ뿉꽌뒗 Loperamide뿉 쓽빐꽌 泥댁쨷쓽 蹂솕, 떇씠 꽠痍⑤웾쓽 겙 蹂솕뒗 諛쒖깮븯吏 븡븯怨 씠뒗 湲곗〈뿉 蹂鍮 媛쒖꽑 愿젴 끉臾몄뿉꽌룄 Loperamide뿉 쓽븳 泥댁쨷, 떇씠 꽠痍⑤웾쓽 蹂솕뒗 쑀쓽븳 李⑥씠媛 뾾떎뒗 寃껉낵 씪移섎맂떎(Jang et al., 2016).

궪諛깆큹 異붿텧臾 닾뿬 洹몃9怨 Loperamide 떒룆 닾뿬 洹몃9쓣 긽쟻쑝濡 鍮꾧탳뻽쓣 븣 궪諛깆큹 異붿텧臾 닾뿬 洹몃9(Group III, Group IV)뿉꽌 蹂쓽 뼇씠 留롮븘吏怨, 蹂쓽 닔遺꾪븿웾 뿭떆 利앷븯떎. 肉먮쭔 븘땲씪 諛곕 냽룄 뿭떆 떒異뺣릺뼱 궪諛깆큹 異붿텧臾쇱씠 蹂鍮 셿솕뿉 룄씠 맂떎怨 쓽떖븷 닔 엳떎(Fig. 1, 2, 3, 4).

蹂쓽 뼇, 以묐웾怨 蹂쓽 닔遺꾪븿웾 利앷뒗 蹂鍮꾩쓽 셿솕뿉 湲띿젙쟻씤 떊샇씠怨 諛곕 솢룞씠 썝솢븯寃 씠猷⑥뼱 엳뒗 吏몴濡(Wang et al., 2009) 궪諛깆큹媛 蹂鍮 셿솕뿉 룄씠 맂떎怨 궗猷뚮맂떎.

씠뒗 궪諛깆큹 옂뿉꽌 諛쒓껄릺뒗 꽦遺꾩씤 섎Ⅴ떆듃由곗 삁愿 벑 泥대궡뿉 遺꾪룷븯怨 엳뒗 肄쒕젅뒪뀒濡ㅼ쓣 슚怨쇱쟻쑝濡 諛곗텧빐 二쇨퀬 荑좎뿉瑜댁튂듃由곌낵 뵆씪蹂대끂씠뱶 꽦遺꾩쑝濡 씤빐 옣愿怨꾩뿉꽌 빆궛솕 옉슜쓣 븯硫 諛쒖깮떆耳 蹂씠 留됲옒뾾씠 옒 굹삤룄濡 쑀룄븯뒗 寃껋쑝濡 궗猷뚮맂떎(Zuo et al., 2015). 씠윭븳 옉슜뱾뿉 쓽빐꽌 蹂쓽 옣넻怨쇱떆媛 뿭떆 Group II 鍮꾧탳뻽쓣 떆 궪諛깆큹 異붿텧臾쇱쓣 蹂듭슜븳 Group III, Group IV뿉꽌 떆媛꾩씠 떒異뺣릺뿀쑝硫 諛곕씠 鍮⑤━ 굹삤寃 븯뒗 씠쑀뒗 궪諛깆큹 옂뿉 議댁옱븯뒗 떇씠꽟쑀냼뿉 쓽븳 쁺뼢씠 겙 寃껋쑝濡 궗猷뚮맂떎.

