Biomedical Science Letters

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Fig. 4. Analysis of histone H3K27ac and H3K27me3 ChIP-seq data in different sequencing depth. H3K27ac and H3K27me3 ChIP-seq data of control K562 cells (Con) were re-generated with 15, 30, 60 or 120 million (M) read counts. (A) The total read counts of Con ChIP-seq data were listed in a table. (B) Distribution of histone H3K27ac and H3K27me3 and peaks were visualized in genome browser with different read counts. Newly identified peaks in 120 M for H3K27ac were highlighted with red shadow. (C) The number of identified peaks was graphed in 15 M, 30 M, 60 M and 120 M. (D) The S/B ratio of peaks was compared with different read counts. Difference of the S/B ratio was indicated in multiples. Histone H3K27ac (E) and H3K27me3 peaks (F) identified in different read counts were overlapped and visualized by venn diagram (left). The number of peaks was graphed according to the length of them (right).
Biomed Sci Letters 2022;28:109-19
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