Biomedical Science Letters

Table. 1. Table 1

RT/Nested PCR primer sets of Sapovirus and Norovirus GI · GII

Virus	Target gene	Type	Name	Sequence (5'→3')	Polarity	Location	Size (bp)	Remark
Sapovirus	NS7-VP1	RT-PCR	SV-F13	GAY YWG GCY CTC GCY ACC TAC	+	5074~5094	803	KP298674.1
		RT-PCR	SV-R13	GGT GAN AYN CCA TTK TCC AT	-	5857~5876
		Nested PCR	SV-F21	ANT AGT GTT TGA RAT GGA GGG	+	5157~5177	435
		Nested PCR	SV-R2	GWG GGR TCA ACM CCW GGT GG	-	5572~5591
Norovirus GI	VP1	RT-PCR	GI-F1M	CTG CCC GAA TTY GTA AAT GAT GAT	+	5339~5362	330	JQ388274.1
		RT-PCR	GI-R1M	CCA ACC CAR CCA TTR TAC ATY TG	-	5646~5668
		Nested PCR	GI-F2-U	¹⁾AAT ACG ACT CAC TAT AGA TGA TGA TGG CGT CTA AGG ACG C	+	5355~5377	314
		Nested PCR	GI-R1M	CCA ACC CAR CCA TTR TAC ATY TG	-	5646~5668
Norovirus GII	NS7-VP1	RT-PCR	GII-F1M	GGG AGG GCG ATC GCA ATC T	+	5058~5076	341	KX356908.1
		RT-PCR	GII-R1M	CCR CCI GCA TRI CCR TTR TAC AT	-	5376~5398
		Nested PCR	GII-F3M	TTG TGA ATG AAG ATG GCG TCG ART	+	5088~5111	311
		Nested PCR	GII-R1M-U	²⁾GCG GAT AAC AAT TTC ACA CAG GCC RCC IGC ART ICC RTT RTA CAT	-	5376~5398

¹⁾ Universal Primer (T7), AATACGACTCACTATAG (17 bp); ²⁾ Universal Primer (M13R), GCGGATAACAATTTCACACAGG (22 bp)