Fig. 3. (A) Effect of INH2BP on intracellular reactive oxygen species (ROS) generation. After 1 h of pretreatment with or without INH2BP (10, 50, and 250 μmol/L), the cells were exposed to 700 μmol/L H2O2 for 6 h and assayed for ROS generation using DCF-DA fluorescence. Fluorescence microscopy images of cells fluorescently stained with DCF-DA (magnification, ×40, scale bar = 50 μm). Microscopic images are representative of three independent experiments. (B) Effect of INH2BP on intracellular reactive oxygen species (ROS) generation. After 1 h of pretreatment with or without INH2BP (10, 50, and 250 μmol/L), the cells were exposed to 700 μmol/L H2O2 for 6 h and assayed for ROS generation using DCF-DA fluorescence. Fluorescence was measured with a fluorometer (excitation = 485 nm, emission = 535 nm). Data are representative of three independent experiments and means ± standard errors (N = 3). ##P < 0.01 vs. untreated cells; **P < 0.01 vs. H2O2 alone.
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