Biomedical Science Letters

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Fig. 4. The positive control design information and restriction enzyme treatment result developed in this study. (A) Design information of positive control capable of false positive test via restriction enzyme cutting from nested PCR product and amplicon size thereof. (B) Restriction enzyme EcoRV digestion of plasmid and development of positive control using the nested PCR amplicons as a template. M, 100 bp DNA Ladder marker (Enzynomics); P, plasmid; dP, developed positive control; N, negative control; PN, PCR negative control; Pt, plasmid treatment; dPt, developed positive control treatment.
Biomed Sci Letters 2021;27:35-43
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