Biomedical Science Letters

eISSN 2288-7415

Download original image
Fig. 2. ZJM upregulates myogenic differentiation markers in C2C12 muscle cells.
C2C12 myoblasts, cultured with ZJM (0.5, 1 and 5 ng/mL) for 7 days. (A) mRNA expression levels of MyHC1, MyoD and myogenin were analyzed by real-time quantitative RT-PCR. (B) The cell lysates were analyzed by western blotting with antibodies. β-actin was used as internal control. (C) Quantification of relative ratio of band intensity was performed using Image J software. Data represent means ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 vs. DMSO control.
Biomed Sci Letters 2023;29:26-33
© 2023 Biomed Sci Letters