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Cherry Tomato Supplementation Reduces Cardiovascular Risk
Biomed Sci Letters 2017;23:321-326
Published online December 31, 2017;
© 2017 The Korean Society For Biomedical Laboratory Sciences.

Jong-Ho Koh1, Young-Hee Lim2, and Dahyun Hwang3,4,†

1Department of Bio-Food Analysis, Bio-campus, Korea Polytechnics College, Chungnam 32943, Korea,
2Department of Public Health Science (BK21 PLUS Program), Graduate School, Korea University, Seoul 02841, Korea,
3Department of Biomedical Laboratory Science, College of Life and Health Sciences, Hoseo University, Chungnam 31499, Korea,
4The Research Institute for Basic Sciences, Hoseo University, Chungnam 31499, Korea
Correspondence to: Dahyun Hwang. Department of Biomedical Laboratory Science, Hoseo University, 20, Hoseo-ro 79 beon-gil, Baebang-eup, Asan-si, Chungcheongnam-do 31499, Korea. Tel: +82-41-540-9628, Fax: +82-41-540-9997, e-mail:
Received November 9, 2017; Revised December 12, 2017; Accepted December 12, 2017.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Tomatoes are rich in nutrients and have many beneficial advantages on human health. Four cherry tomato supplementation diets (CTSDs) were prepared from the juice and cake of fresh and processed (heat-treated) cherry tomatoes. Rats were fed CTSDs for 28 days and the changes in health indices in the serum were analyzed. CTSDs significantly decreased (P < 0.05) food efficiency ratio compared with the control. CTSD feeding significantly increased (P < 0.05) the high-density lipoprotein cholesterol level compared with the control, which resulted in a significant (P < 0.05) decrease in coronary artery risk index and atherogenic index. Furthermore, CTSD feeding increased serum serotonin level. These results indicate that CTSD shows antihyperlipidemic effect.

Keywords : Cherry tomato, Atherogenic index, Coronary artery risk index, Serotonin

The atherogenic index (AI) and the coronary artery risk index (CRI) are reliable indicators of the risk of cardiovascular diseases. An increase in the blood lipid level leads to hyperlipidemia which is the leading cause of cardiovascular disease and death worldwide. Increasing the high-density lipoprotein cholesterol (HDL-C) lowers the risk of cardiovascular diseases by reversing cholesterol transport, inhibiting low-density lipoprotein cholesterol (LDL-C) oxidation, and platelet aggregation (Barter, 2005; Berrougui et al., 2012). Food is an important factor to prevent hyperlipidemia in our daily life. Therefore, the intake of beneficial food for lowering the risk of hyperlipidemia is needed.

Tomato is a highly consumed vegetable worldwide and has plenty of health benefits. Tomatoes contain a high amount of fiber, oligosaccharides, and polysaccharides which act as prebiotic compounds in the gut environment (Bornet et al., 2002). Tomatoes reduce prostate and breast cancer risk, which might be related to the antioxidant properties of lycopene (Sato et al., 2002; Ilic and Misso, 2012). Lycopene in tomatoes showed the effect on protecting osteoporosis in postmenopausal women (Mackinnon et al., 2011). Nonetheless, there is limited information on the effects of cherry tomatoes (Solanum lycopersicum) on cardiovascular health. The aim of the present study was to evaluate the effects of cherry tomato supplementation (CTS) on reducing the risk of cardiovascular diseases in rats. Four types of cherry tomato supplementation diets (CTSDs) were prepared to investigate the beneficial effects of CTSDs on preventing cardiovascular disease.


Preparation and analysis of tomato samples

Cherry “Yoyo” tomatoes harvested from Chungcheongnam-do, South Korea were used in this study. Fresh and processed (heat-treated at 80°C for 15 min) cherry tomato samples were separated into juice and cake after processing with a pulper (AG-5500, Angel Juicer Co., Pusan, Korea). Fresh and heat-treated tomato juices were designated as J-1 and J-2, respectively. The tomato cake was treated with 1% Viscozyme® L (Novozymes Inc., Copenhagen, Denmark) (54:1, v/v) at 50°C for 4 h with shaking at 200 rpm, freeze-dried, and ground using a grinder (1093 Cyclote, Foss Tecator AB, Höganäs, Sweden). Fresh and heat-treated tomato cakes were designated as C-1 and C-2, respectively. The chemical composition of the cherry tomato powder was determined by the standard AOAC method (Cunniff, 1997). Lycopene was extracted from the cherry tomato powder and calculated based on a standard curve generated by a lycopene standard (Sigma, St Louis, MO, USA) (Koh et al., 2010). The chemical compositions of the cherry tomato powder is shown in Table 1.

