Biomedical Science Letters

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Fig. 3. I3M suppresses JNK1 activity. (A) Chemical structure of I3M. (B) After transiently transfected with plasmids expressing pcDNA3-HA-JNK1 at HEK 293 cells and then treated with various concentrations (5, 10, 100, 500, 10,000, and 100,000 nM) of I3M for 4 h. The cells were lysed and then immunoprecipitated using anti-HA antibody, after 48 h of transfection. The immunopellets were incubated with purified GST-c-Jun, then assayed for JNK1 activity.
Biomed Sci Letters 2021;27:134-41
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