Fig. 6. Radical scavenging activity of INH2BP assessed by ESR. Hydroxyl radicals were generated by an iron-catalyzed Haber-Weiss reaction (i.e., a Fenton-driven Haber-Weiss reaction), and the generated hydroxyl radicals rapidly reacted with nitrone spin-trap DMPO. The resultant DMPO-OH adduct was detected using an ESR spectrometer. Briefly, 0.2 mL of 1 mM INH2BP was mixed with 0.2 mL of DMPO (0.3 M), 0.2 mL of FeSO4 (10 mM), and 0.2 mL of H2O2 (10 mM) in a phosphate buffer solution (pH 7.2), and the resulting solution was transferred into a 100 μL Teflon capillary tube. After 2.5 min, the ESR spectrum was recorded on a JES-FA ESR spectrometer (JEOL Ltd., Tokyo, Japan). The experimental conditions were as follows; central field, 3,475 G; modulation frequency, 100 kHz; modulation amplitude, 2 G; microwave power, 1 mW; gain, 6.3 × 105; and temperature, 298 K.Hydroxyl radical scavenging activity of INH2BP of ESR spectra. Data are means ± standard errors (n = 3).
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