Chemical compositions of cherry tomato sample

Protein (%)11.912.415.217.2
Lipid (%)
Ash (%)
Crude fiber (%)3.75.335.541.2
Lycopene (mg/100 g)180.0241.7130.2166.6


Male Sprague-Dawley rats (6 weeks old) were purchased from KOATECH (Gyeonggi-do, Pyeongtaek, Korea). They were housed and maintained at a constant temperature (24 ± 1°C) and humidity (55%) with 12 h cycles of light and dark. Rats were fed AIN-93G (TestDiet, St. Louis, MO, USA) for 7 days prior to experiments to allow adaptation, with free access to water. All experimental procedures were approved by the Korea University Institutional Animal Care and Use Committee (Approval No. KUIACUC-2013-151) and performed in accordance with the Guide for the Care and Use of Laboratory Animals (NIH Publication No. 85-23, 1996). Rats (n = 6) received pelleted CTSDs, which were prepared by Feedlab (Gyeonggi-do, Guri, Korea) to have the same chemical compositions (Table 2). Each CTSD was divided into two groups according to contents (6% and 12%) to investigate of concentration-dependent effect. Each CTSD was compensated to contain the same amount of protein (%), lipid (%), and crude fiber (%) using casein (protein), soybean oil (lipid), and cellulose (crude fiber). Corn starch was used to adjust the total amount of carbohydrate.

Composition of diet for rats used in this study

Composition(g/kg diet)J-1-6%J-1-12%J-2-6%J-2-12%C-1-6%C-1-12%C-2-6%C-2-12%Control
Corn starch347.8298.1350.4303.3371.4345.3376.8356.1397.5
Soybean oil69.068.067.765.366.563.065.761.470.0
Vitamin mix10.
Choline bitartrate2.


Measurement of health indices in serum

The changes in health indices in the serum of rats fed CTSDs for 4 weeks were analyzed. Food intake was measured daily, and body weights were determined weekly. Daily food intake, body weights, food efficiency ratio (FER), and relative liver weight were determined according to the previous reports (Kim et al., 2011; Jo et al., 2014). Serum alanine aminotransferase (AST), aspartate aminotransferase (ALT), glucose, TG, total cholesterol (TC), and HDL-C were measured using the FUSI DRI-CHEM SLIDE kit and a FUSI DRI-CHEM 4000 analyzer (Fujifilm, Tokyo, Japan). The serum LDL-C and very low-density lipoprotein cholesterol (VLDL-C) levels were calculated by applying Friedwann’s equation (Jo et al., 2014). The atherogenic index (AI) and the coronary artery risk index (CRI) were calculated according to the previous reports (Abbott et al., 1988; Adeneye et al., 2010).

Measurement of serotonin in serum

Serum serotonin (5-hydroxytryptamine) levels were measured using an enzyme-linked immunosorbent assay (ELISA; IBL Immuno Biological Lab, Hamburg, Germany) according to the manufacturer’s instructions.

Statistical analysis

All statistical analyses were performed using SPSS (SPSS, Chicago, IL, USA). The differences among groups were evaluated by analysis of variance (ANOVA), followed by Duncan’s multiple range tests. A level of P < 0.05 was considered statistically significant.


Chemical compositions analysis of CTSDs

The chemical compositions of CTSDs are shown in Table 1. To improve of bioavailability of tomatoes, we did two procedures such as heat treatment and enzyme treatment. Heating and fine grinding of foods could increase bioavailability by disrupting or softening the plant cell wall (Hussein and El-Tohamy, 1990). The use of multi-enzymatic complexes, such as Viscozyme-L, which contain cellulases, arabinases, hemicellulases, gluconases, and xylanases, causes the rupture of the cell walls, favoring the extraction of useful compounds (protein and sugars) from the vegetable tissues (Dueñas et al., 2007). As a result, heat treatment increased protein, lipid, crude fiber and lycopene in both juice and cake samples (Table 1).

Body weight, food intake and relative liver weight

Rats fed different CTSDs exhibited a significant decrease (P < 0.05) in food intake and body weight compared with the control (Table 3). However, the body weight was higher in the 12% supplemented CTSD than that in the 6% supplemented CTSD. Although all diets had the same amount of protein, lipid, and fiber, the FERs in all rats fed CTSDs significantly decreased (P < 0.05) compared with the control, and the FERs of the juice powder diet groups were higher than those of the cake powder diet groups. The FERs of rats fed different CTSDs were 8~34% lower than that of the control, which indicates that CTSDs might be useful for losing weight. Although the body weight and FER decreased in rats fed CTSDs, there were no changes in the relative liver weight.

