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Immunofluorescence Microscopic Evaluation of Tight Junctional Proteins during Enterotoxigenic Bacteroides fragilis (ETBF) Infection in Mice
Biomed. Sci. Lett. 2018;24:275-279
Published online September 30, 2018;  https://doi.org/10.15616/BSL.2018.24.3.275
© 2018 The Korean Society For Biomedical Laboratory Sciences.

Soonjae Hwang1,*, Yeowool Kang1,*, Minjeong Jo1,*, Sung Hoon Kim1,*, Won Gil Cho2,** and Ki-Jong Rhee1,,**

1Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University at Wonju, Wonju, Gangwon-do 26493, Korea
2Department of Anatomy, Wonju College of Medicine, Yonsei University at Wonju, Wonju, Gangwon-do 26493, Korea
Correspondence to: Ki-Jong Rhee. Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University at Wonju, Wonju, Gangwon-do 26493, Korea.
Tel: +82-33-760-2445, Fax: +82-33-760-2195, e-mail: kjrhee@yonsei.ac.kr
*Graduate student, **Professor.
Received August 17, 2018; Accepted August 30, 2018.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
 Abstract
Inflammatory bowel disease (IBD) is increasing in prevalence in developed countries but the cause of this increase is unclear. In animal models of IBD and in human IBD patients, alterations in the tight junctional proteins have been observed, suggesting that the intestinal microflora may penetrate the underlying colonic tissue and promote inflammation. Enterotoxigenic Bacteroides fragilis (ETBF) causes inflammatory diarrhea in human and is implicated in inflammatory bowel diseases. However, it is unclear whether alterations in tight junctional proteins occur during ETBF infection in mice. In this brief communication, we report that ETBF infection induces up-regulation of claudin-2 and down-regulation of claudin-5 through B. fragilis toxin (BFT) activity in the large intestine of C57BL/6 mice. In contrast, BFT did not induce changes in tight junctional proteins in the HT29/C1 cell line, suggesting that analysis of biological activity of BFT in vivo is important for evaluating ETBF effects.
Keywords : Enterotoxigenic Bacteroides fragilis, Tight junction, Claudin, Bacteroides fragilis toxin