Fig. 5. Radical scavenging activity of INH2BP assessed by ESR. Superoxide radicals were generated as a result of the ultraviolet irradiation of a riboflavin/ethlylenediaminetetraacetic acid solution. The reaction mixtures that contained 0.1 mL of 0.8 mM riboflavin, 0.1 mL of 1.6 mL EDTA, 0.1 mL of 800 mL DMPO, and 1 mL of 1 mM INH2BP were irradiated for 1 min under an ultraviolet lamp at 365 nm. The measurement conditions were as follows; central field, 3,475 G; modulation frequency, 100 kHz; modulation amplitude, 2 G; microwave power, 1 mW; gain, 6.3 × 105; and temperature, 298 K.Superoxide radical scavenging activity of INH2BP of ESR spectra. Data are means ± standard errors (n = 3).
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