Body weight, food intake, food efficiency ratio, and relative liver weight in rats fed different CTSDs for 4 weeks

GroupBody weight (g/day)Food intake (g/day)FER (%)Relative liver weight(g/100 g body weight)
J1-6%3.31 ± 0.16ab15.92 ± 0.11ab0.21 ± 0.00a (72.12)2.93 ± 0.45a
J1-12%3.48 ± 0.11bc15.25 ± 0.42a0.23 ± 0.01ab (79.12)2.98 ± 0.09a
J2-6%3.76 ± 0.19c15.57 ± 0.06ab0.24 ± 0.00bc (83.75)3.12 ± 0.17a
J2-12%4.17 ± 0.29d15.88 ± 0.79ab0.26 ± 0.01cd (91.24)3.08 ± 0.16a
C1-6%3.09 ± 0.15a14.74 ± 0.96a0.21 ± 0.01a (72.80)3.02 ± 0.32a
C1-12%3.10 ± 0.06a14.79 ± 0.47a0.21 ± 0.01a (72.67)2.97 ± 0.20a
C2-6%3.63 ± 0.23bc15.16 ± 0.95a0.24 ± 0.01bc (83.23)3.02 ± 0.22a
C2-12%3.61 ± 0.06bc15.11 ± 0.55a0.24 ± 0.01bc (83.05)2.86 ± 0.28a
Control4.99 ± 0.22e17.38 ± 1.51b0.29 ± 0.02d (100.00)2.92 ± 0.07a

The control diet consisted of AIN-93G. Values were expressed as a mean ± SD of three independent experiments. The FERs of the samples were normalized by taking the FER of the control to be 100% and are shown in parentheses.

abcdeValues in the same column not sharing a common superscript are significantly different (P < 0.05) from each other.

Serum analysis

At the end of the 4-week experiment, AST and ALT values in rats fed CTSDs were not significantly different from those in the control, indicating the absence of hepatotoxicity (Table 4). The glucose levels were significantly reduced (P < 0.05), except in the 6%-J-1 group, and the reduction was more significant in the high CTSD group than in the low CTSD group, except in the C-2 group. The TG levels were significantly reduced (P < 0.05), except in the J-2 group. Different CTSDs significantly increased (P < 0.05) the HDL-C level compared with the control. The HDL-C level was higher in rats fed the 12% supplemented diet than those fed the 6% supplemented diet. In the 12%-J-2 group, the HDL increased 1.47-fold compared with the control. However, there was no significant effect of CTSD on the reduction of TC and LDL-C. Treatment of CTDSs significantly (P < 0.05) decreased the AI and CRI compared with the control, and the AI and CRI were 1.1- to 1.8-fold and 1.1- to 1.3-fold reduction, respectively. Tomato supplementation decreased serum lipids (TC, LDL-C, and TG) levels in rats fed a high cholesterol diet (Nouri and Abad, 2013); however, in the present study, TC level was not lowered in the rats fed CTDS compared with the control. The AI and CRI are predicators of cardiovascular risk, which means that an elevation of AI and CRI indicates an increase in cardiovascular risk. A high level of cardiovascular risk results in atherosclerosis and coronary artery disease (Hokanson and Austin, 1996; Fki et al., 2005). A high AI and CRI correlate with low HDL-C and high TC levels or the LDL-C level and vice versa. In the present study, although the HDL-C level in CTSD-fed rats increased significantly, there was no significant reduction in the TC and LDL-C levels in CTSD-fed rats. Therefore, the significant reduction of AI and CRI in the rats fed CTDS was mainly caused not by a decrease in the levels of TC, LDL-C, and VLDL-C but by a significant increase in the HDL-C level.

