Fig. 4. Radical scavenging activity of INH₂BP assessed by ESR. DPPH radical scavenging activity was measured as described by Nanjo et al. (Nanjo et al., 1996). Briefly, 60 μL of 1 mM INH₂BP was added to 60 μL of DPPH (60 μM) in a methanol solution. After the solution was mixed vigorously for 10 sec, it was transferred into a 100 μL Teflon capillary tube, and the scavenging activity of INH₂BP, with regard to DPPH radicals, was measured using an ESR spectrometer. The spin adduct was measured by the ESR spectrometer exactly 2 min later. The experimental conditions were as follows; central field, 3,475 G; modulation frequency, 100 kHz; modulation amplitude, 2 G; microwave power, 5 mW; gain, 6.3 × 10⁵; and temperature, 298 K. DPPH scavenging activity of INH₂BP of ESR spectra. Data are means ± standard errors (n = 3).

Biomed Sci Letters 2022;28:237-46 https://doi.org/10.15616/BSL.2022.28.4.237

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