궪諛깆큹뿉 議댁옱븯뒗 떇씠꽟쑀냼뒗 옣愿 궡 蹂쓽 뼇怨 紐⑥뼇뿉꽌룄 쁺뼢쓣 븣 닔 엳뿀떎. Fig. 1뿉 쓽븯硫 12떆媛 젅떇 썑 control 洹몃9씤 Group I 媛숈 寃쎌슦 옣愿뿉 蹂쓽 뼇씠 븳 媛 궡쇅濡 留롮 븡쑝굹 Loperamide瑜 씠슜븯뿬 蹂鍮꾨 쑀諛쒖떆궓 Group II뿉꽌뒗 옣愿 궡뿉 蹂씠 떎웾 議댁옱븯뿬 肉먮쭔 븘땲씪 紐⑥뼇씠 젣媛곴린 떎瑜닿쾶 삎꽦릺뼱 엳怨, 궪諛깆큹 異붿텧臾 怨좊냽룄瑜 蹂듭슜떆궓 Group III뿉꽌뒗 옣愿 궡 蹂씠 Group I怨 鍮꾩듂븯硫 紐⑥뼇룄 씪젙븯떎. 궪諛깆큹 異붿텧臾 냽룄瑜 蹂듭슜떆궓 Group IV瑜 蹂대㈃ 蹂씠 떎웾 愿李곕릺怨 엳쑝굹 옣愿 궡 議댁옱븯뒗 蹂쓽 紐⑥뼇씠 씪젙븯떎뒗 寃껋씠 Group II쓽 李⑥씠젏쑝濡 蹂댁씤떎. 씠 媛숈 寃곌낵뒗 떇씠꽟쑀냼 蹂鍮꾩쓽 愿怨꾩뿉 뵲瑜 諛곕옉슜쓽 꽑뻾뿰援ъ뿉꽌 븣 닔 엳벏씠 궪諛깆큹 옂뿉 룷븿릺뼱 엳뒗 떇씠꽟쑀냼濡 씤븯뿬 씪젙븳 蹂쓽 紐⑥뼇쓣 삎꽦븯뒗 寃껋뿉 엳뼱꽌 옣뿉 臾대━媛 媛吏 븡쓣 留뚰겮쓽 겕湲곕 留뚮뱾怨 옣愿怨꾩뿉 吏냽쟻씤 씪젙븳 븬젰쓣 媛븯뿬 諛곕 솢룞뿉 룄씠 릺뿀뜕 寃껋쑝濡 궗猷뚮맂떎(Lee et al., 2008).

蹂 뿰援щ뒗 궪諛깆큹媛 옣愿怨꾩쓽 젏븸꽦 臾쇱쭏 쑀吏뿉 룄쓣 以 寃껋씠씪뒗 媛꽕 븯뿉 옣愿怨꾩쓽 젏븸臾쇱쭏쓣 솗씤븷 닔 엳뒗 듅닔뿼깋 PAS 뿼깋쓣 吏꾪뻾븯떎(Fig. 6).

蹂鍮꾧 諛쒖깮븯쓣 떆 옣愿怨꾩쓽 슫룞꽦씠 媛먯냼븯寃 릺怨 씠뿉 뵲씪꽌 옣愿怨꾩쓽 젏븸吏 痢듭쓽 몢猿 諛 젏븸吏덉씠 媛먯냼븯怨, 씠濡 씤븯뿬 옣愿 븞뿉꽌 蹂쓽 씠룞뿉 臾몄젣瑜 빞湲고븳떎怨 蹂닿퀬릺뿀떎(Cepinskas et al., 1993). 蹂 뿰援ъ뿉꽌 옣 議곗쭅 PAS 듅닔뿼깋븳 썑 쁺긽泥섎━ 븣怨좊━利 湲곕쾿쓣 씠슜븯뿬 議곗쭅쓽 듅닔뿼깋맂 遺遺꾨쭔 슚怨쇱쟻쑝濡 굹궦 썑 듅닔뿼깋 遺쐞瑜 닔移섑솕 븳 寃껋쑝濡 寃곌낵쓽 떊猶곕룄瑜 넂씪 닔 엳뿀떎. 씠윭븳 湲곕쾿쓣 넻빐 젏븸臾쇱쭏쓣 愿李고븳 寃곌낵 Group II뿉 鍮꾪븯뿬 Group III뿉꽌 젏븸臾쇱쭏쓽 媛쒖꽑씠 굹궗쑝굹 Group IV뿉꽌뒗 젏븸臾쇱쭏씠 겕寃 媛쒖꽑릺吏 븡븘 궪諛깆큹 異붿텧臾 怨좊냽룄뿉꽌 젏븸臾쇱쭏쓽 媛쒖꽑씠 굹궗떎. 궪諛깆큹뿉 떎웾쑝濡 뱾뼱 엳떎怨 븣젮졇 엳뒗 깂땶 媛뺣젰븳 빆궛솕 뒫젰쓣 媛吏怨 엳쑝硫 빆洹좎옉슜 빆븣윭吏 옉슜, 怨좎삁利 뼲젣옉슜 벑쓽 슚怨쇰 궦떎怨 븣젮졇 엳쑝硫 蹂 뿰援ъ뿉꽌 옣愿怨꾩쓽 젏븸 꽦遺 媛쒖꽑쓽 寃곌낵뒗 뵆씪蹂대끂씠뱶 꽦遺꾩쓽 씪醫낆씤 깂땶(Tannin) 꽦遺꾩쑝濡 씤븳 삁븸닚솚 媛쒖꽑쑝濡 씤븳 옣愿怨꾩쓽 슫룞꽦 利앹쭊쑝濡 젏븸 꽦遺꾩씠 媛쒖꽑 諛 쑀吏릺뿀쓣 寃껋씠씪怨 삁痢≫븳떎(Hwang, 2005).

