Fig. 2. The three extracts enhances the natural cytotoxicity of NK cells.
PBMCs exposed at varying concentrations for 20 h and then mixed with K562. After 2 h incubation at 37℃, cytotoxic degranulation of NK cells was measured by cell surface expression of CD107a on CD3-CD56+ NK cells. (A) The expression of CD107a of the Saussurea neoserrata Nakai, (B) The expression of CD107a of the Nypa fruticans Wurmb, (C) The expression of CD107a of the Calystegia dahuricus (Herb.) Choisy. (D) The combination treatment (1:1 and 1:1:1, respectively) with the Calystegia dahuricus (Herb.) Choisy. The right column shows Representative flow cytometry profiles. The left column shows the percentage of CD107a+ NK cells in four independent experiments. Data are expressed as means ± SD. *P < 0.05; **P < 0.01; ***P < 0.001 by one-way ANOVA with the Dunnett's test.
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