Fig. 4. Quantification of the mRNA profile of up-regulated genes in RNA-seq. SW620 cells incubated with each concentration of TTX (0 and 100 μM) for 48 h. TTX exposure affected the mRNA abundance of certain genes in the cells. The mRNA level of both genes was normalized to the GAPDH gene. Data are expressed as the mean ± SEM. All experiments were repeated at least three times. ***P<0.001 vs control.
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