Fig. 1. ZJM promotes myogenic differentiation without cytotoxicity.
(A) C2C12 cells were cultured for 3 days at the indicated concentration of ZJM. Cell viability was analyzed by XTT assay. (B) Giemsa staining was performed on the 5 day of C2C12 cells differentiation to measure changes in myotube morphology, such as myotube length and diameter. (C) Immunofluorescence staining of MyHC (green color), and the nuclei marker, DAPI (blue color), showed myotube formation in the C2C12 cells. (D) Relative myotube diameters were measured. **P < 0.01 vs. DMSO control.
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