蹂 뿰援ъ쓽 寃곌낵瑜 醫낇빀빐蹂대㈃ 궪諛깆큹 異붿텧臾쇱 Loperamide쑝濡 씤븯뿬 諛쒖깮맂 蹂鍮 利앹긽뿉 엳뼱꽌 移섎즺슚怨쇰 蹂댁쑝硫, 냽룄媛 넂 궪諛깆큹 異붿텧臾쇱씠 뜑슧 슚怨쇱쟻쑝濡 蹂鍮 利앹긽 셿솕뿉 룄씠 릺뿀떎. 씠윭븳 寃곌낵뱾쓣 넻빐 궪諛깆큹 異붿텧臾쇱 깉濡쒖슫 蹂鍮 셿솕젣濡 뿭븷쓣 븷 닔 엳쓣 寃껋쑝濡 궗猷뚮맂떎.

ACKNOWLEDGEMENT

씠 뿰援щ뒗 遺궛媛넧由븰援먯쓽 븰닠뿰援ш린湲덉쓣 吏썝諛쏆븘 닔뻾맂 뿰援ъ엫.

CONFLICT OF INTEREST

No potential conflict of interest relevant to this article was reported.

References
  1. Byun HK, Shin YW, Kim EI, Kim SM, Lee JE, Yoo DY. Effect of Saururi Herba Seu Rhizoma on anti-inflammatory properties in RAW264.7 cell Line and murine models of inflammation. The Journal of Oriental Obstetric and Gynecology 2005. 18: 51-71.
  2. Cepinskas G, Specian RD, Kvietys PR. Adaptive cytoprotection in the small intestine: role of mucus. American Journal of Physiology-Gastrointestinal and Liver Physiology 1993. 264: G921-G927.
    Pubmed CrossRef
  3. Cheon JH, Yoon IJ, Myung SJ, Byeon JS, Ko JE, Jung KW, Kim BJ, Kwon SH, Do MY, Kim DH, Ye BD. Use of constipation remedies not prescribed by physician-a study of patients at a constipation clinic. Kor J Neurogastroenterol Motil 2007. 13: 45-52.
  4. Hwang EK. The Effect of Supplementary Feeding of Tannic acid on Growth and Hematological Changes in Rats Fed High Fat Diet. Journal of Veterinary Clinics 2005. 22: 108-113.
  5. Jang JH, Jung HK, Sim MO, An BK, Kim MS, Kang BM, Cho JH, Bean CG, Kim SC, Jung WS. Effect of Dendrobium speciosum Var. Aqueous Extract on Loperamide-Induced Constipation in Rats. Korean Journal of Medicinal Crop Science 2016. 24: 1-6.
    CrossRef
  6. Kim JH, Kim EJ. Effect of Anti-oxidant, Anti-inflammatory and Anti-invasive of PMA-induced Matrix Metalloproteinase (MMP-2) and MMP-9 Activities of Water Extract and SolventFractions of Saururus chinensis. Journal of Life Science 2016. 26: 584-591.
    CrossRef
  7. Kim DH, Myung SJ, Yang DH, Yoon IJ, Seo SY, Ku HS, Yoon SM, Kim KJ, Ye BD, Byeon JS, Yang SK, Kim JH. Characteristics of Korean patients with constipation who visited a tertiary referral center. The Korean Journal of Medicine 2013. 84: 49-61.
    CrossRef
  8. Kim JE, Park SH, Kwak MH, Go J, Koh EK, Song SH, Sung JE, Lee HS, Hong JT, Hwang DY. Characterization of changes in global genes expression in the distal colon of loperamide-induced constipation SD rats in response to the laxative effectsof Liriope platyphylla. PloS One 2015. 10: e0129664.
    Pubmed KoreaMed CrossRef
  9. Lee E, Haa K, Yook JM, Jin MH, Seo CS, Son KH, Chang HW. Anti-asthmatic activity of an ethanol extract from Saururus chinensis. Biological and Pharmaceutical Bulletin 2006. 29: 211-215.
    Pubmed CrossRef
  10. Lee IS, Lee SO, Kim HS. Preparation and quality characteristics of yogurt added with Saururus chinensis (Lour.) Bail. Journal of the Korean Society of Food Science and Nutrition 2002. 31: 411-416.
