Biomedical Science Letters

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Fig. 2. BEAS-2B cell supernatants treated with TNF-α, IFN-γ and NRG-1 suppresses anti-apoptotic effect of the supernatants exposed to TNF-α and IFN-γ on neutrophils. (A) Neutrophils (n=3) and eosinophils (n=3) were isolated and incubated for 24 h or 48 h in the absence or presence of TNF-α+and IFN-γ (10 ng/mL) or/and NRG-1 (10 nmol/mL). (B) BEASE-2B cells were treated with TNF-α+IFN-γ (10 ng/mL) or/and NRG-1 (10 nmol/mL). The supernatants were collected and used for treatment of neutrophils (n=3) and eosinophils (n=3). The supernatant-treated cells were incubated for 24 h (neutrophils) or 48 h (eosinophils) Apoptotic cells were evaluated using annexin-PI staining. Data are presented as mean ± SD relative to the control, which was set to be 100%. **, P < 0.01 and *, P < 0.05, significant differences between non-treated and stimulator-treated groups.
Biomed Sci Letters 2022;28:192-4
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