Fig. 4. ZJM down-regulates muscle degradation markers in C2C12 muscle cells.
C2C12 muscle cells were cultured in DM with 0.5, 1 and 5 ng/mL ZJM for 7 days. (A) The cell harvest were reviewed by western blotting with antibodies. β-actin was used as internal control. (B) Quantification of relative ratio of band intensity was performed using Image J software. Data represent means ± SD (n = 3). *P < 0.05, ***P < 0.001 vs. DMSO control.
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