Serum analysis of collected blood of rats fed different CTSDs for 4 weeks

GroupAST (U/L)ALT (U/L)Glucose (U/L)TG (U/L)TC (mg/dL)LDL-C (mg/dL)HDL-C (mg/dL)VLDL-C (mg/dL)AICRI
J1-6%87.00 ± 5.66a26.50 ± 0.71ab116.33 ± 10.97a45.00 ± 3.61abc57.33 ± 4.04a9.13 ± 1.70a44.00 ± 1.00a10.13 ± 0.76a0.21 ± 0.05ab1.30 ± 0.06b
J1-12%83.00 ± 4.24a33.00 ± 4.24b102.33 ± 5.69ab37.33 ± 6.11ab59.00 ± 7.94a10.87 ± 0.83ab47.33 ± 1.15ab9.80 ± 0.87a0.23 ± 0.03ab1.25 ± 0.02a
J2-6%91.00 ± 1.41a26.50 ± 0.71ab110.33 ± 8.50a67.67 ± 17.62d71.33 ± 4.04b11.00 ± 2.12ab49.33 ± 2.52abc21.20 ± 1.60b0.22 ± 0.02ab1.45 ± 0.04c
J2-12%82.50 ± 9.19a24.00 ± 5.66a92.33 ± 9.29b56.33 ± 10.79cd68.67 ± 8.08ab14.40 ± 2.78bc55.67 ± 0.58d15.13 ± 2.52c0.26 ± 0.04ab1.23 ± 0.07a
C1-6%82.50 ± 6.36a22.00 ± 5.66a103.33 ± 2.08ab35.00 ± 6.93b60.00 ± 1.73ab11.13 ± 2.20ab46.67 ± 2.52ab8.87 ± 1.36a0.24 ± 0.03ab1.29 ± 0.05ab
C1-12%88.50 ± 2.12a29.50 ± 4.95ab102.00 ± 8.19ab32.67 ± 3.79b64.00 ± 7.94ab15.87 ± 4.24c47.67 ± 4.51ab8.93 ± 1.42a0.33 ± 0.06c1.34 ± 0.02b
C2-6%78.00 ± 7.07a21.00 ± 1.41a93.67 ± 3.79b31.33 ± 8.08b67.67 ± 8.02ab14.27 ± 1.97bc54.00 ± 4.00cd8.47 ± 0.50a0.26 ± 0.05ab1.25 ± 0.02a
C2-12%81.00 ± 7.07a27.50 ± 0.71ab104.33 ± 4.04ab29.00 ± 5.57b63.00 ± 5.29ab18.13 ± 2.01c51.33 ± 3.79bcd8.67 ± 1.67a0.35 ± 0.02c1.23 ± 0.07a
Control92.50 ± 9.19a24.50 ± 0.71ab116.00 ± 9.17a51.33 ± 3.51ac61.00 ± 4.58ab14.27 ± 2.64bc38.00 ± 4.00e13.07 ± 2.90c0.38 ± 0.13cd1.61 ± 0.09d

Values were expressed as a mean ± SD of three independent experiments.

abcdeValues in the same column not sharing a common superscript are significantly different (P < 0.05) from each other.

To explain changes in eating behavior, meal size, and body weight by feeding CTSD, the serum serotonin level was measured. Serum serotonin levels significantly increased (P < 0.05) in a dose-dependent manner in rats fed different CTSDs, except in the C-2 group (Table 5). Diet influences the serotonergic system, and horses fed a high fiber diet show significantly higher serotonin levels than those fed a high starch diet (Alberghina et al., 2010). Serotonin plays an important role in feeding and appetite control, and a lack of serotonin can increase food intake (Tecott et al., 1995; Leibowitz and Alexander, 1998). In this study, CTSDs increased serotonin, which might affect the reduction of food intake and body weight gain.

Serum serotonin concentration of rats fed different CTSDs for 4 weeks

GroupSerotonin (ng/mL)
J-1-6%3.50 ± 0.03ab
J-1-12%3.55 ± 0.02ab
J-2-6%3.50 ± 0.08ab
J-2-12%3.51 ± 0.01ab
C-1-6%3.47 ± 0.01ad
C-1-12%3.50 ± 0.04ab
C-2-6%3.52 ± 0.02ab
C-2-12%3.30 ± 0.01c
Control3.42 ± 0.04d

Values were expressed as a mean ± SD of three independent experiments.

abcdValues in the same column not sharing a common superscript are significantly different (P < 0.05) from each other.

In conclusion, CTSD feeding lowered FER, and significantly increased HDL-C, resulted in AI and CRI reduction. In addition, CTSD increased serum serotonin level. Therefore, a constant intake of cherry tomato might reduce the potential risk of cardiovascular disease in healthy individuals as well as in individuals with hyperlipidemia.



