Biomedical Science Letters

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Fig. 3. Reverse-transcriptase polymerase chain reaction for pro-inflammatory mediators and cytokines by ASE. MH-S cells were seeded into 6-well plates for 24 h, treated with or without ASE at and then treated with or without CFA 30 min later. RNA was extracted 18 h later using TRIZOL solution and RT-PCR was carried out. iNOS, COX-2, TNF-α, IL-1β, IL-6, and GAPDH expression was determined (A). Gel image quantification for relative (B) iNOS, (C) COX-2, (D) TNF-α, (E) IL-1β, and (F) IL-6 expression against GAPDH was carried out in triplicates using the ImageJ software. Values in the bar graph are mean ± SEM of at least 3 independent experiments. *P < 0.05 when compared to CFA only treatment. B; Basal, CFA; Coal Fly Ash.
Biomed Sci Letters 2022;28:170-7 https://doi.org/10.15616/BSL.2022.28.3.170
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