Fig. 5. ZJM reduces muscle degradation in C2C12 muscle cells on day 7 of myogenic differentiation.
C2C12 myoblasts were cultured in a DM with ZJM (5 ng/mL) for indicated different time points. (A) The cell dissolved matter were tested by western blotting with antibodies. β-actin was used as internal control. (B) Quantification of relative ratio of band intensity was performed using Image J software. Data represent means ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 vs. control at 0 min; #P < 0.05, ##P < 0.01 as compared with DMSO control at the indicated time.
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