    CrossRef
  11. Lee JH, Um YR, Shim KS, Jeon WK, Lee JH, Ma JY. Acute Toxicity Studyon FermentedSsanghwa-tang Extractsin Mice. KoreanJOrientIntMed 2009. 30: 780-787.
  12. Lee JJ, Lee YM, Jung SK, Kim KY, Lee MY. Effects of dietary mulberry leaf on loperamide-induced constipation in rats. Korean Journal of Food Preservation 2008. 15: 280-287.
  13. Lee KA, Kim HJ, Yoon HS. Constipation prevalence and lifestyle factors affecting constipation of high school students in Gimhae area. J Korean Diet Assoc 2009. 15: 419.
  14. Lee ST, Park JM, Lee HK, Kim MB, Cho JS, Heo JS. Component Comparison in Different Growth Stages and Organs of Saururus chinensis B(AILL). Korean Journal of Medicinal Crop Science 2000. 8: 312-318.
  15. Park CS, Park KS, Kim ML, Kong HJ, Yang KM. Effects of medicinal enzyme powder on intestinal mobility, lipid level, and blood parameters of loperamide-induced constipation in rats. Korean Society of Life Science 2013. 23: 228-236.
    CrossRef
  16. Park DJ, Lee JC. A Study on the Antioxidative and Depigmentation Activities of the Ethanol Extract of Saururus Herba1. Kor J Herbology 2008. 23: 193-202.
  17. Park MI, Shin JE, Myung SJ, Huh KC, Choi CH, Jung S, Choi MG. Guidelines for the treatment of constipation. The Korean Journal of Gastroenterology 2011. 57: 100-114.
    Pubmed CrossRef
  18. Shi N, Liu X, Guan Y. Research on k-means Clustering Algorithm: An Improved k-means Clustering Algorithm. 2010 Third International Symposium on Intelligent Information Technology and Security Informatics 2010. 63-67.
  19. Singh TR, Roy S, Singh OI, Sinam T, Singh K. A New Local Adap-tive Thresholding Technique in Binarization. International Journal of Computer Science Issues 2012. 8: 271-277.
  20. Shin OS, Shin HY, Lee KH, Kim GY, Kim KH, Park JK, Ahn JI, Song KY. Inhibition of Adipogenesis in 3T3-L1 Adipocytes with Ethanol Extracts of Saururus chinensis. Korean Society for Biotechnology and Bioengineering Journal 2012. 27: 381-386.
    CrossRef
  21. Wang XW, Liu HF, Xu M, Chen G, He JT, Wang GA, Teng XC, Fang DC. Proteomic analysis of down-regulated proteins in colonic mucosa of chronic slow transit constipation rats. Journal of Medical Colleges of PLA 2009. 24: 136-141.
    CrossRef
  22. You JS, Park JY, Chang KJ. Correlation among dietary habits score,life stress score and health-related quality of life (HRQL) scorefor female college students with functional constipation. Korean Journal of Nutrition 2010. 43: 620-627.
    CrossRef
  23. Zuo YM, Xu YL, Zhang ZL, Liu DH, Cai MT. Chemical Constituents from Saururus chinensis. Journal of Chinese Medicinal Materials 2015. 38: 2538-2540.
    Pubmed