RNA concentrations and purities in the formalin fixatives and the alternative fixative
Fixative | Organ | RNA concentration (μL) | A260/280 ratio |
---|---|---|---|
Formaldehyde solution 10% = (EP) |
Kidney | 27.9 | 1.8 |
Spleen | 150.5 | 1.9 | |
Liver | 159.8 | 1.8 | |
Mean ± SD | 112.7±73.6 | 1.8±0.05 | |
Formaldehyde solution (Sigma-Aldrich) | Kidney | 27.9 | 1.7 |
Spleen | 120.8 | 1.9 | |
Liver | 144.3 | 2.0 | |
Mean ± SD | 97. 7±61.5 | 1.8±0.15 | |
Core-Fix (Corebiotech) | Kidney | 39.5 | 1.7 |
Spleen | 125.5 | 2.0 | |
Liver | 138.6 | 2.0 | |
Mean ± SD | 101.2±53.8 | 1.9±0.17 |