  1. Abbott RD, Wilson PW, Kannel WB, and Castelli WP. High density lipoprotein cholesterol, total cholesterol screening, and myocardial infarction. The Framingham study. Arteriosclerosis, Thrombosis, and Vascular Biology 1988;8:207-211.
  2. Adeneye AA, Adeyemi OO, and Agbaje EO. Anti-obesity and antihyperlipidaemic effect of hunteria umbellata seed extract in experimental hyperlipidaemia. Journal of Ethnopharmacology 2010;130:307-314.
    Pubmed CrossRef
  3. Alberghina D, Giannetto C, Visser E, and Ellis A. Effect of diet on plasma tryptophan and serotonin in trained mares and geldings. Veterinary Record 2010;166:133-136.
    Pubmed CrossRef
  4. Barter P. The role of HDL-cholesterol in preventing atherosclerotic disease. European Heart Journal Supplements 2005;7:F4-F8.
  5. Berrougui H, Momo CN, and Khalil A. Health benefits of high-density lipoproteins in preventing cardiovascular diseases. Journal of Clinical Lipidology 2012;6:524-533.
    Pubmed CrossRef
  6. Bornet F, Brouns F, Tashiro Y, and Duvillier V. Nutritional aspects of short-chain fructooligosaccharides: natural occurrence, chemistry, physiology and health implications. Digestive and Liver Disease 2002;34:S111-S120.
  7. Cunniff P. AOAC international: Gaithersburg 1997.
  8. Due챰as M, Hern찼ndez T, and Estrella I. Changes in the content of bioactive polyphenolic compounds of lentils by the action of exogenous enzymes. Effect on their antioxidant activity. Food Chemistry 2007;101:90-97.
  9. Fki I, Bouaziz M, Sahnoun Z, and Sayadi S. Hypocholesterolemic effects of phenolic-rich extracts of chemlali olive cultivar in rats fed a cholesterol-rich diet. Bioorganic and Medicinal Chemistry 2005;13:5362-5370.
    Pubmed CrossRef
  10. Hokanson JE, and Austin MA. Plasma triglyceride level is a risk factor for cardiovascular disease independent of high-density lipoprotein cholesterol level: A metaanalysis of population-based prospective studies. Journal of Cardiovascular Risk 1996;3:213-219.
    Pubmed CrossRef
  11. Hussein L, and El-Tohamy M. Vitamin a potency of carrot and spinach carotenes in human metabolic studies. International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin-und Ernahrungsforschung. Journal International De Vitaminologie Et De Nutrition 1990;60:229-235.
  12. Ilic D, and Misso M. Lycopene for the prevention and treatment of benign prostatic hyperplasia and prostate cancer: A systematic review. Maturitas 2012;72:269-276.
    Pubmed CrossRef
  13. Jo SP, Kim JK, and Lim YH. Antihyperlipidemic effects of stilbenoids isolated from morus alba in rats fed a high-cholesterol diet. Food and Chemical Toxicology 2014;65:213-218.
    Pubmed CrossRef
  14. Kim KY, Park CW, Kim HM, Kang IC, and Jeong HJ. Effects of acanthopanax divaricatus var. Albeofructus in mice submitted to protein restriction. Oriental Pharmacy and Experimental Medicine 2011;11:15-23.
  15. Koh JH, Kim Y, and Oh JH. Chemical characterization of tomato juice fermented with bifidobacteria. Journal of Food Science 2010;75:428-432.
    Pubmed CrossRef
  16. Leibowitz SF, and Alexander JT. Hypothalamic serotonin in control of eating behavior, meal size, and body weight. Biological Psychiatry 1998;44:851-864.
  17. Mackinnon E, Rao A, Josse R, and Rao L. Supplementation with the antioxidant lycopene significantly decreases oxidative stress parameters and the bone resorption marker n-telopeptide of type i collagen in postmenopausal women. Osteoporosis International 2011;22:1091-1101.
    Pubmed CrossRef
  18. Nouri MHK, and Abad ANA. Comparative study of tomato and tomato paste supplementation on the level of serum lipids and lipoproteins levels in rats fed with high cholesterol. Iranian Red Crescent Medical Journal 2013;15:287.
    Pubmed KoreaMed CrossRef
  19. Sato R, Helzlsouer KJ, Alberg AJ, Hoffman SC, Norkus EP, and Comstock GW. Prospective study of carotenoids, tocopherols, and retinoid concentrations and the risk of breast cancer. Cancer Epidemiology and Prevention Biomarkers 2002;11:451-457.
  20. Tecott LH, Sun LM, Akana SF, Strack AM, Lowenstein DH, Dallman MF, and Julius D. Eating disorder and epilepsy in mice lacking 5-ht2c serotonin receptors. Nature 1995;374:542-546.
    Pubmed CrossRef