Fig. 4. Effect of SJ on NF-κB activation in atopic dermatitis-like skin lesion and HaCaT cells. (A) The skin tissues were cut out and homogenized. Nuclear lysates were prepared by nuclear extraction kit and he level of nuclear NF-κB p65 in atopic dermatitis-like skin lesion was measured using Western blot analysis. (B) The relative ratio of nuclear NF-κB p65 in skin tissue was determined by densitometry. (C) Cells (5 × 106 cell/well) were treated with SJ (0.1~1 mg/mL) and then stimulated with TNF-α (10 ng/mL) plus IFN-γ (10 ng/mL) for 2 h. The nuclear NF-κB p65 level in cell was measured. (D) The relative ratio of nuclear NF-κB p65 were determined by densitometry. All results were presented in the mean ± S.D of determinations from separate experiments (#P < 0.05 vs. control, ##P < 0.05 vs. DNCB-treated group, *P < 0.05 vs. un-treated cell, **P < 0.05 vs. TNF-α + IFN-γ alone).
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