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Effect of the Ag3PO4 on Staphylococcus aureus Growth and Human Immunity
Biomed. Sci. Lett. 2018;24:30-34
Published online March 31, 2018;  https://doi.org/10.15616/BSL.2018.24.1.30
© 2018 The Korean Society For Biomedical Laboratory Sciences.

Mi Kyung Kim1,† and Dae-Sik Kim2

1Department of Systems Biology, College of Life Science, Yonsei University, Seoul 03722, Korea 2Department of Clinical Laboratory Science, Dongnam Health University, Suwon 16328, Korea
Correspondence to: Mi Kyung Kim. Department of Systems Biology, College of Life Science, Yonsei University, Seoul 03722, Korea. Tel: +82-2-2123-2709, Fax: +82-2-312-5657, e-mail: biokyung@gmail.com
Received January 3, 2018; Revised March 12, 2018; Accepted March 13, 2018.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
 Abstract
Silver (Ag) has been widely used in commercial products and medical fields since ancient times because of its antibacterial effect. It is harmless and non-toxic to the human body. For this reason, recent research has actively evaluated antimicrobial activity using silver (Ag). In this study, we investigated the inhibitory effect of a silver-based compound, silver phosphate (Ag3PO4) on the growth of Staphylococcus aureus and the activation of human immunity. First, the inhibitory effect of Ag3PO4 on the growth of Staphylococcus aureus was confirmed by a growth curve and a colony-ounting method. As a result, the growth inhibitory effect increased as the concentration of Ag3PO4 increased. Specifically, treatment with 5 μg/mL of Ag3PO4 resulted in no bacteria growth, and the colony-counting method showed a remarkable inhibition. In addition, the expression of cytokine IL-8 by Ag3PO4 was examined to investigate the cellular immune system activation by Ag3PO4. After pretreatment of Staphylococcus aureus for 1 hour with 50 μg/mL Ag3PO4, an increased IL-8 mRNA expression resulted. In cells treated with Ag3PO4, we found that the expression of IL-8 was enhanced in a time-dependent fashion compared to non-treated cells. These results indicate that Ag3PO4 induces antimicrobial activity against Staphylococcus aureus and activates human immunity. These results are expected to contribute to the future study of the mechanism of silver (Ag) and silver-based compounds in relation to antibacterial activity.
Keywords : Silver, Staphylococcus aureus, Antibacterial, Immunity