Fig. 2. Peak calling using MACS2 and SICER tools in public histone H3K27ac and H3K27me3 ChIP-seq data.
(A) H3K27ac and H3K27me3 peaks were identified using peak caller MACS2 and SICER with three analysis methods. (B) Peaks identified by each method were presented in genome browser of H3K27ac and H3K27me3 using different colors. Length of peaks (C) and counts per million (CPM) values of peaks (D) were graphed in boxplot. Box and whiskers represent interquartile range (IQR) and ±1.5×IQR values from edge of the box, respectively. (E) Signal-to-background ratio (S/B ratio) was calculated by analyzing H3K27ac or H3K27me3 level at each peak. (F) The S/B ratio of H3K27ac or H3K27me3 peaks was compared between three analysis methods. Difference of the S/B ratio was indicated in multiples.
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