RT/Nested PCR primer sets of
Virus | Target gene | Type | Name | Sequence (5'→3') | Polarity | Location | Size (bp) | Remark |
---|---|---|---|---|---|---|---|---|
NS7-VP1 | RT-PCR | SV-F13 | GAY YWG GCY CTC GCY ACC TAC | + | 5074~5094 | 803 | KP298674.1 | |
RT-PCR | SV-R13 | GGT GAN AYN CCA TTK TCC AT | - | 5857~5876 | ||||
Nested PCR | SV-F21 | ANT AGT GTT TGA RAT GGA GGG | + | 5157~5177 | 435 | |||
Nested PCR | SV-R2 | GWG GGR TCA ACM CCW GGT GG | - | 5572~5591 | ||||
VP1 | RT-PCR | GI-F1M | CTG CCC GAA TTY GTA AAT GAT GAT | + | 5339~5362 | 330 | JQ388274.1 | |
RT-PCR | GI-R1M | CCA ACC CAR CCA TTR TAC ATY TG | - | 5646~5668 | ||||
Nested PCR | GI-F2-U | TGA TGG CGT CTA AGG ACG C |
+ | 5355~5377 | 314 | |||
Nested PCR | GI-R1M | CCA ACC CAR CCA TTR TAC ATY TG | - | 5646~5668 | ||||
NS7-VP1 | RT-PCR | GII-F1M | GGG AGG GCG ATC GCA ATC T | + | 5058~5076 | 341 | KX356908.1 | |
RT-PCR | GII-R1M | CCR CCI GCA TRI CCR TTR TAC AT | - | 5376~5398 | ||||
Nested PCR | GII-F3M | TTG TGA ATG AAG ATG GCG TCG ART | + | 5088~5111 | 311 | |||
Nested PCR | GII-R1M-U | GCC RCC IGC ART ICC RTT RTA CAT |
- | 5376~5398 |
1) Universal Primer (T7), AATACGACTCACTATAG (17 bp); 2) Universal Primer (M13R), GCGGATAACAATTTCACACAGG (22 bp)