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Genetic Variations of Trichophyton rubrum Clinical Isolates from Korea
Biomed. Sci. Lett. 2018;24:221-229
Published online September 30, 2018;  https://doi.org/10.15616/BSL.2018.24.3.221
© 2018 The Korean Society For Biomedical Laboratory Sciences.

Nam-Sup Yoon1,2,짠,*, Hyunjung Kim3,**, Sung-Bae Park4,5,***, Min Park6,****, Sunghyun Kim4,5,,**** and Young-Kwon Kim2,,****

1Department of Laboratory Medicine, Asan Medical Center, Seoul 05505, Korea
2Department of Biomedical Laboratory Science, College of Medical Sciences, Konyang University, Daejeon 35365, Korea
3QuantaMatrix, Inc., Seoul 03082, Korea
4Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan, Busan 46252, Korea
5Clinical Trial Specialist Program for in vitro Diagnostics, Brain Busan 21 Plus Program, Graduate School, Catholic University of Pusan, Busan 46252, Korea
6Department of Biomedical Laboratory Science, Daekyung University, Gyeongsan 38547, Korea
Correspondence to: Sunghyun Kim. Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan, Busan46252, Korea.
Tel: +82-51-510-0560, Fax: +82-51-510-0568, e-mail: shkim0423@cup.ac.kr
Young-Kwon Kim. Department of Biomedical Laboratory Science, College of Medical Sciences, Konyang University, Daejeon 35365, Korea.
Tel: +82-42-660-6371, Fax: +82-42-543-6370, e-mail: ykkim3245@konyang.ac.kr
*Medical technologist, **Research scientist, ***Graduate student, ****Professor.
Equal contributors.
Received August 17, 2018; Revised September 13, 2018; Accepted September 13, 2018.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
 Abstract
Trichophyton rubrum is one of the well-known pathogenic fungi and causes dermatophytosis and cutaneous mycosis in human world widely. However, there are not an available sequence type (ST) classification methods and previous studies for T. rubrum until now. Therefore, currently, molecular biological tools using their DNA sequences are used for genotype identification and classification. In the present study, in order to characterize the genetic diversity and the phylogenetic relation of T. rubrum clinical isolates, five different housekeeping genes, such as actin (ACT), calmodulin (CAL), RNA polymerase II (RPB2), superoxide dismutase 2 (SOD2), and 棺-tubulin (BT2) were analyzed using by multi-locus sequence typing (MLST). Also, DNA sequence analysis was performed to examine the differences between the sequences of Trichophyton strains and the identified genetic variations sequence. As a result, most of the sequences were shown to have highly matched rates in their housekeeping genes. However, genetic variations were found on three different positions of 棺-tubulin gene and were shown to have changed from C넂G (1766), G넂T (1876), and C넂A (1886). To confirm the association with T. rubrum inheritance, a phylogenetic tree analysis was performed. It was classified as four clusters, but there was little significant correlation. Even so, MLST analysis is believed to be helpful for determining the genetic variations of T. rubrum in cases where there is more large-scale data accumulation. In conclusion, the present study demonstrated the first MLST analysis of T. rubrum in Korea and explored the possibility that MLST could be a useful tool for studying the epidemiology and evolution of T. rubrum through further studies.
Keywords : Trichophyton rubrum, Multi-locus sequence typing, Genetic variations, Clinical isolates