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The Pro-apoptotic Effects of S100A8 and S100A9 in Human Monocytic Leukemia Cells, THP-1
Biomed Sci Letters 2018;24:134-137
Published online June 30, 2018;  https://doi.org/10.15616/BSL.2018.24.2.134
© 2018 The Korean Society For Biomedical Laboratory Sciences.

In-Sik Kim1,2 and Ji-Sook Lee3,†

1Department of Senior Healthcare, BK21 Plus Program, Graduate School, Eulji University, Daejeon 34824, Korea
2Department of Biomedical Laboratory Science, School of Medicine, Eulji University, Daejeon 34824, Korea
3Department of Clinical Laboratory Science, Wonkwang Health Science University, Iksan 54538, Korea
Correspondence to: Ji-Sook Lee. Department of Clinical Laboratory Science, Wonkwang Health Science University, 501, Iksandaero, Iksan 54538, Korea.
Tel: +82-63-840-1216, Fax: +82-63-840-1219, e-mail: jslee1216@wu.ac.kr
Received May 17, 2018; Accepted May 28, 2018.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
 Abstract
S100A8 and S100A9 are involved in pathogenesis of cancer by induction or inhibition of cancer as well as inflammation. In this study, we investigated the association of S100A8 and S100A9 with pathogenesis of leukemia using human monocytic leukemia cells, THP-1. The expression of TLR4, which is a known receptor of S100A8 and S100A9, was examined by using flow cytometry and Western blotting. THP-1 cells have high surface and cytosol expression of TLR4. S100A8 and S100A9 suppressed the cell survival, and this suppression was found to be associated with apoptosis because they increased the number of apoptotic cells in a dose- and a time-dependent manners. However, S100A8 and S100A9 had no effect on the survival and apoptosis of monocytes isolated from the peripheral blood. We next examined the apoptotic effect of lipopolysaccharide (LPS) and monophosphoryl lipid A (MPLA), which are other ligands of TLR4, in THP-1 cells. Lipopolysaccharide had no effect on cell survival, but MPLA is effective on the cell apoptosis. These results suggest that S100A8 and S100A9 may regulate leukemia cell survival via TLR4, which is an essential receptor in the pro-apoptotic mechanism induced by S100A8 and S100A9. These findings may shed light on development of a possible therapeutic drug for leukemia treatment.
Keywords : S100, Apoptosis, Leukemia