Fig. 2. (A) Effect of INH2BP on H2O2-induced apoptosis in H9c2 cells. Apoptotic cells were examined under a fluorescence microscope at 200×magnification after Hoechst 33342 staining (scale bar = 50 μm). After 1 h of pretreatment with or without different concentrations of INH2BP (10, 50, and 250 μmol/L), the cells were exposed to H2O2 and stained using Hoechst 33342, and then the cells were visualized under a fluorescence microscope. Apoptotic cells were identified as those with nuclei exhibiting brightly stained condensed chromatin (Hoechst-positive cells). Arrows indicate apoptotic cell nuclei (Hoechst-positive cells). Microscopic images are representative of three independent experiments. (B) Apoptotic rate on H2O2-induced apoptosis in H9c2 cells. After 1 h of pretreatment with or without different concentrations of INH2BP (10, 50, and 250 μmol/L), the cells were exposed to 700 μmol/L H2O2 and stained using Hoechst 33342, and then the cells were visualized under a fluorescence microscope. The apoptotic rate was determined by calculating the percentage of Hoechst-positive cells over total cells. Data are means ± standard errors (N = 3). ##P < 0.01 vs. untreated cells; **P < 0.01 vs. H2O2 alone.
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