Fig. 2. ZJM upregulates myogenic differentiation markers in C2C12 muscle cells.
C2C12 myoblasts, cultured with ZJM (0.5, 1 and 5 ng/mL) for 7 days. (A) mRNA expression levels of MyHC1, MyoD and myogenin were analyzed by real-time quantitative RT-PCR. (B) The cell lysates were analyzed by western blotting with antibodies. β-actin was used as internal control. (C) Quantification of relative ratio of band intensity was performed using Image J software. Data represent means ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 vs. DMSO control.
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