Sequence of primers used for RT-PCR
| Gene | Primer | Oligonucleotide sequence (5'-3') |
|---|---|---|
| GAPDH | F | 5'CAATGAATACGGCTACAGCAAC3' |
| R | 5'AGGGAGATGCTCAGTGTTGG3' | |
| iNOS | F | 5'CCCTTCCGAAGTTTCTGGCAGCAGC3' |
| R | 5'GGCTGTCAGAGCCTCGTGGCTTTGG3' | |
| COX-2 | F | 5'TCTCAGCACCCACCCGCTCA3' |
| R | 5'GCCCCGTAGACCCTGCTCGA3' | |
| IL-1β | F | 5'CAGGGTGGGTGTGCCGTCTTTC3' |
| R | 5'TGCTTCCAAACCTTTGACCTGGGC3' | |
| TNF-α | F | 5'TTGACCTCAGCGCTGAGTTG3' |
| R | 5'CCTGTAGCCCACGTCGTAGC3' | |
| IL-6 | F | 5'GTACTCCAGAAGACCAGAGG3' |
| R | 5'TGCTGGTGACAACCACGGCC3' |
