Fig. 1. (A) Cell morphology was observed after 6 h of H2O2 exposure. H9c2 cells were pre-cultured in serum-free medium in the presence or absence of INH2BP (10, 50, and 250 μmol/L) for 1 h, and then stimulated further with 700 μmol/L H2O2 for an additional 6 h. H2O2 resulted in abnormal cell morphology, whereas INH2BP pretreatment resulted in dose-dependent protection from the H2O2-induced morphological changes. Representative images were taken from three independent experiments (magnification, × 40). (B) Effect of INH2BP on H2O2-induced cell death. H9c2 cells were pre-cultured in serum-free medium in the presence or absence of INH2BP (10, 50, and 250 μmol/L) for 1 h, and then stimulated further with 700 μmol/L H2O2 for an additional 6 h, during which the XTT reagent was added at the end of hour 4, and the entire culture mixture was further incubated for 2 h. The absorbance was determined with an enzyme-linked immunosorbent assay reader at a wavelength of 460 nm. Data are means ± standard errors (N = 3). ##P < 0.01 vs. untreated cells; **P < 0.01 vs. H2O2 alone.
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