Biomedical Science Letters

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Fig. 3. ZJM stimulates C2C12 muscle cells on day 7 of myogenic differentiation.
C2C12 myoblasts were cultured in a DM with ZJM (5 ng/mL) for indicated different time points. (A) mRNA expression levels of MyHC1, MyoD and myogenin were examined by real-time quantitative RT-PCR. (B) The cell lysates were examined by Western blotting with antibodies. β-actin was used as internal control. (C) Quantification of relative ratio of band intensity was performed using Image J software. Data represent means ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 vs. DMSO control at 0 min; #P < 0.05, ##P < 0.01, ###P < 0.001 as compared with DMSO control at the indicated time.
Biomed Sci Letters 2023;29:26-33
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