Biomedical Science Letters

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Fig. 3. Sample and artificial infection tests of developed RT-nested PCR primer set for the detection of HuSaV in this study. (A) HuSaV detection using the composition including RT-nested PCR primer sets and conditions developed in this study. (B) Artificial infection tests of 20 groundwater samples. M, 100 bp Ladder marker (Enzynomics); -1 ~ -7, template dilution value from 1 ng/μL HuSaV plasmid; N, negative control, PN, PCR negative control.
Biomed Sci Letters 2021;27:35-43
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