Fig. 4. TA decreases the expression of myogenic markers during myoblast differentiation.
C2C12 cells were cultured in DM and were treated with or without 50 or 200 μg of TA. The cells were harvested on differentiation days 1, 3, and 5. qRT-PCR was performed to assess the expression of MyoD, myogenin, MEF2C, MCK, and MyHC during myoblast differentiation. Results are expressed as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 compared with the vehicle controls.
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