Biomedical Science Letters

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Fig. 1. S100A8 and S100A9 suppress the expression and phosphorylation of PDGFRα in EoL-1 cells. (A). The total RNAs from EoL-1 cells were collected, and RT-PCR was conducted to detect the FIP1L1-PDGFRα gene. (B) EoL-1 cells were incubated with S100A8 and S100A9 at the indicated concentrations for the indicated time. The survival rate of the cells was evaluated using an MTT assay. *P < 0.05 and **P < 0.01 indicate a significant difference between the control and stimulator-treated groups. (C and D) EoL-1 cells were pretreated without (C) or with (D) cycloheximide (CHX) and then incubated with S100A8 and S100A9 (10 μg/mL) for the indicated time. Expression of the indicated proteins was evaluated using Western blotting. μ-actin was used as the internal control.
Biomed Sci Letters 2021;27:95-8
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