List of LAMP based ASFV detecting methods
| # | Target gene | Primer | Location | Product size (nt)* | Condition | References | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Name | Sequence (5'→3') | Length (nt) | Start | End | NCBI accession # | ||||||
| 1 | P72 | FIP | TGATAAAGCGCTCGCCGAAGGCGCTTTTGGTTTAATGAGAA | 41 | - | - | MH713612 | 212 | 60℃, 90 min | Dokphut et al., 2021 | |
| BIP | GCTTGCATCGCAAAAGGATTTGCTATAAAACGTGACTGGC | 40 | - | - | |||||||
| Floop | CTGAGGGAATAGCAAGGTTCAC | 22 | - | - | |||||||
| F3 | ACTATCAGCCCCCTCTTG | 18 | 960 | 977 | |||||||
| B3 | GCGTATATTGCGTCTACTGG | 20 | 766 | 785 | |||||||
| 2 | P72 | 4FIP | TGATCGGATACGTAACGGGATAGAGATACAGCTCTTC | 37 | - | - | MN207061.1 | 244 | 65℃, 40 min | Wu et al., 2020 (Shaanxi innolever biological technology Co. Ltd.) | |
| 4BIP | CCGTAACTGCTCATGGTACGTAGTGGAAGGGTAT | 34 | - | - | |||||||
| 4LoopF | ATAGATGAACATGCGTC | 17 | - | - | |||||||
| 4LoopB | AGTTCTGCAGCTCTTA | 16 | - | - | |||||||
| 4F3 | ACGCAGAGATAAGCTT | 16 | 1,508 | 1,523 | |||||||
| 4B3 | AAGGTAATCATCATCGC | 17 | 1,735 | 1,751 | |||||||
| 3 | FIP | GCAGAACTTTGATGGAAACTTATCGTTAAAAACATTTCCGTAACTGCT | 48 | - | - | AY261361 | 195 | 65℃, 50 min | James et al., 2010 | ||
| BIP | GCTCTTACATACCCTTCCACTACGGTAATCATCATCGCACCCG | 43 | - | - | |||||||
| F3 | CGTTACGTATCCGATCACATT | 21 | 102,297 | 102,317 | |||||||
| B3 | TATTCCTCCCGTGGCTTC | 18 | 102,123 | 102,140 | |||||||
| 4 | P72 | B-FIP | CATGAGCAGTTACGGAAATGTTTTTTGTTCATCTATATCTGATATTAGCC | 50 | - | - | MH910495.1 | 212 | 63℃, 50 min | Center for animal disease control and prevention of Beijing | |
| B-BIP | AATTTCCATCAAAGTTCTGCAGCTTTTAATCGCATTGCCTCC | 42 | - | - | |||||||
| B-LB | GTAATGTGATCGGATACGTAACGG | 24 | - | - | |||||||
| B-F3 | TACAGCTCTTCCAGACGC | 18 | 103,984 | 104,001 | |||||||
| B-B3 | TAATCATCATCGCACCCG | 18 | 103,790 | 103,807 | |||||||
| 5 | P72 | A-FIP | AGCTGCAGAACTTTGATGGAAATTTAAACATTTCCGTAACTGCTCA | 46 | - | - | MH910495.1 | 208 | 63℃, 50 min | ||
| A-BIP | ACGGAGGCAATGCGATTAAAATATTCCTCCCGTGGCTTC | 39 | - | - | |||||||
| A-LB | CCGATGATCCGGGTGCGAT | 19 | - | - | |||||||
| A-F3 | CGTATCCGATCACATTACCT | 20 | 103,932 | 103,951 | |||||||
| A-B3 | ATATGACCACTGGGTTGG | 18 | 103,744 | 103,761 | |||||||
| 6 | No data | 63℃, 30 min & 80℃, 2 min | MmisoASFV detection kit (Mmonitor, Korea) | ||||||||
*Amplicon sizes were using LAMP outer PCR primer set
