Fig. 2. Inhibition of nitric oxide (NO) and cell Viability by ASE.
MH-S cells were preincubated with varying doses of ASE for 30 min and then stimulated with CFA for 18 h. Cell supernatant was then mixed with equal amounts of Griess reagent and NO production was measured (A). Effects of ASE on cell viability were measured by MTT assay (B). Values in the bar graph are mean ± SEM of at least 3 independent experiments. ***P < 0.001, and *P < 0.05 when compared to CFA only treatment.
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