Experimental dosage of treated drugs and herbal black vinegar (HBV) used for HFD
| Group | Diets | Dose (mg/kg/day) | Treatment drug |
|---|---|---|---|
| Control | Norma pellet | 10 mL/kg | Vehicle (distilled water) |
| HFD pellet | 10 mL/kg | Vehicle | |
| References | HFD pellet | 100 mg/kg | Silymarin 100 mg in 10 mL of distilled water |
| HFD pellet | 250 mg/kg | Metformin 250 mg in 10 mL of distilled water | |
| Active | HFD pellet | Stock | HBV stock solution 10 mL |
| HFD pellet | 2-fold dilution | HBV 2-fold dilution solution 10 mL | |
| HFD pellet | 4-fold dilution | HBV 4-fold dilution solution 10 mL | |
Abbreviations: HFD, high fat diet; HBV: herbal black vinegar
Seven groups (total 56 adapted mice on HFD were selected base on the body weight at 7 days after initiation of HFD supply, and 2 mice in each group were removed based on the standard deviation of body weight at end of 84 days of administrations or mortalities; finally 6 mice per groups, total 42 mice were sacrificed) were used in this experiment. All test materials and two different reference drugs were directly diluted or dissolved in distilled water, and administered in a volume of 10 mL/kg, once a day for 84 days from 1 week after HFD supply
